MRPL50 antibody
Principal name
MRPL50 antibody
Alternative names for MRPL50 antibody
L50mt, MRP-L50, 39S ribosomal protein L50, mitochondrial
Ncbi ID
9606, NP_061924.1, NP_061924, NM_019051, NP_848718.1, NM_178603
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of MRPL50 antibody
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q.
Western Blot analysis of MRPL50 expression in transfected 293T cell line (H00054534-T01) by MRPL50 MaxPab polyclonal antibody.Lane 1: MRPL50 transfected lysate(17.38 KDa).Lane 2: Non-transfected lysate.
Formalin fixed, paraffin embedded human brain tissue stained with MRPL50 Antibody (C-term) Cat.-No AP53677PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RM50 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of MRPL50 Antibody (C-term) Cat.-No AP53677PU-N in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the RM50 antibody detected the RM50 protein (arrow).
Western blot analysis of extracts from COLO cells and HT-29 cells, using MRPL50 antibody.The lane on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315528 | MRPL50 antibody |
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| Rabbit polyclonal anti-MRPL50 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP53677PU-N | MRPL50 (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760099 | MRPL50 (full length, N-term HIS tag) |
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| Recombinant protein of human mitochondrial ribosomal protein L50 (MRPL50), nuclear gene encoding mitochondrial protein, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY412790 | MRPL50 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201209L2V | Lenti ORF particles, Mrpl50 (GFP-tagged) - Mouse mitochondrial ribosomal protein L50 (Mrpl50), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201209L1V | Lenti ORF particles, Mrpl50 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L50 (Mrpl50), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
