MLL4 antibody
Principal name
MLL4 antibody
Alternative names for MLL4 antibody
WBP7, HRX2, KIAA0304, KMT2D, MLL2, TRX2, Histone-lysine N-methyltransferase MLL4, Myeloid/lymphoid or mixed-lineage leukemia protein 4, WW domain-binding protein 7, Trithorax homolog 2, Lysine N-methyltransferase 2D
Ncbi ID
9606, Q9UMN6-2, NP_083550, NP_055542, NP_055542.1, NP_083550.2
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of MLL4 antibody
- Kit Component:
- - KN308950G1, Kmt2b gRNA vector 1 in pCas-Guide vector
- KN308950G2, Kmt2b gRNA vector 2 in pCas-Guide vector
- KN308950D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Huntsman DG, Chin SF, Muleris M, Batley SJ, Collins VP, Wiedemann LM, Aparicio S,
Caldas C.
MLL2, the second human homolog of the Drosophila trithorax gene, maps to 19q13.1 and
is amplified in solid tumor cell lines.
Oncogene. 1999 Dec 23;18(56):7975-84.
Detection limit for recombinant GST tagged MLL4 is approximately 0.1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged MLL4 is approximately 0.03ng/ml as a capture antibody.
Human HepG2; WB Suggested Anti-MLL4 Antibody Titration: 5ug/ml. Positive Control: HepG2 cell lysate. KMT2B is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; MLL4 antibody - N-terminal region (ARP33704_T100) in Human HepG2 cells using Western Blot
Western blot was performed on whole cell lysates from mouse embryonic stem cells ( E14Tg2a ) with Mll4 polyclonal antibody ( Cat # PAB14067 ), diluted 1 : 500 in PBS-Tween containing 5% skimmed milk.Cells homozygous for the targeted mll4 allele ( -/-, Lane 2 and 3 ) show a complete loss of Mll4 protein, whereas the Mll4 protein was detected in flp-recombined ES cells ( F/F, Lane 4 ) and heterozygously targeted ES cells ( +/-, Lane 1 ).
ELISA was performed using a serial dilution of Mll4 polyclonal antibody ( Cat # PAB14067 ). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 15,000.
Human Testis (formalin-fixed, paraffin-embedded) stained with MLL4 antibody AP22567PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with MLL4 antibody AP22567PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis of MLL4 expression in HL60 (A); A549 (B); NIH3T3 (C); H9C2 (D) whole cell lysates.
(5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA311425 | MLL4 (C-term) antibody |
||||||
| Goat Anti-MLL4 Antibody | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt | E, P |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA347232 | MLL4 antibody |
||||||
| Rabbit Polyclonal Mll2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Ms | E |
0.1 ml /
€285.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP22567PU-N | MLL4 (2703-2715) antibody |
||||||
 |
Goat | Aff - Purified | Bov, Hu, Mky, Ms, Por, Rt, Ze | E, P |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||