MLH1 antibody
Principal name
MLH1 antibody
Alternative names for MLH1 antibody
DNA mismatch repair protein Mlh1, COCA2
Ncbi ID
NP_000240.1, 9606, NP_000240, NP_000239, NM_000249, NM_031053, NM_001167618, NM_001167619, NM_001167617
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of MLH1 antibody
DNA-mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. Lack of a functional DNA-mismatch repair pathway is a commoncharacteristic of several different types of human cancers, either due to anMMR gene mutation or promoter-methylation gene silencing. MLH1 is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is an integralpart of the protein complex responsible for mismatch repair expressed inlymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid andgall bladder, and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectalcancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. MLH1 also plays a role in meiotic recombination.
SP2066P staining MLH1 in human gastric cancer. Formalin fixed, paraffin processed tissue.
Detection of human MLH1 in HeLa cell nuclear extract by Western blotting and immunoprecipitation using Rabbit anti Human MLH1 (SP2066P)
Immunoperoxidase of monoclonal antibody to MLH1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1ug/ml]
Proximity Ligation Analysis (PLA) of protein-protein interactions between MSH2 and MLH1 HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-MLH1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Immunofluorescence of monoclonal antibody to MLH1 on HeLa cell. [antibody concentration 10 ug/ml]
MLH1 monoclonal antibody (M02), clone M1 Western Blot analysis of MLH1 expression in HeLa NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged MLH1 is approximately 0.03ng/ml as a capture antibody.
Human colon carcinoma stained with anti-MLH1 antibody
Immunohistochemical staining of MLH1 on formalin fixed, paraffin embedded human colon cancer with MLH1 polyclonal antibody.
Human 293T; . Lanes: Lane1: hMLH1 tranfected 293 cells. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:5000. Gene Name: MLH1. Submitted by: Galina Petukhova, USUHS. MLH1 is supported by BioGPS gene expression data to be expressed in HEK293T.; MLH1 antibody - N-terminal region (ARP56076_P050) in Human 293T cells using Western Blot
Human Placenta; WB Suggested Anti-MLH1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Placenta; MLH1 antibody - N-terminal region (ARP56076_P050) in Human Placenta cells using Western Blot
Western blot (WB) analysis of MLH1 antibody (Cat.-No.: AP20490PU-N) in extracts from K562 cells.
Human Small Intestine (formalin-fixed, paraffin-embedded) stained with MLH1 antibody AM20522SU-N followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis in K562 cell line lysates (35ug/lane) using MLH1 antibody Cat.-No. AP52704PU-N. This demonstrates the MLH1 antibody detected the MLH1 protein (arrow).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram) using MLH1 antibody Cat.-No. AP52704PU-N, followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
Formalin-Fixed, Paraffin-Embedded Human colon carcinoma stained with MLH1 antibody Cat.-No. AM10114SU-N using peroxidase conjugate and DAB chromogen. Note the nuclear staining of tumor cells.
Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using MLH1 antibody Cat.-No AM06257SU-N
Confocal Immunofluorescence analysis of Hela cells using MLH1 antibody Cat.-No AM06257SU-N (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using MLH1 antibody Cat.-No AM06257SU-N against Hela (Lane 1), MCF-7 (Lane 2) and A549 (Lane 3), Jurkat (Lane 4), 2R75 (Lane 5) and COS (Lane 6) cell lysate.
Immunofluorescent analysis of MLH1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of MLH1 expression in KNRK (A); HeLa (B); A431 (C); SW480 (D) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MLH1 rabbit polyclonal antibody. (TA590013)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-MLH1 rabbit polyclonal antibody. (TA590013)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-MLH1 rabbit polyclonal antibody. (TA590013)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MLH1 rabbit polyclonal antibody. (TA590013)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MLH1 rabbit polyclonal antibody. (TA590013)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-MLH1 rabbit polyclonal antibody. (TA590013)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MLH1 (RC201607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MLH1.
ELISA: MLH1 Antibody
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MLH1 mouse monoclonal antibody. (TA805066; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western blot analysis of extracts from K562 cells, using MLH1 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts of various cell lines, using MLH1 antibody.
WB Suggested Anti-MLH1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Placenta
Lanes: Lane1: hMLH1 tranfected 293 cells; Primary Antibody Dilution: ; 1:500; Secondary Antibody: ; Anti-rabbit HRP; Secondary Antibody Dilution: ; 1:5000; Gene Name: ; MLH1; Submitted by: ; Galina Petukhova, USUHS; MLH1 is supported by BioGPS gene expres
Western blot detection of MLH1 in Hela,293T and Jurkat cell lysates using MLH1 mouse mAb (1:500 diluted).Predicted band size:85KDa.Observed band size:85KDa.
Human Colon Carcinoma stained with anti-MLH1 antibody.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (MLH1 Antibody) at dilution 1/65, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| UM800083 | MLH1 antibody |
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| MLH1 mouse monoclonal antibody,clone UMAB191 | |||||||
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Mouse | IgG1 | UMAB191 | Purified | Hu | P |
0.1 ml /
€468.00
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
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| UM870083 | MLH1 antibody |
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| MLH1 mouse monoclonal antibody,clone UMAB191 | |||||||
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Mouse | IgG1 | UMAB191 | Purified | Hu | P |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
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| TA590013 | MLH1 antibody |
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| Rabbit Polyclonal MLH1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 mg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody! |
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| TA311753 | MLH1 antibody |
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| Rabbit polyclonal anti-MLH1 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA326801 | MLH1 antibody |
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| Rabbit anti-MLH1 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€335.00
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|
| OriGene Technologies, Inc. | |||||||
| TA347003 | MLH1 antibody |
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| Mouse Monoclonal MLH1 Antibody | |||||||
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Mouse | IgG2b | 2B3-H6-C11 | Purified | WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA349405 | MLH1 antibody |
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| Rabbit Polyclonal Anti-MLH1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA805066 | MLH1 antibody |
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| MLH1 mouse monoclonal antibody, clone OTI1C1 (formerly 1C1) | |||||||
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Mouse | IgG1 | OTI1C1 | Purified | Hu | P |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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| AM06257SU-N | MLH1 antibody |
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Mouse | IgG1 | 4C9C7 | Ascites | Hu, Mky | ICC/IF, P, WB |
0.1 ml /
€380.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AM10114SU-N | MLH1 antibody |
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Mouse | IgG1 | G168-728 | Supernatant | Hst, Hu, Ms, Rt | P |
1 ml /
€690.00
|
| OriGene Technologies GmbH | |||||||
| AM20522SU-N | MLH1 antibody |
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Mouse | IgG1 | n.a | Ascites | Hu, Mky | E, ICC/IF, P, WB |
50 µl /
€425.00
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| OriGene Technologies GmbH | |||||||
| AP20490PU-N | MLH1 antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 mg /
€370.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP52704PU-N | MLH1 (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu | F, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| DM430 | MLH1 antibody |
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Mouse | IgG1 | G168-15 | Supernatant | Hst, Hu, Ms, Rt | C, P |
1 ml /
€950.00
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| OriGene Technologies GmbH | |||||||
| DM430-05 | MLH1 antibody |
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Mouse | IgG1 | G168-15 | Supernatant | Hst, Hu, Rt | P |
0.5 ml /
€600.00
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| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN201607 | MLH1 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN201607BN | MLH1 - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN201607LP | MLH1 - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN201607RB | MLH1 - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301607 | MLH1 |
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| Recombinant protein of human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||