MMAA antibody
Principal name
MMAA antibody
Alternative names for MMAA antibody
Methylmalonic aciduria type A, MGC120010, MGC120011, MGC120012, Methylmalonic aciduria type A protein, mitochondrial
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of MMAA antibody
- Kit Component:
- - KN223205G1, MMAA gRNA vector 1 in pCas-Guide vector
- KN223205G2, MMAA gRNA vector 2 in pCas-Guide vector
- KN223205D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Honjo, R.S., et al. Genet Test Mol Biomarkers 13(2):181-183(2009)
Merinero, B., et al. J. Inherit. Metab. Dis. 31(1):55-66(2008)
Horster, F., et al. Pediatr. Res. 62(2):225-230(2007)
Padovani, D., et al. J. Biol. Chem. 281(26):17838-17844(2006)
Lerner-Ellis, J.P., et al. Hum. Mutat. 24(6):509-516(2004)
Western Blot analysis of MMAA expression in transfected 293T cell line (H00166785-T02) by MMAA MaxPab polyclonal antibody.Lane 1: MMAA transfected lysate(45.98 KDa).Lane 2: Non-transfected lysate.
Immunohostochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle tissue reacted with MMAA Antibody (N-term) Cat.-No AP52710PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of MMAA Antibody (N-term) Cat.-No AP52710PU-N in mouse liver tissue lysates (35ug/lane). MMAA (arrow) was detected using the purified Pab
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MMAA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502277)
Anti-MMAA mouse monoclonal antibody (TA502277) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMAA(RC223205).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMAA (RC223205, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMAA.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MMAA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502278)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMAA (RC223205, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMAA.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MMAA monoclonal antibody.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502277 | MMAA antibody |
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| MMAA mouse monoclonal antibody, clone OTI3G5 (formerly 3G5) | |||||||
 |
Mouse | IgG2a | OTI3G5 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|
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| TA502278 | MMAA antibody |
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| MMAA mouse monoclonal antibody, clone OTI3A3 (formerly 3A3) | |||||||
 |
Mouse | IgG1 | OTI3A3 | Purified | Hu | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP52710PU-N | MMAA (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP323205 | MMAA |
||||
| Recombinant protein of human methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), nuclear gene encoding mitochondrial protein | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403540 | MMAA overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206557L2V | Lenti ORF particles, Mmaa (GFP-tagged) - Mouse methylmalonic aciduria (cobalamin deficiency) type A (Mmaa), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR206557L1V | Lenti ORF particles, Mmaa (Myc-DDK-tagged) - Mouse methylmalonic aciduria (cobalamin deficiency) type A (Mmaa), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
