MRPL28 antibody
Principal name
MRPL28 antibody
Alternative names for MRPL28 antibody
L28mt, MAAT1, MRP-L28, Melanoma antigen p15, 39S ribosomal protein L28, mitochondrial, Melanoma-associated antigen recognized by T lymphocytes
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of MRPL28 antibody
- Kit Component:
- - KN201794G1, MRPL28 gRNA vector 1 in pCas-Guide vector
- KN201794G2, MRPL28 gRNA vector 2 in pCas-Guide vector
- KN201794D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Koc EC, et al. (2001) J Biol Chem 276 (47): 43958–69.Kawakami, Y., et al. (2000) J.Immunother. 23: 17-27.
Western Blot analysis of MRPL28 expression in transfected 293T cell line (H00010573-T01) by MRPL28 MaxPab polyclonal antibody.Lane 1: MRPL28 transfected lysate(14.90 KDa).Lane 2: Non-transfected lysate.
MRPL28 MaxPab polyclonal antibody. Western Blot analysis of MRPL28 expression in human placenta.
Western Blot analysis of MRPL28 expression in transfected 293T cell line (H00010573-T02) by MRPL28 MaxPab polyclonal antibody.Lane 1: MRPL28 transfected lysate(28.16 KDa).Lane 2: Non-transfected lysate.
Human PANC1; WB Suggested Anti-MRPL28 Antibody Titration: 0.2-1 ug/ml. Positive Control: PANC1 cell lysate. MRPL28 is supported by BioGPS gene expression data to be expressed in PANC1.; MRPL28 antibody - middle region (ARP52182_P050) in Human PANC1 cells using Western Blot
Western blot analysis of MRPL28 expression in JAR (A); MCF7 (B); A549 (C); Raw264.7 (D); H9C2 (E) whole cell lysates.
MRPL28 Antibody (N-term) (Cat. #TA324663) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MRPL28 antibody detected the MRPL28 protein (arrow).
IF image of A431 cell stained with MRPL28 Antibody (N-term)(Cat#TA324663).A431 cells were incubated with MRPL28 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . MRPL28 immunoreactivity is localized to Mitochondria significantly.
Immunofluorescence analysis of HeLa cell using MRPL28 antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-MRPL28 Antibody Titration: 0.2-1 ug/ml; Positive Control: PANC1 cell lysateMRPL28 is supported by BioGPS gene expression data to be expressed in PANC1
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: 293T cells, Primary antibody: (MRPL28 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: 293T cells, human testis tissue, A375 cells, human normal kidney tissue, Primary antibody: (MRPL28 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (MRPL28 Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (MRPL28 Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324663 | MRPL28 antibody |
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| Rabbit polyclonal MRPL28 Antibody (N-term) | |||||||
 |
Rabbit | IgG | Purified | Hu | ICC/IF, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA332976 | MRPL28 antibody |
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| Rabbit anti-MRPL28 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA350183 | MRPL28 antibody |
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| Rabbit Polyclonal Anti-MRPL28 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA350714 | MRPL28 antibody |
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| Rabbit Polyclonal Anti-MRPL28 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301794 | MRPL28 |
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| Recombinant protein of human mitochondrial ribosomal protein L28 (MRPL28), nuclear gene encoding mitochondrial protein | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416653 | MRPL28 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |