MRPS25 antibody
Principal name
MRPS25 antibody
Alternative names for MRPS25 antibody
S25mt, RPMS25, MRP-S25, 28S ribosomal protein S25, mitochondrial
Ncbi ID
NP_071942, 9606, NP_071942.1, NM_022497, NP_079854.2, NM_001025408, NM_025578
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F)
Background of MRPS25 antibody
- Kit Component:
- - KN310381G1, Mrps25 gRNA vector 1 in pCas-Guide vector
- KN310381G2, Mrps25 gRNA vector 2 in pCas-Guide vector
- KN310381D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Koc EC, Burkhart W, Blackburn K, et al. (2000). J. Biol. Chem. 275 (42): 32585–91.
Zhang Z, Gerstein M (2003). Genomics 81 (5): 468–80.
Cavdar Koc E, et al. (May 2001). J Biol Chem 276 (22): 19363–74.
MRPS25 monoclonal antibody (M01), clone 3E6. Western Blot analysis of MRPS25 expression in NIH/3T3 ( Cat # L018V1 ).
MRPS25 monoclonal antibody (M01), clone 3E6. Western Blot analysis of MRPS25 expression in PC-12(Cat # L012V1 ).
Western Blot analysis of MRPS25 expression in transfected 293T cell line (H00064432-T01) by MRPS25 MaxPab polyclonal antibody.Lane 1: MRPS25 transfected lysate(19.03 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MRPS25 expression in transfected 293T cell line (H00064432-T02) by MRPS25 MaxPab polyclonal antibody.Lane 1: MRPS25 transfected lysate(19.03 KDa).Lane 2: Non-transfected lysate.
Western blot (WB) analysis of MRP-S25 antibody (Cat.-No.: AP21193PU-N) extracts from 293 cells.
Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human lung tissue using RT25 antibody (C-term) Cat.-No AP53758PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RT25 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis in MCF-7 cell line lysates (35 ug/lane) using RT25 Antibody (C-term) Cat.-No AP53758PU-N. RT25 (arrow) was detected using the purified Pab.
Flow Cytometric analysis of MCF-7 cells (bottom histogram) using RT25 Antibody (C-term) Cat.-No AP53758PU-N compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
The cell lysates of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MRPS25 antibody (1:500 ~ 1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis of extracts from 293 cells, using MRPS25 antibody.The lane on the right is treated with the synthesized peptide.
Host: Rabbit; Target Name: MRPS25; Sample Tissue: A549 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: MRPS25; Sample Tissue: U937 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315734 | MRPS25 antibody |
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| Rabbit polyclonal anti-MRPS25 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM50043PU-N | MRPS25 antibody |
||||||
 |
Mouse | IgG2a | AT38E7 | Purified | Hu | E, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
| AM50043PU-S | MRPS25 antibody |
||||||
|
Mouse | IgG2a | AT38E7 | Purified | Hu | E, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| AP21193PU-N | MRPS25 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| AP53758PU-N | MRPS25 (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301353 | MRPS25 |
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| Recombinant protein of human mitochondrial ribosomal protein S25 (MRPS25), nuclear gene encoding mitochondrial protein | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY411645 | MRPS25 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201431L2V | Lenti ORF particles, Mrps25 (GFP-tagged) - Mouse mitochondrial ribosomal protein S25 (Mrps25), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR211861L2V | Lenti ORF particles, Mrps25 (GFP-tagged ORF) - Rat mitochondrial ribosomal protein S25 (Mrps25), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR211861L1V | Lenti ORF particles, Mrps25 (Myc-DDK-tagged ORF) - Rat mitochondrial ribosomal protein S25 (Mrps25), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||