MRI1 antibody
Principal name
MRI1 antibody
Alternative names for MRI1 antibody
MTR-1-P isomerase, M1Pi, Methylthioribose-1-phosphate isomerase, S-methyl-5-thioribose-1-phosphate isomerase, Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein
Ncbi ID
9606, NP_080699, NM_032285, NP_001026897.1, NM_001031727, NP_001026897, NP_080699.3, NM_026423, NM_001010947
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)
Background of MRI1 antibody
- Kit Component:
- - KN310307G1, 2410018C20Rik gRNA vector 1 in pCas-Guide vector
- KN310307G2, 2410018C20Rik gRNA vector 2 in pCas-Guide vector
- KN310307D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Katayama,S., et al., (2005) Science 309 (5740), 1564-1566
MGC3207 monoclonal antibody (M01), clone 2D11-1G4 Western Blot analysis of MGC3207 expression in HL-60 ( Cat # L014V1 ).
Detection limit for recombinant GST tagged MGC3207 is approximately 0.1ng/ml as a capture antibody.
Mouse NIH-3T3; WB Suggested Anti-Mri1 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:1562500. Positive Control: NIH/3T3 cell lysate; Mri1 antibody - N-terminal region (ARP37364_T100) in Mouse NIH-3T3 cells using Western Blot
Mouse NIH-3T3; WB Suggested Anti-Mri1 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:1562500. Positive Control: NIH/3T3 cell lysate; Mri1 antibody - C-terminal region (ARP37363_T100) in Mouse NIH-3T3 cells using Western Blot
Western Blot analysis of MGC3207 expression in transfected 293T cell line (H00084245-T01) by MGC3207 MaxPab polyclonal antibody.Lane 1: MGC3207 transfected lysate(40.59 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MGC3207 expression in transfected 293T cell line (H00084245-T02) by MGC3207 MaxPab polyclonal antibody.Lane 1: MGC3207 transfected lysate(39.10 KDa).Lane 2: Non-transfected lysate.
Human MCF-7; WB Suggested Anti-MGC3207 Antibody. . Titration: 1.0 ug/ml. . Positive Control: MCF7 Whole Cell; MGC3207 antibody - N-terminal region (ARP60474_P050) in Human MCF-7 cells using Western Blot
Human Fetal liver; WB Suggested Anti-MGC3207 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Liver; MGC3207 antibody - N-terminal region (ARP60475_P050) in Human Fetal liver cells using Western Blot
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500433)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500433)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500433)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500433)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500433)
Anti-MRI1 mouse monoclonal antibody (TA500433) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MRI1(RC200993).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MRI1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRI1 (RC200993, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRI1.
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500490)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500490)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500490)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500490)
Immunofluorescent staining of A549 cells using anti-MRI1 mouse monoclonal antibody (TA500490).
Anti-MRI1 mouse monoclonal antibody (TA500490) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MRI1(RC200993).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRI1 (RC200993, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRI1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MRI1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500491)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500491)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500491)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500491)
Anti-MRI1 mouse monoclonal antibody (TA500491) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MRI1(RC200993).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRI1 (RC200993, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRI1.
HEK293T cells transfected with either RC200993 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MRI1 antibody(TA500491), and then analyzed by flow cytometry.
WB Suggested Anti-Mri1 Antibody Titration: 2.5ug/ml; ELISA Titer: 1:1562500; Positive Control: NIH/3T3 cell lysate
WB Suggested Anti-Mri1 Antibody Titration: 2.5ug/ml; ELISA Titer: 1:1562500; Positive Control: NIH/3T3 cell lysate
WB Suggested Anti-MGC3207 Antibody; Titration: 1.0 ug/ml; Positive Control: MCF7 Whole Cell
WB Suggested Anti-MGC3207 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Liver
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500433 | MRI1 antibody |
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| MRI1 mouse monoclonal antibody, clone OTI6G9 (formerly 6G9) | |||||||
 |
Mouse | IgG1 | OTI6G9 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +7 additional images |
Highly validated antibody!
|
||||||
| TA500490 | MRI1 antibody |
||||||
| MRI1 mouse monoclonal antibody, clone OTI6A10 (formerly 6A10) | |||||||
 |
Mouse | IgG1 | OTI6A10 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
||||||
| TA500491 | MRI1 antibody |
||||||
| MRI1 mouse monoclonal antibody, clone OTI9H7 (formerly 9H7) | |||||||
 |
Mouse | IgG2b | OTI9H7 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300993 | MRI1 (transcript variant 1) |
||||
| Recombinant protein of human methylthioribose-1-phosphate isomerase homolog (S. cerevisiae) (MRI1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY422176 | MRI1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205693L2V | Lenti ORF particles, Mri1 (GFP-tagged) - Mouse methylthioribose-1-phosphate isomerase homolog (S. cerevisiae) (Mri1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205693L1V | Lenti ORF particles, Mri1 (Myc-DDK-tagged) - Mouse methylthioribose-1-phosphate isomerase homolog (S. cerevisiae) (Mri1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR207421L2V | Lenti ORF particles, Mri1 (GFP-tagged ORF) - Rat translation initiation factor eIF-2B subunit alpha/beta/delta-like protein (RGD1307789), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR207421L1V | Lenti ORF particles, Mri1 (Myc-DDK-tagged ORF) - Rat translation initiation factor eIF-2B subunit alpha/beta/delta-like protein (RGD1307789), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||