Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » MKNK2 antibody

MKNK2 antibody

(reset)

Principal name

MKNK2 antibody

Alternative names for MKNK2 antibody

GPRK7, MNK2, MAP kinase-interacting serine/threonine-protein kinase 2

SwissProt ID

Q5U2N4 (Rat), Q8CDB0 (Mouse), Q9HBH9 (Human)

Gene ID

2872 (MKNK2), 17347 (Mknk2), 299618

Ncbi ID

9606, NP_951009, NM_017572, NP_951009.1, NP_060042, NP_060042.2, NP_067437.1, NM_021462, NM_001011985

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of MKNK2 antibody

MAP kinase-interacting kinase 1 (Mnk1) and Mnk2, members of the Ser/Thr protein kinase family, bind tightly to the growth factor-regulated MAP kinases, Erk1 and Erk2. Erk and p38 phosphorylate MNK1 and Mnk2, which stimulates their in vitro kinase activity toward a substrate, eukaryotic initiation factor-4E (eIF-4E). Overexpression of Mnk2 results in increased phosphorylation of endogenous eIF-4E, showing that it can act as an eIF-4E kinase in vivo. Mnk2 may play a role in the response to environmental stress and cytokines. This ubiquitiously expressed protein appears to regulate transcription by phosphorylating eIF-4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Expression of active mutants of MNK1 and MNK2 in 293 cells diminishes cap-dependent translation relative to cap-independent translation in a transient reporter assay. Human Mnk2 is homologous to murine Mnk2 (approximately 94% identical) and human Mnk1 (71% identical). In vitro phosphorylation studies show that Mnk2 is a significantly better substrate than Mnk1 for extracellular signal-regulated kinase 2 (Erk2), p38MAPKalpha, and p38MAPKbeta. Mnk2 has also been shown to interact with the C-terminal regions of eIF-4G1 and eIF-4G2.

General readings

Wang,P., (2006) Sci. China, C, Life Sci. 49 (3), 265-273

H00002872-M04 - MKNK2
Immunofluorescence of monoclonal antibody to MKNK2 on HeLa cell. [antibody concentration 25 ug/ml]
H00002872-M04 - MKNK2
Western Blot analysis of MKNK2 expression in transfected 293T cell line by MKNK2 monoclonal antibody (M04), clone 4F11.Lane 1: MKNK2 transfected lysate(46.7 KDa).Lane 2: Non-transfected lysate.
H00002872-M04 - MKNK2
MKNK2 monoclonal antibody (M04), clone 4F11 Western Blot analysis of MKNK2 expression in HeLa ( Cat # L013V1 ).
H00002872-M04 - MKNK2
Detection limit for recombinant GST tagged MKNK2 is approximately 1ng/ml as a capture antibody.
H00002872-M07 - MKNK2
Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK14 and MKNK2 HeLa cells were stained with anti-MAPK14 rabbit purified polyclonal 1:1200 and anti-MKNK2 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
H00002872-M07 - MKNK2
Western Blot analysis of MKNK2 expression in transfected 293T cell line by MKNK2 monoclonal antibody (M07), clone 2A10.Lane 1: MKNK2 transfected lysate(46.7 KDa).Lane 2: Non-transfected lysate.
H00002872-M07 - MKNK2
MKNK2 monoclonal antibody (M07), clone 2A10 Western Blot analysis of MKNK2 expression in K-562 ( Cat # L009V1 ).
H00002872-M07 - MKNK2
MKNK2 monoclonal antibody (M07), clone 2A10. Western Blot analysis of MKNK2 expression in PC-12 ( Cat # L012V1 ).
H00002872-M07 - MKNK2
MKNK2 monoclonal antibody (M07), clone 2A10. Western Blot analysis of MKNK2 expression in NIH/3T3 ( Cat # L018V1 ).
H00002872-M07 - MKNK2
Detection limit for recombinant GST tagged MKNK2 is approximately 0.3ng/ml as a capture antibody.
H00002872-A02 - MKNK2
MKNK2 polyclonal antibody (A02), Lot # 060717JCS1 Western Blot analysis of MKNK2 expression in HeLa ( Cat # L013V1 ).
PAB4593 - MKNK2
The MKNK2 polyclonal antibody ( Cat # PAB4593 ) is used in Western blot to detect MKNK2 in mouse lung tissue lysate .
H00002872-D01P - MKNK2
Western Blot analysis of MKNK2 expression in transfected 293T cell line (H00002872-T01) by MKNK2 MaxPab polyclonal antibody.Lane 1: MKNK2 transfected lysate(46.70 KDa).Lane 2: Non-transfected lysate.
AP13616PU-N - MKNK2
The anti-MKNK2 Pab is used in Western blot to detect MKNK2 in mouse lung tissue lysate.
AP13794PU-N - MKNK2
Western blot analysis of MNK2 (MKNK2) antibody (C-term) in 293 cell line lysates (35ug/lane). MNK2 (arrow) was detected using the purified Pab.
ARP55352_P050 - MKNK2
Human Spleen; MKNK2 antibody - N-terminal region (ARP55352_P050) in Human Spleen cells using Immunohistochemistry
ARP55352_P050 - MKNK2
Human Placenta; Immunohistochemistry with Human Placenta lysate tissue at an antibody concentration of 5.0ug/ml using anti-MKNK2 antibody (ARP55352_P050); MKNK2 antibody - N-terminal region (ARP55352_P050) in Human Placenta cells using Immunohistochemistry
ARP55352_P050 - MKNK2
Human 721_B; WB Suggested Anti-MKNK2 Antibody Titration: 1 ug/ml. Positive Control: 721_B cell lysate. MKNK2 is supported by BioGPS gene expression data to be expressed in 721_B.; MKNK2 antibody - N-terminal region (ARP55352_P050) in Human 721_B cells using Western Blot
TP316704 - MKNK2
TP760279 - MKNK2
TA313959 - MKNK2
Western blot analysis of extracts from HeLa cells, using MNK2 antibody.The lane on the right is treated with the synthesized peptide.
TA333339 - MKNK2
WB Suggested Anti-MKNK2 Antibody Titration: 1 ug/ml; Positive Control: 721_B cell lysateMKNK2 is supported by BioGPS gene expression data to be expressed in 721_B
TA333339 - MKNK2
Immunohistochemistry with Human Placenta lysate tissue at an antibody concentration of 5.0 ug/ml using anti-MKNK2 antibody
TA333339 - MKNK2
Human Spleen
  1. Quick-Filters:
  2. Cited (1)

8 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MKNK2 antibody
  Rabbit polyclonal anti-MNK2 antibody  
Western blot analysis of extracts from HeLa cells, using MNK2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

MKNK2 (N-term) antibody
The anti-MKNK2 Pab is used in Western blot to detect MKNK2 in mouse lung tissue lysate. Rabbit Ig Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

MKNK2 (C-term) antibody
Western blot analysis of MNK2 (MKNK2) antibody (C-term) in 293 cell line lysates (35ug/lane). MNK2 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MKNK2 (transcript variant 2)
  Recombinant protein of human MAP kinase interacting serine/threonine kinase 2 (MKNK2), transcript variant 2  
MKNK2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

MKNK2 (full length, N-term HIS tag, transcript variant 1)
  Recombinant protein of human MAP kinase interacting serine/threonine kinase 2 (MKNK2), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
MKNK2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

MKNK2 overexpression lysate
MKNK2 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.
 

Published recently!

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Mknk2 (GFP-tagged ORF) - Rat MAP kinase-interacting serine/threonine kinase 2 (Mknk2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Mknk2 (GFP-tagged ORF) - Rat MAP kinase-interacting serine/threonine kinase 2 (Mknk2), 200 uL, >10^7 TU/mL  
200 µl / €1,120.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Mknk2 (Myc-DDK-tagged ORF) - Rat MAP kinase-interacting serine/threonine kinase 2 (Mknk2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Mknk2 (Myc-DDK-tagged ORF) - Rat MAP kinase-interacting serine/threonine kinase 2 (Mknk2), 200 uL, >10^7 TU/mL  
200 µl / €1,010.00
  OriGene Technologies, Inc.
 

8 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (3)
  3. Protein | Growth Factor (2)
  4. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (3)
Target
  1. MKNK2 (5)
Property
  1. C-term (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (1)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (1)
Presentation
  1. Purified (3)
Host
  1. Rabbit (3)
Images
  1. 1 image (5)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
Species (Protein)
  1. Human (2)
Source
  1. E. coli (1)
  2. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (8)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn