MRPS27 antibody
Principal name
MRPS27 antibody
Alternative names for MRPS27 antibody
MRP-S27, KIAA0264, S27mt, 28S ribosomal protein S27, mitochondria
Ncbi ID
9606, NP_055899, NP_055899.2, NP_776118.2, BC049149, NM_173757, NM_015084
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), EPair for Elisa (EPair), Luminex (LMNX), Flow Cytometry (F), Paraffin Sections (P)
Background of MRPS27 antibody
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein.
Detection limit for recombinant GST tagged MRPS27 is approximately 1ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to MRPS27 on HeLa cell. [antibody concentration 10 ug/ml]
MRPS27 MaxPab polyclonal antibody. Western Blot analysis of MRPS27 expression in human kidney.
Western Blot analysis of MRPS27 expression in transfected 293T cell line (H00023107-T01) by MRPS27 MaxPab polyclonal antibody.Lane 1: MRPS27 transfected lysate(18.48 KDa).Lane 2: Non-transfected lysate.
MRPS27 monoclonal antibody (M14A), clone 3G8 Western Blot analysis of MRPS27 expression in A-431 ( Cat # L015V1 ).
Immunofluorescence of purified MaxPab antibody to MRPS27 on HeLa cell. [antibody concentration 10 ug/ml]
MRPS27 MaxPab polyclonal antibody. Western Blot analysis of MRPS27 expression in human kidney.
Western Blot analysis of MRPS27 expression in transfected 293T cell line (H00023107-T01) by MRPS27 MaxPab polyclonal antibody.Lane 1: MRPS27 transfected lysate(18.48 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MRPS27 expression in transfected 293T cell line (H00023107-T02) by MRPS27 MaxPab polyclonal antibody.Lane 1: MRPS27 transfected lysate(19.00 KDa).Lane 2: Non-transfected lysate.
MRPS27 MaxPab rabbit polyclonal antibody. Western Blot analysis of MRPS27 expression in Jurkat.
Western blot (WB) analysis of MRP-S27 antibody (Cat.-No.: AP21074PU-N) in extracts from HUVEC cells.
Western blot analysis of MRPS27 expression in HeLa (A); A549 (B) whole cell lysates.
MRPS27 Elisa with 3A8 Capture (TA600311) and 6E5 Detection (TA700312) Antibodies. Substrate used: Recombinant Human MRPS27 (LY402416)
MRPS27 Luminex Elisa with 3A8 Capture (TA600311) and 6E5 Detection (TA700312) Antibodies. Substrate used: Recombinant Human MRPS27 (LY402416)
MRPS27 Luminex Elisa with 6E5 Capture (TA600312) and 3A8 Detection (TA700311) Antibodies. Substrate used: Recombinant Human MRPS27 (LY402416)
MRPS27 Luminex Elisa with 6E5 Capture (TA600312) and 3A8 Detection (TA700311) Antibodies. Substrate used: Recombinant Human MRPS27 (LY402416)
MRPS27 ELISA with 3A8 Capture (TA600311) and 6E5 Detection (TA700312) Antibodies. Substrate used: Recombinant Human MRPS27 (LY402416)
MRPS27 Luminex Elisa with 3A8 Capture (TA600311) and 6E5 Detection (TA700312) Antibodies. Substrate used: Recombinant Human MRPS27 (LY402416)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800170)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800170)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800170)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (RC209478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
HEK293T cells transfected with either RC209478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MRPS27 antibody(TA800170), and then analyzed by flow cytometry.
Anti-MRPS27 mouse monoclonal antibody (TA800177) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MRPS27(RC209478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (RC209478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (RC209478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
HEK293T cells transfected with either RC209478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MRPS27 antibody(TA800189), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800198)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (RC209478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MRPS27 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC209478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MRPS27 antibody(TA800198), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800199)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (RC209478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MRPS27 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC209478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MRPS27 antibody(TA800199), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800223)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800223)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (RC209478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
HEK293T cells transfected with either RC209478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MRPS27 antibody(TA800223), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800244)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800244)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800244)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800244)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800244)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800244)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800244)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800244)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800244)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800244)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800244)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800244)
Anti-MRPS27 mouse monoclonal antibody (TA800244) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MRPS27(RC209478).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (RC209478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
HEK293T cells transfected with either RC209478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MRPS27 antibody(TA800244), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800260)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800260)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800260)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800260)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800260)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800260)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800260)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (RC209478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
Western blot analysis of extracts from HeLa cells and HUVEC cells, using MRPS27 antibody.The lane on the right is treated with the synthesized peptide.
- Quick-Filters:
- Highly Validated (6)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA800170 | MRPS27 antibody |
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| MRPS27 mouse monoclonal antibody, clone OTI1A2 (formerly 1A2) | |||||||
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Mouse | IgG1 | OTI1A2 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
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| TA800198 | MRPS27 antibody |
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| MRPS27 mouse monoclonal antibody, clone OTI3D9 (formerly 3D9) | |||||||
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Mouse | IgG2b | OTI3D9 | Purified | Hu, Mky | F, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA800199 | MRPS27 antibody |
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| MRPS27 mouse monoclonal antibody, clone OTI3B9 (formerly 3B9) | |||||||
 |
Mouse | IgG1 | OTI3B9 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA800223 | MRPS27 antibody |
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| MRPS27 mouse monoclonal antibody, clone OTI1A5 (formerly 1A5) | |||||||
 |
Mouse | IgG1 | OTI1A5 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA800244 | MRPS27 antibody |
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| MRPS27 mouse monoclonal antibody, clone OTI6E5 (formerly 6E5) | |||||||
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Mouse | IgG1 | OTI6E5 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
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| TA800260 | MRPS27 antibody |
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| MRPS27 mouse monoclonal antibody, clone OTI3A8 (formerly 3A8) | |||||||
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Mouse | IgG2b | OTI3A8 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| TA312373 | MRPS27 antibody |
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| Rabbit polyclonal anti-MRPS27 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA600311 | MRPS27 antibody |
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| Luminex and ELISA validated MRPS27 mouse monoclonal antibody (clone 3A8, clone OTI3A8 (formerly 3A8) | |||||||
 |
Mouse | IgG1 | OTI3A8 | Purified | Hu | E, LMNX |
0.1 mg /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA600312 | MRPS27 antibody |
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| Luminex validated MRPS27 mouse monoclonal antibody (clone 6E5, clone OTI6E5 (formerly 6E5) | |||||||
 |
Mouse | IgG1 | OTI6E5 | Purified | Hu | LMNX |
0.1 mg /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| TA700311 | MRPS27 antibody |
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| Luminex validated biotinylated MRPS27 mouse monoclonal antibody (clone 3A8), clone OTI3A8 (formerly 3A8) | |||||||
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Mouse | IgG1 | OTI3A8 | Purified | Hu | LMNX |
50 µg /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| TA700312 | MRPS27 antibody |
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| Luminex and ELISA validated biotinylated MRPS27 mouse monoclonal antibody (clone 6E5), clone OTI6E5 (formerly 6E5) | |||||||
 |
Mouse | IgG1 | OTI6E5 | Purified | Hu | E, LMNX |
50 µg /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA800177 | MRPS27 antibody |
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| MRPS27 mouse monoclonal antibody, clone OTI2G9 (formerly 2G9) | |||||||
 |
Mouse | IgG1 | OTI2G9 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA800189 | MRPS27 antibody |
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| MRPS27 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10) | |||||||
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Mouse | IgG1 | OTI2B10 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| AP21074PU-N | MRPS27 antibody |
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Rabbit | Aff - Purified | Hu, Ms | WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760198 | MRPS27 (full length, N-term HIS tag) |
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| Recombinant protein of human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203136L2V | Lenti ORF particles, Mrps27 (GFP-tagged) - Mouse mitochondrial ribosomal protein S27 (cDNA clone MGC:56872 IMAGE:6309524), 200 uL, >10^7 TU/mL |
|
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200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR219080L2V | Lenti ORF particles, Mrps27 (GFP-tagged) - Mouse mitochondrial ribosomal protein S27 (Mrps27), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203136L1V | Lenti ORF particles, Mrps27 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein S27 (cDNA clone MGC:56872 IMAGE:6309524), 200 uL, >10^7 TU/mL |
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200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| MR219080L1V | Lenti ORF particles, Mrps27 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein S27 (Mrps27), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
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200 µl /
€830.00
|
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| OriGene Technologies, Inc. | ||