MTF1 antibody
Principal name
MTF1 antibody
Alternative names for MTF1 antibody
Transcription factor MTF-1, MRE-binding transcription factor, Metal regulatory transcription factor 1
Gene ID
4520 (MTF1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of MTF1 antibody
- Kit Component:
- - KN204861G1, MTF1 gRNA vector 1 in pCas-Guide vector
- KN204861G2, MTF1 gRNA vector 2 in pCas-Guide vector
- KN204861D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Chen,X., et al., (2004) J. Biol. Chem. 279 (6), 4515-4522
Detection limit for recombinant GST tagged MTF1 is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged MTF1 is approximately 0.03ng/ml as a capture antibody.
Detection limit for recombinant GST tagged MTF1 is approximately 0.03ng/ml as a capture antibody.
Human HepG2; WB Suggested Anti-MTF1 Antibody Titration: 5.0ug/ml. ELISA Titer: 1:2500. Positive Control: HepG2 cell lysate. MTF1 is supported by BioGPS gene expression data to be expressed in HepG2.; MTF1 antibody - C-terminal region (ARP32027_T100) in Human HepG2 cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTF1 (RC204861, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTF1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTF1 (RC204861, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTF1.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MTF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MTF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MTF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MTF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MTF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MTF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MTF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MTF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA805480)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTF1 (RC204861, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTF1.(1:2000)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MTF1 mouse monoclonal antibody. (TA805483) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTF1 (RC204861, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTF1.
WB Suggested Anti-MTF1 Antibody Titration: 5.0 ug/ml; ELISA Titer: 1:2500; Positive Control: HepG2 cell lysateMTF1 is supported by BioGPS gene expression data to be expressed in HepG2
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA805480 | MTF1 antibody |
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| MTF1 mouse monoclonal antibody, clone OTI2F3 (formerly 2F3) | |||||||
 |
Mouse | IgG1 | OTI2F3 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +8 additional images |
Highly validated antibody!
|
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| TA805437 | MTF1 antibody |
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| MTF1 mouse monoclonal antibody, clone OTI1D3 (formerly 1D3) | |||||||
 |
Mouse | IgG1 | OTI1D3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA805438 | MTF1 antibody |
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| MTF1 mouse monoclonal antibody, clone OTI1G4 (formerly 1G4) | |||||||
 |
Mouse | IgG2b | OTI1G4 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA805483 | MTF1 antibody |
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| MTF1 mouse monoclonal antibody, clone OTI7D6 (formerly 7D6) | |||||||
 |
Mouse | IgG2b | OTI7D6 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304861 | MTF1 |
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| Recombinant protein of human metal-regulatory transcription factor 1 (MTF1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401804 | MTF1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
