MRPL12 antibody
Principal name
MRPL12 antibody
Alternative names for MRPL12 antibody
L12mt, MRP-L12, RPML12, 5c5-2, 39S ribosomal protein L12, mitochondrial
Ncbi ID
9606, NP_002940.2, AAH02344, NM_002949, NP_002940, NP_081480.2, NM_027204
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of MRPL12 antibody
- Kit Component:
- - KN310321G1, Mrpl12 gRNA vector 1 in pCas-Guide vector
- KN310321G2, Mrpl12 gRNA vector 2 in pCas-Guide vector
- KN310321D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 3ug/ml]
Immunofluorescence of monoclonal antibody to MRPL12 on HeLa cell. [antibody concentration 10 ug/ml]
Western blot analysis of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi ( Cat # H00006182-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MRPL12 monoclonal antibody (M01), clone 3B12-1A3 (Cat # H00006182-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody (M01), clone 3B12-1A3.Lane 1: MRPL12 transfected lysate(21.3 KDa).Lane 2: Non-transfected lysate.
MRPL12 monoclonal antibody (M01), clone 3B12-1A3 Western Blot analysis of MRPL12 expression in COLO 320 HSR ( Cat # L020V1 ).
Detection limit for recombinant GST tagged MRPL12 is approximately 0.03ng/ml as a capture antibody.
Western Blot analysis of MRPL12 expression in transfected 293T cell line (H00006182-T01) by MRPL12 MaxPab polyclonal antibody.Lane 1: MRPL12 transfected lysate(21.30 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of MRPL12 expression in HeLa (A); SP2/0 (B); H9C2 (C) whole cell lysates.
Western blot analysis of extracts from COLO cells, using MRPL12 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-MRPL12 Antibody; Titration: 1.0 ug/ml; Positive Control: U937 Whole Cell
Host: Rabbit; Target Name: Mrpl12; Sample Tissue: Rat Lung lysates; Antibody Dilution: 1.0 ug/ml
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: 293T cells, Primary antibody: (MRPL12 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (MRPL12 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (MRPL12 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314473 | MRPL12 antibody |
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| Rabbit polyclonal anti-MRPL12 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA350182 | MRPL12 antibody |
||||||
| Rabbit Polyclonal Anti-MRPL12 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301832 | MRPL12 |
||||
| Recombinant protein of human mitochondrial ribosomal protein L12 (MRPL12), nuclear gene encoding mitochondrial protein | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY418996 | MRPL12 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR202047L2V | Lenti ORF particles, Mrpl12 (GFP-tagged) - Mouse mitochondrial ribosomal protein L12 (Mrpl12), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR202047L1V | Lenti ORF particles, Mrpl12 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L12 (Mrpl12), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||