MTBP antibody
Principal name
MTBP antibody
Alternative names for MTBP antibody
Mdm2-binding protein, hMTBP, Mdm2
Ncbi ID
NP_071328.2, 9606, NP_071328, NM_022045, NP_001161722.1, NM_134092, NM_001168250
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of MTBP antibody
- Kit Component:
- - KN310458G1, Mtbp gRNA vector 1 in pCas-Guide vector
- KN310458G2, Mtbp gRNA vector 2 in pCas-Guide vector
- KN310458D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunocytochemical staining of K562 cells with Rabbit anti Human MTBP (SP1310)
Western blot analysis of MTBP expression in K562 whole cell lysates with SP1310.
Western blot using MTBP monoclonal antibody, clone PHL-1 ( Cat # MAB1995 ) shows detection of aband ~110 kDa corresponding to human MTBP ( arrowhead ).Lanes represent human 293 cell lysates with ( + ) and without ( - ) transfection with a full-length human expression MTBP construct.The transfected cell extract was diluted 30 fold in extract lacking transfected MTBP.Proteins were separated bySDS-PAGE and transferred onto PDVF membrane.After blocking, the membrane was probed with the primary antibody diluted to 1 : 500 for 2h at roomtemperature followed by detection usinga Lumi-Light Plus Western Blotting Kit.
Western blot analysis of MTBP expression in K-562 whole cell lysates with MTBP polyclonal antibody ( Cat # PAB10134 ) at 1 µg /ml.
Western blot using anti-MTBP antibody shows detection of a band ~110 kDa corresponding to human MTBP (arrowhead). Lanes represent human 293 cell lysates with (+) and without (-) transfection with a full-length human expression MTBP construct. The transfected cell extract was diluted 30 fold in extract lacking transfected MTBP. Proteins were separated by SDS-PAGE and transferred onto PDVF membrane. After blocking, the membrane was probed with the primary antibody diluted to 1:500 for 2h at room temperature followed by detection using a Lumi-LightPlus Western Blotting Kit (Roche).
Western blot analysis of MTBP antibody (C-term) (AP17569PU-N) in K562 cell line lysates (35ug/lane). MTBP (arrow) was detected using the purified Pab.
Western blot analysis of MTBP expression in K562 whole cell lysates with MTBP antibody at 1 ug /ml.
Immunocytochemistry of MTBP in K562 cells with MTBP antibody at 5 ug/mL.
Immunofluorescence of MTBP in K562 cells with MTBP antibody at 20 ug/mL.
Host: Rabbit; Target Name: MTBP; Sample Tissue: 293T Whole cell lysates; Antibody Dilution: 1.0 ug/ml
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (MTBP Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306073 | MTBP (Center) antibody |
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| Rabbit Polyclonal MTBP Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | E, ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA351414 | MTBP antibody |
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| Rabbit Polyclonal Anti-MTBP Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP17569PU-N | MTBP (C-term) antibody |
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 |
Rabbit | Hu | WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
