Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » MLCK antibody

MLCK antibody

(reset)

Principal name

MLCK antibody

Alternative names for MLCK antibody

MYLK3, Putative myosin light chain kinase 3

SwissProt ID

Q32MK0 (Human), Q96DV1

Gene ID

91807 (MYLK3)

Ncbi ID

9606, NP_872299, NM_182493, NP_872299.2

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rt (Rat)

Available hosts

Rabbit

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of MLCK antibody

MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

General readings

Al-Sadi, R. et al., J. Immunol. 180(8): 5653-5661 (2008)
Chan, J.Y. et al., Circ. Res. 102(5): 571-580 (2008)
Seguchi, O. et al., J. Clin. Invest. 117(10): 2812-2824 (2007)

AP14866PU-N - MLCK
(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MLCK antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP14866PU-N - MLCK
(LEFT)Western blot analysis of anti-MLCK Antibody (N-term) in A375 cell line lysates (35ug/lane). MLCK(arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of anti-MLCK Antibody (N-term) in mouse heart tissue lysates (35ug/lane). MLCK(arrow) was detected using the purified Pab.
AP52792PU-N - MLCK
Western blot analysis in K562 cell line lysates (35ug/lane) using MLCK antibody Cat.-No. AP52792PU-N. This demonstrates this antibody detected the MLCK protein (arrow).
TA333399 - MLCK
Host: Rabbit; Target Name: MYLK3; Sample Tissue: HepG2 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml.
TA349605 - MYLK3
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (MYLK3 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
TA349605 - MYLK3
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (MYLK3 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)

4 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MLCK antibody
  Rabbit Polyclonal Anti-MYLK3 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (MYLK3 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

MLCK (N-term) antibody
(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MLCK antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

MLCK (Center) antibody
Western blot analysis in K562 cell line lysates (35ug/lane) using MLCK antibody Cat.-No. AP52792PU-N. This demonstrates this antibody detected the MLCK protein (arrow). Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Lysates

Catalog No.    

MYLK3 overexpression lysate
MYLK3 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

4 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (3)
Clonality
  1. Polyclonal (3)
Target
  1. MLCK (3)
Property
  1. Center (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (1)
Applications
  1. Paraffin Sections (P) (3)
  2. Western blot / Immunoblot (WB) (2)
  3. Enzyme Immunoassay (E) (1)
Presentation
  1. Purified (2)
  2. Aff - Purified (1)
Host
  1. Rabbit (3)
Images
  1. 1 image (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. IHC (1)
  3. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (4)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn