MKNK1 antibody
Principal name
MKNK1 antibody
Alternative names for MKNK1 antibody
MNK1, MAP kinase-interacting serine/threonine-protein kinase 1
Ncbi ID
9606, NP_001129025.1, NP_001129025, NM_003684, BAA19885.1, NM_001135553, NP_003675, NP_067436.1, NM_021461, NM_001044267, NM_198973
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair)
Background of MKNK1 antibody
- Kit Component:
- - KN310125G1, Mknk1 gRNA vector 1 in pCas-Guide vector
- KN310125G2, Mknk1 gRNA vector 2 in pCas-Guide vector
- KN310125D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of MKNK1 expression in transfected 293T cell line by MKNK1 monoclonal antibody (M04), clone 5E12.Lane 1: MKNK1 transfected lysate(51.3 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged MKNK1 is approximately 1ng/ml as a capture antibody.
Western Blot analysis of MKNK1 expression in transfected 293T cell line by MKNK1 monoclonal antibody (M05), clone 5E2.Lane 1: MKNK1 transfected lysate(51.3 KDa).Lane 2: Non-transfected lysate.
Immunoperoxidase of monoclonal antibody to MKNK1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to MKNK1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of MKNK1 expression in transfected 293T cell line by MKNK1 monoclonal antibody (M06), clone 3E1.Lane 1: MKNK1 transfected lysate(51.3 KDa).Lane 2: Non-transfected lysate.
MKNK1 monoclonal antibody (M06), clone 3E1 Western Blot analysis of MKNK1 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged MKNK1 is approximately 3ng/ml as a capture antibody.
Western Blot analysis of MKNK1 expression in transfected 293T cell line by MKNK1 monoclonal antibody (M07), clone 2C5.Lane 1: MKNK1 transfected lysate(51.3 KDa).Lane 2: Non-transfected lysate.
Immunoperoxidase of monoclonal antibody to MKNK1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged MKNK1 is approximately 3ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to MKNK1 on HeLa cell. [antibody concentration 10 ug/ml]
MKNK1 monoclonal antibody (M08), clone 2H8 Western Blot analysis of MKNK1 expression in HeLa ( Cat # L013V1 ).
Western Blot analysis of MKNK1 expression in transfected 293T cell line by MKNK1 monoclonal antibody (M08), clone 2H8.Lane 1: MKNK1 transfected lysate(51.3 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MKNK1 expression in transfected 293T cell line by MKNK1 monoclonal antibody (M09), clone 3C9.Lane 1: MKNK1 transfected lysate(51.342 KDa).Lane 2: Non-transfected lysate.
MKNK1 monoclonal antibody (M10), clone 4G6. Western Blot analysis of MKNK1 expression in NIH/3T3 ( Cat # L018V1 ).
MKNK1 monoclonal antibody (M10), clone 4G6. Western Blot analysis of MKNK1 expression in Raw 264.7.
Western Blot analysis of MKNK1 expression in transfected 293T cell line by MKNK1 monoclonal antibody (M11), clone 3G6.Lane 1: MKNK1 transfected lysate(51.342 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MKNK1 expression in transfected 293T cell line by MKNK1 monoclonal antibody (M12), clone 3E10.Lane 1: MKNK1 transfected lysate(51.342 KDa).Lane 2: Non-transfected lysate.
Immunoperoxidase of monoclonal antibody to MKNK1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to MKNK1 on HeLa cell. [antibody concentration 10 ug/ml]
MKNK1 monoclonal antibody (M14), clone 2F12. Western Blot analysis of MKNK1 expression in Raw 264.7. (Isoform : 51.342, 47.402, 39.103 kD)
Western blot (WB) analysis of Mnk1 antibody (Cat.-No.: AP06753PU-N) in extracts from HUVEC cells.
Immunohistochemistry (IHC) analyzes of Mnk1 antibody (Cat.-No.: AP06753PU-N) on paraffin-embedded sections.
The MKNK1 polyclonal antibody ( Cat # PAB2708 ) is used in Western blot to detect MKNK1 in A2058 cell lysate.
Immunoblot analysis of cell lysates. Lane 1 : HeLa cell lysate. Lane 2 : MCF-7 cell lysate. Lane 3 : NIH/3T3 cell lysate. Lane 4 : H9C2 cell lysate.
The anti-MKNK1 Pab is used in Western blot to detect MKNK1 in A2058 tissue lysate
Proximity Ligation Analysis (PLA) of protein-protein interactions between MKNK1 and PAK2 HeLa cells were stained with anti-MKNK1 rabbit purified polyclonal 1:1200 and anti-PAK2 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
MKNK1 MaxPab rabbit polyclonal antibody. Western Blot analysis of MKNK1 expression in human kidney.
Western Blot analysis of MKNK1 expression in transfected 293T cell line (H00008569-T02) by MKNK1 MaxPab polyclonal antibody.Lane 1: MKNK1 transfected lysate(51.30 KDa).Lane 2: Non-transfected lysate.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to MKNK1 on HeLa cell. [antibody concentration 10 µg/ml]
Western Blot analysis of MKNK1 expression in transfected 293T cell line by MKNK1 monoclonal antibody, clone 2H8.
MKNK1 monoclonal antibody, clone 2H8 Western Blot analysis of MKNK1 expression in HeLa.
Detection limit for recombinant GST tagged MKNK1 is approximately 3ng/ml as a capture antibody.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to MNK1 on HeLa cell. [antibody concentration 10 µg/ml]
MNK1 monoclonal antibody, clone 2F12. Western Blot analysis of MNK1 expression in Raw 264.7. (Isoform : 51.342, 47.402, 39.103 kD)
Human 293T; WB Suggested Anti-MKNK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate. MKNK1 is supported by BioGPS gene expression data to be expressed in HEK293T.; MKNK1 antibody - C-terminal region (ARP60345_P050) in Human 293T cells using Western Blot
Human A549; WB Suggested Anti-MKNK1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: A549 Whole Cell. MKNK1 is supported by BioGPS gene expression data to be expressed in A549.; MKNK1 antibody - N-terminal region (ARP60346_P050) in Human A549 cells using Western Blot
Western blot analysis of MNK1 expression in MCF7 (A); mouse brain (B) whole cell lysates.
Immunohistochemical analysis of MNK1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MNK1 antibodyThe picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using Mnk1 (Ab-385) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HepG2 cells (Lane 2), K562 cells (Lane 3) and Jurket cells (Lane 4), using Mnk1 (Ab-385) Antibody. The lane on the left is treated with systhesized peptide.
Western blot analysis of extracts from HuvEc cells, using MKNK1 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-MKNK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate MKNK1 is supported by BioGPS gene expression data to be expressed in HEK293T
WB Suggested Anti-MKNK1 Antibody; Titration: 1.0 ug/ml; Positive Control: A549 Whole Cell MKNK1 is supported by BioGPS gene expression data to be expressed in A549
Western blot analysis on HeLa cell lysate using MKNK1 Antibody
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM31082PU-N | MKNK1 (1-466) antibody |
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Mouse | IgG1 | 2H8 | Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA313002 | MKNK1 antibody |
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| Rabbit polyclonal anti-MNK1 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA313003 | MKNK1 antibody |
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| Rabbit polyclonal Mnk1 (Ab-385) antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA313004 | MKNK1 antibody |
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| Rabbit polyclonal anti-MKNK1 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347568 | MKNK1 antibody |
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| Rabbit Polyclonal Anti-MKNK1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM31087PU-N | MKNK1 antibody |
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 |
Mouse | IgG2a | 2F12 | Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP06753PU-N | MKNK1 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | P, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP13792PU-N | MKNK1 (N-term) antibody |
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Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301149 | MKNK1 (transcript variant 1) |
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| Recombinant protein of human MAP kinase interacting serine/threonine kinase 1 (MKNK1), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY418502 | MKNK1 overexpression lysate |
|
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY427613 | MKNK1 overexpression lysate |
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0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206550L1V | Lenti ORF particles, Mknk1 (Myc-DDK-tagged) - Mouse MAP kinase-interacting serine/threonine kinase 1 (Mknk1), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR212832L2V | Lenti ORF particles, Mknk1 (GFP-tagged ORF) - Rat MAP kinase-interacting serine/threonine kinase 1 (Mknk1), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR212832L1V | Lenti ORF particles, Mknk1 (Myc-DDK-tagged ORF) - Rat MAP kinase-interacting serine/threonine kinase 1 (Mknk1), 200 uL, >10^7 TU/mL |
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|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
