MPI antibody
Principal name
MPI antibody
Alternative names for MPI antibody
Mannose-6-phosphate isomerase, Phosphomannose isomerase, Phosphohexomutase, PMI1
Ncbi ID
9606, NP_010918, NP_002426, NP_002426.1, NM_002435, NM_001004081
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Dot blot (Dot), Enzyme Immunoassay (E), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Radioimmunoassay (R), Flow Cytometry (F), Paraffin Sections (P)
Background of MPI antibody
- Kit Component:
- - KN208134G1, MPI gRNA vector 1 in pCas-Guide vector
- KN208134G2, MPI gRNA vector 2 in pCas-Guide vector
- KN208134D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Schollen E., et al. (2000) Hum Mutat. 16:247-252.
Western Blot analysis of MPI expression in transfected 293T cell line (H00004351-T01) by MPI MaxPab polyclonal antibody.Lane 1: MPI transfected lysate(46.53 KDa).Lane 2: Non-transfected lysate.
Recombinant human MPI, 1-362aa, His-tagged
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504749)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504749)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504749)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504749)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504749)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504749)
Anti-MPI mouse monoclonal antibody (TA504749) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MPI(RC208134).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MPI (RC208134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MPI.
HEK293T cells transfected with either RC208134 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MPI antibody(TA504749), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MPI (RC208134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MPI.
HEK293T cells transfected with either RC208134 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MPI antibody(TA504771), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504772)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504772)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504772)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504772)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504772)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504772)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504772)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504772)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504772)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504772)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504772)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504772)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MPI (RC208134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MPI.
Flow cytometric Analysis of Hela cells, using anti-MPI antibody(TA504772),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504792)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504792)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504792)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504792)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504792)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504792)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504792)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MPI (RC208134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MPI.
HEK293T cells transfected with either RC208134 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MPI antibody(TA504792), and then analyzed by flow cytometry.
Sample(30ug whole cell lysate). A: 293T. B: A431 . C: H1299. D: HeLa S3 . E: Hep G2 . F: MOLT4 . G: Raji . 10% SDS PAGE. TA307934 diluted at 1:1000
MPI antibody detects MPI protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 10 % SDS-PAGE. MPI antibody (TA307934) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded Gastric CA N87 xenograft, using MPI(TA307934) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate). . A: NT2D1. . 10% SDS PAGE. . TA308211 diluted at 1:1000.
Western blot detection of Mannose Phosphate Isomerase in Rat kidney,Rat brain,A549 and Lncap cell lysates and using Mannose Phosphate Isomerase mouse mAb (1:1000 diluted).Predicted band size: 54KDa.Observed band size: 45KDa.
Immunocytochemistry stain of Hela using Mannose Phosphate Isomerase mouse mAb (1:300).
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA504749 | MPI antibody |
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| MPI mouse monoclonal antibody, clone OTI1C7 (formerly 1C7) | |||||||
 |
Mouse | IgG1 | OTI1C7 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +8 additional images |
Highly validated antibody!
|
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| TA504772 | MPI antibody |
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| MPI mouse monoclonal antibody, clone OTI4G7 (formerly 4G7) | |||||||
 |
Mouse | IgG1 | OTI4G7 | Purified | Hu | F, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +13 additional images |
Highly validated antibody!
|
||||||
| TA504792 | MPI antibody |
||||||
| MPI mouse monoclonal antibody, clone OTI4E7 (formerly 4E7) | |||||||
 |
Mouse | IgG1 | OTI4E7 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
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| TA307934 | MPI antibody |
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| Rabbit polyclonal antibody to MPI (mannose phosphate isomerase) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Rt | P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA308211 | MPI antibody |
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| Rabbit polyclonal antibody to MPI (mannose phosphate isomerase) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347004 | MPI antibody |
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| Mouse Monoclonal Mannose Phosphate Isomerase Antibody | |||||||
 |
Mouse | IgG1 | 4G9-B4-B8 | Purified | ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA504771 | MPI antibody |
||||||
| MPI mouse monoclonal antibody, clone OTI4A11 (formerly 4A11) | |||||||
 |
Mouse | IgG1 | OTI4A11 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308134 | MPI |
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| Recombinant protein of human mannose phosphate isomerase (MPI) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419324 | MPI overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201799L2V | Lenti ORF particles, Mpi (GFP-tagged ORF) - Rat mannose phosphate isomerase (mapped) (Mpi), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR201799L1V | Lenti ORF particles, Mpi (Myc-DDK-tagged ORF) - Rat mannose phosphate isomerase (mapped) (Mpi), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||