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MRPL10 antibody

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Principal name

MRPL10 antibody

Alternative names for MRPL10 antibody

L10mt, MRP-L10, MRPL8, RPML8, 39S ribosomal protein L10, mitochondrial

SwissProt ID

P0C2C4 (Rat), P36520 (Yeast), Q3MHY7 (Bovin), Q3TBW2 (Mouse), Q7Z7H8 (Human)

Gene ID

124995 (MRPL10), 107732 (Mrpl10)

Ncbi ID

9606, NP_660298, NP_660298.2, NM_145255, NP_080430.1, NM_148887

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P)

Background of MRPL10 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 5q. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 5q.

General readings

Wang,C.C., (2004) Biochem. Biophys. Res. Commun. 314 (2), 335-350

H00124995-B01 - MRPL10
Western Blot analysis of MRPL10 expression in transfected 293T cell line (H00124995-T01) by MRPL10 MaxPab polyclonal antibody.Lane 1: MRPL10 transfected lysate(28.82 KDa).Lane 2: Non-transfected lysate.
H00124995-B01P - MRPL10
Western Blot analysis of MRPL10 expression in transfected 293T cell line (H00124995-T01) by MRPL10 MaxPab polyclonal antibody.Lane 1: MRPL10 transfected lysate(28.82 KDa).Lane 2: Non-transfected lysate.
H00124995-B02 - MRPL10
Western Blot analysis of MRPL10 expression in transfected 293T cell line (H00124995-T02) by MRPL10 MaxPab polyclonal antibody.Lane 1: MRPL10 transfected lysate(28.71 KDa).Lane 2: Non-transfected lysate.
H00124995-B02P - MRPL10
Western Blot analysis of MRPL10 expression in transfected 293T cell line (H00124995-T03) by MRPL10 MaxPab polyclonal antibody.Lane 1: MRPL10 transfected lysate(28.71 KDa).Lane 2: Non-transfected lysate.
ARP52830_P050 - MRPL10
Human Jurkat; WB Suggested Anti-MRPL10 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; MRPL10 antibody - N-terminal region (ARP52830_P050) in Human Jurkat cells using Western Blot
C14054-1 - MRPL10
Western blot analysis of extracts from 293/HepG2/COLO cells, using MRPL10 Antibody. The lane on the right is treated with the synthesized peptide.
CPA2853-100ul - MRPL10
Immunohistochemical analysis of MRPL10 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA2853-100ul - MRPL10
Western blot analysis of MRPL10 expression in HT29 (A); Jurkat (B); HepG2 (C) whole cell lysates.
TA808643 - MRPL10
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MRPL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808643)(1:150)
TA808643 - MRPL10
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MRPL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808643)(1:150)
TA808643 - MRPL10
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MRPL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808643)(1:150)
TA808643 - MRPL10
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MRPL10 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808643)(1:150)
TA808643 - MRPL10
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MRPL10 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808643)(1:150)
TA808643 - MRPL10
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MRPL10 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808643)(1:150)
TA808643 - MRPL10
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MRPL10 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808643)(1:150)
TA808643 - MRPL10
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MRPL10 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808643)(1:150)
TA808643 - MRPL10
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MRPL10 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808643)(1:150)
TA808643 - MRPL10
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPL10 (RC208608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPL10.(1:2000)
TP760175 - MRPL10
TA316074 - MRPL10
Western blot analysis of extracts from HepG2 cells, COLO cells and 293 cells, using MRPL10 antibody.The lane on the right is treated with the synthesized peptide.
TA339305 - MRPL10
Host: Rabbit; Target Name: MRPL10; Sample Tissue: HepG2 Whole cell lysates; Antibody Dilution: 1.0 ug/ml
TA340398 - MRPL10
WB Suggested Anti-MRPL10 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MRPL10 antibody
  MRPL10 mouse monoclonal antibody, clone OTI2B4 (formerly 2B4)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MRPL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808643)(1:150) Mouse IgG2b OTI2B4 Purified Hu P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+9 additional images

Highly validated antibody!

TrueMAB

MRPL10 antibody
  Rabbit polyclonal anti-MRPL10 antibody  
Western blot analysis of extracts from HepG2 cells, COLO cells and 293 cells, using MRPL10 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MRPL10 (full length, N-term HIS tag)
  Recombinant protein of human mitochondrial ribosomal protein L10 (MRPL10), nuclear gene encoding mitochondrial protein, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
MRPL10 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

MRPL10 overexpression lysate
MRPL10 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

4 Item(s)

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Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (2)
  3. Protein | Growth Factor (1)
Clonality
  1. Monoclonal (1)
  2. Polyclonal (1)
Target
  1. MRPL10 (3)
Reactivity
  1. Human (Hu) (2)
Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Enzyme Immunoassay (E) (1)
  3. Paraffin Sections (P) (1)
Presentation
  1. Purified (2)
Host
  1. Mouse (1)
  2. Rabbit (1)
Images
  1. 1 image (3)
  2. 3+ images (1)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. E. coli (1)
Manufacturer
  1. OriGene Technologies (4)
Service
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