MLTK antibody
Principal name
MLTK antibody
Alternative names for MLTK antibody
ZAK, HCCS4, HCCS-4, MLKLAK, Mitogen-activated protein kinase kinase kinase MLT, MLK-like mitogen-activated protein triple kinase, Cervical cancer suppressor gene 4 protein
SwissProt ID
Q9NYL2 (Human)
Gene ID
51776 (ZAK)
Ncbi ID
9606, NP_057737.2, NP_598407.1, NM_016653, NP_598407, NP_057737
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of MLTK antibody
- Kit Component:
- - KN200069G1, ZAK gRNA vector 1 in pCas-Guide vector
- KN200069G2, ZAK gRNA vector 2 in pCas-Guide vector
- KN200069D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Detection limit for recombinant GST tagged ZAK is approximately 10ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to ZAK on HeLa cell. [antibody concentration 10 ug/ml]
Detection limit for recombinant GST tagged ZAK is approximately 0.3ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to ZAK on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]
ZAK monoclonal antibody (M03), clone 3G5. Western Blot analysis of ZAK expression in human stomach.
Detection limit for recombinant GST tagged ZAK is approximately 0.03ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MLKLAK polyclonal antibody ( Cat # PAB3277 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Western blot analysis of MLKLAK polyclonal antibody ( Cat # PAB3277 ) in HepG2 cell line lysate ( 35 µg/lane ) . MLKLAK ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . This western blot detected isoform 2 of MLKLAK, the accession number for MLKLAK isoform 1 is Q9NYL2, NP_057737.
ZAK monoclonal antibody (M03), clone 3G5. Western Blot analysis of ZAK expression in human stomach.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Western blot analysis of anti-MLKLAK-C284 Pab in HepG2 cell line lysate (35ug/lane). MLKLAK-C284(arrow) was detected using the purified Pab (1:60 dilution). This western blot detected isoform 2 of MLKLAK.
Formalin-Fixed, Paraffin-Embedded Human Skin stained with ZAk Antibody Cat.-No AP55677PU-N
Immunofluorescent analysis of ZAK staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of ZAK expression in HeLa (A); HepG2 (B); NIH3T3 (C) whole cell lysates.
Western blot analysis of extracts from Jurkat cells, using ZAK (Ab-165) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HeLa cells, using MLTK antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using MLTK antibody.The picture on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315502 | MLTK antibody |
||||||
| Rabbit polyclonal ZAK (Ab-165) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA315503 | MLTK antibody |
||||||
| Rabbit polyclonal anti-MLTK (ZAK/AZK) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| AP15015PU-N | MLTK (Center) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP55677PU-N | MLTK antibody |
||||||
 |
Rabbit | Aff - Purified | Hu | P, WB |
50 µl /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300069 | MLTK (transcript variant 2) |
||||
| Recombinant protein of human sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP321970 | MLTK (transcript variant 1) |
||||
| Recombinant protein of human sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY413852 | ZAK overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. | ||
| LY429511 | ZAK overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |