MMACHC antibody
Principal name
MMACHC antibody
Alternative names for MMACHC antibody
Methylmalonic aciduria and homocystinuria type C, DKFZp564I122, FLJ25671, RP11-291L19.3, cobalamin deficiency
Gene ID
25974 (MMACHC)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of MMACHC antibody
- Kit Component:
- - KN223846G1, MMACHC gRNA vector 1 in pCas-Guide vector
- KN223846G2, MMACHC gRNA vector 2 in pCas-Guide vector
- KN223846D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Lerner-Ellis JP.,et al. (2006) Nat Genet. 38(1):93-100.Tsai AC.,et al. (2007) Am. J. Med. Genet. A 143A (20): 2430-4.
Western Blot analysis of MMACHC expression in transfected 293T cell line by MMACHC MaxPab polyclonal antibody.Lane 1: MMACHC transfected lysate(24.75 KDa).Lane 2: Non-transfected lysate.
MMACHC MaxPab rabbit polyclonal antibody. Western Blot analysis of MMACHC expression in human liver.
Western Blot analysis of MMACHC expression in transfected 293T cell line (H00025974-T02) by MMACHC MaxPab polyclonal antibody.Lane 1: MMACHC transfected lysate(25.30 KDa).Lane 2: Non-transfected lysate.
Human U937; WB Suggested Anti-MMACHC Antibody. . Titration: 1.0 ug/ml. . Positive Control: U937 Whole Cell; MMACHC antibody - C-terminal region (ARP61734_P050) in Human U937 cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC.
HEK293T cells transfected with either RC223846 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MMACHC antibody(TA504343), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC.
HEK293T cells transfected with either RC223846 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MMACHC antibody(TA504934), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC.
HEK293T cells transfected with either RC223846 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MMACHC antibody(TA504935), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC.
HEK293T cells transfected with either RC223846 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MMACHC antibody(TA504939), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MMACHC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504954)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MMACHC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504954)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MMACHC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504954)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MMACHC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504954)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MMACHC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504954)
Anti-MMACHC mouse monoclonal antibody (TA504954) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMACHC(RC223846).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC.
HEK293T cells transfected with either RC223846 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MMACHC antibody(TA504954), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC.
HEK293T cells transfected with either RC223846 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MMACHC antibody(TA504955), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC.
HEK293T cells transfected with either RC223846 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MMACHC antibody(TA504998), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC (RC223846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC.
HEK293T cells transfected with either RC223846 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MMACHC antibody(TA505081), and then analyzed by flow cytometry.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged MMACHC or untagged Kv2.1 plasmid and probed with N230/21 (left) and N52A/42 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged MMACHC or untagged Kv2.1 plasmid and probed with N230/21 (left) and N52A/42 (right) TC supe.
Sample (50 ug of whole cell lysate). A: Mouse brain. 12% SDS PAGE. TA308065 diluted at 1:1000.
Sample(30 ug whole cell lysate). A:293T. B:Raji . 12% SDS PAGE. TA308065 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using MMACHC(TA308065) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Hepatoma, using MMACHC(TA308065) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MMACHC(TA308065) antibody at 1:100 dilution.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308065 | MMACHC antibody |
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| Rabbit Polyclonal antibody to MMACHC (methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA504954 | MMACHC antibody |
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| MMACHC mouse monoclonal antibody, clone OTI1A4 (formerly 1A4) | |||||||
 |
Mouse | IgG1 | OTI1A4 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| TA504343 | MMACHC antibody |
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| MMACHC mouse monoclonal antibody, clone OTI4E9 (formerly 4E9) | |||||||
 |
Mouse | IgG1 | OTI4E9 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA504934 | MMACHC antibody |
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| MMACHC mouse monoclonal antibody, clone OTI2A6 (formerly 2A6) | |||||||
 |
Mouse | IgG1 | OTI2A6 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA504935 | MMACHC antibody |
||||||
| MMACHC mouse monoclonal antibody, clone OTI1B7 (formerly 1B7) | |||||||
 |
Mouse | IgG1 | OTI1B7 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA504939 | MMACHC antibody |
||||||
| MMACHC mouse monoclonal antibody, clone OTI1E7 (formerly 1E7) | |||||||
 |
Mouse | IgG2a | OTI1E7 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA504955 | MMACHC antibody |
||||||
| MMACHC mouse monoclonal antibody, clone OTI6H5 (formerly 6H5) | |||||||
 |
Mouse | IgG1 | OTI6H5 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA504998 | MMACHC antibody |
||||||
| MMACHC mouse monoclonal antibody, clone OTI4E12 (formerly 4E12) | |||||||
 |
Mouse | IgG1 | OTI4E12 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA505081 | MMACHC antibody |
||||||
| MMACHC mouse monoclonal antibody, clone OTI6B5 (formerly 6B5) | |||||||
 |
Mouse | IgG2b | OTI6B5 | Purified | Hu | F, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| 73-239 | MMACHC antibody |
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 |
Mouse | IgG1 | N230/21 | Supernatant | Hu, Ms | ICC/IF, WB |
5 ml /
€490.00
|
| Antibodies Incorporated | |||||||
|
|||||||
| 75-239 | MMACHC antibody |
||||||
 |
Mouse | IgG1 | N230/21 | Purified | Hu, Ms | ICC/IF, WB |
0.1 ml /
€570.00
|
| Antibodies Incorporated | |||||||
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP323846 | MMACHC |
||||
| Recombinant protein of human methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria (MMACHC) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402444 | MMACHC overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |