MRAP antibody
Principal name
MRAP antibody
Alternative names for MRAP antibody
Melanocortin-2 receptor accessory protein, C21orf61, FALP, Fat cell-specific low molecular weight protein, Fat tissue-specific low MW protein, B27
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Flow Cytometry (F), Paraffin Sections (P)
Background of MRAP antibody
- Kit Component:
- - KN209377G1, MRAP gRNA vector 1 in pCas-Guide vector
- KN209377G2, MRAP gRNA vector 2 in pCas-Guide vector
- KN209377D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Dias, R.P. et al., Eur. J. Endocrinol. 162(2): 357-359 (2010)
Chan, L.F. et al., Proc. Natl. Acad. Sci. U.S.A. 106(15): 6146-6151 (2009)
Webb, T.R. et al., Endocrinology 150(2): 720-726 (2009)
Western Blot analysis of MRAP expression in transfected 293T cell line (H00056246-T01) by MRAP MaxPab polyclonal antibody.Lane 1: MRAP transfected lysate(18.92 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MRAP expression in transfected 293T cell line (H00056246-T02) by MRAP MaxPab polyclonal antibody.Lane 1: MRAP transfected lysate(18.92 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry analysis in breast carcinoma (Formalin-fixed, Paraffin-embedded) using MRAP antibody Cat.-No. AP52742PU-N (N-term), followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated.
Western blot analysis of MRAP (arrow) in 293 cell line lysates (35ug/lane) using MRAP antibody Cat.-No. AP52742PU-N (N-term).
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram) using MRAP antibody Cat.-No. AP52742PU-N (N-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP52742PU-N | MRAP (N-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP318683 | MRAP (transcript variant 2) |
||||
| Purified recombinant protein of Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY404158 | MRAP overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR200684L2V | Lenti ORF particles, Mrap (GFP-tagged) - Mouse melanocortin 2 receptor accessory protein (Mrap), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR200684L1V | Lenti ORF particles, Mrap (Myc-DDK-tagged) - Mouse melanocortin 2 receptor accessory protein (Mrap), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||