MRPL15 antibody
Principal name
MRPL15 antibody
Alternative names for MRPL15 antibody
L15mt, MRP-L15, HSPC145, 39S ribosomal protein L15, mitochondrial
SwissProt ID
P36523 (Yeast), Q0VC21 (Bovin), Q5ZKT8 (Chick), Q6DGM3 (Danre), Q9CPR5 (Mouse), Q9P015 (Human)
Ncbi ID
9606, NP_054894, NP_054894.1, NM_014175, NP_001171129.1, NM_025300, NM_001177658
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)
Background of MRPL15 antibody
- Kit Component:
- - KN310324G1, Mrpl15 gRNA vector 1 in pCas-Guide vector
- KN310324G2, Mrpl15 gRNA vector 2 in pCas-Guide vector
- KN310324D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of MRPL15 expression in transfected 293T cell line (H00029088-T01) by MRPL15 MaxPab polyclonal antibody.Lane 1: MRPL15 transfected lysate(32.56 KDa).Lane 2: Non-transfected lysate.
Human PANC1; WB Suggested Anti-MRPL15 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: PANC1 cell lysate. MRPL15 is supported by BioGPS gene expression data to be expressed in PANC1.; MRPL15 antibody - N-terminal region (ARP46191_P050) in Human PANC1 cells using Western Blot
Human MCF-7; WB Suggested Anti-MRPL15 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate. MRPL15 is supported by BioGPS gene expression data to be expressed in MCF7.; MRPL15 antibody - C-terminal region (ARP46192_P050) in Human MCF-7 cells using Western Blot
Immunohistochemical analysis of MRPL15 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of MRPL15 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of MRPL15 expression in PANC1 (A); HuvEc (B) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MRPL15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807342)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MRPL15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807342)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPL15 (RC201471, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPL15.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPL15 (RC201471, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPL15.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPL15 (RC201471, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPL15.
Western blot analysis of extracts from HUVEC cells, HeLa cells and MCF-7cells, using MRPL15 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-MRPL15 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: PANC1 cell lysate.MRPL15 is supported by BioGPS gene expression data to be expressed in PANC1
WB Suggested Anti-MRPL15 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: MCF7 cell lysate.MRPL15 is supported by BioGPS gene expression data to be expressed in MCF7
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315393 | MRPL15 antibody |
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| Rabbit polyclonal anti-MRPL15 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA807342 | MRPL15 antibody |
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| MRPL15 mouse monoclonal antibody, clone OTI7A3 (formerly 7A3) | |||||||
 |
Mouse | IgG2b | OTI7A3 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA807409 | MRPL15 antibody |
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| MRPL15 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3) | |||||||
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Mouse | IgG2a | OTI2D3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA807729 | MRPL15 antibody |
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| MRPL15 mouse monoclonal antibody, clone OTI10D3 (formerly 10D3) | |||||||
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Mouse | IgG1 | OTI10D3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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