Mitofilin antibody
Principal name
Mitofilin antibody
Alternative names for Mitofilin antibody
IMMT, HMP, PIG4, Mitochondrial marker inner membrane protein, p87/89, Cell proliferation-inducing gene 4 protein
Ncbi ID
NP_001093639, 9606, NP_001093639.1, ACE87159.1, NP_006830, NP_083949.2, NM_029673, NM_006839, NM_001100169, NM_001100170
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of Mitofilin antibody
- Kit Component:
- - KN308310G1, Immt gRNA vector 1 in pCas-Guide vector
- KN308310G2, Immt gRNA vector 2 in pCas-Guide vector
- KN308310D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of IMMT Antibody (Center) (AP17502PU-N) in Hela, NCI-H460, CEM cell line lysates (35ug/lane). IMMT (arrow) was detected using the purified Pab.
IMMT polyclonal antibody (A01), Lot # 060503JCS1 Western Blot analysis of IMMT expression in A-431 ( Cat # L015V1 ).
Immunofluorescence of IMMT on HeLa cells with IMMT polyclonal antibody ( Cat # PAB12412 ) at 1 : 500 dilution labeling mitochondria.
Western blot of IMMT in dog testis total protein extract using IMMT polyclonal antibody ( Cat # PAB12412 ) at 1 : 2000 dilution.
Western blot analysis of Mitofilin expression in HepG2 (A); HeLa (B); SP2/0 (C); PC12 (D) whole cell lysates.
Western blot of dog testis total protein extract 1:2000 dilution of antibody
Immunofluorescence of HeLa cells with antibody 1:500 dilution labeling mitochondria
Predicted band size: 84 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using IMMT antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted band size: 84 kDa . Positive control: Hela and 231 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Hela cell lysate Lane2: 231 cell lysate Lysates: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using IMMT antibody at dilution 1/12, on the right is treated with the fusion protein. (Original magnification: x200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA301674 | Mitofilin antibody |
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| Rabbit Polyclonal Antibody against Mitofilin - Mitochondrial Marker | |||||||
 |
Rabbit | IgG | Purified | Can, Fe, Hu, Ms, Rt | WB |
0.1 ml /
€475.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA323243 | Mitofilin antibody |
||||||
| Anti-IMMT Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA323244 | Mitofilin antibody |
||||||
| Anti-IMMT Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP17502PU-N | Mitofilin (Center) antibody |
||||||
 |
Rabbit | Hu | WB |
0.4 ml /
€415.00
|
|||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760022 | Mitofilin (full length, N-term HIS tag, transcript variant 1) |
||||
| Recombinant protein of human inner membrane protein, mitochondrial (IMMT), nuclear gene encoding mitochondrial protein, transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR216091L2V | Lenti ORF particles, Immt (GFP-tagged) - Mouse inner membrane protein, mitochondrial (Immt), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,550.00
|
|
| OriGene Technologies, Inc. | ||
| MR216091L1V | Lenti ORF particles, Immt (Myc-DDK-tagged) - Mouse inner membrane protein, mitochondrial (Immt), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,390.00
|
|
| OriGene Technologies, Inc. | ||
