MTHFD2 antibody
Principal name
MTHFD2 antibody
Alternative names for MTHFD2 antibody
NMDMC, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, NADP+ dependent
Ncbi ID
9606, NP_006627, NM_006636, NP_006627.2, NP_032664.1, NM_008638
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)
Background of MTHFD2 antibody
- Kit Component:
- - KN310480G1, Mthfd2 gRNA vector 1 in pCas-Guide vector
- KN310480G2, Mthfd2 gRNA vector 2 in pCas-Guide vector
- KN310480D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Watkins D., et al. (2011) Genet. 48 (9): 590–2.
Immunoperoxidase of monoclonal antibody to MTHFD2 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3ug/ml]
Western Blot analysis of MTHFD2 expression in transfected 293T cell line by MTHFD2 monoclonal antibody (M01), clone 4G7-2G3.Lane 1: MTHFD2 transfected lysate(26.8 KDa).Lane 2: Non-transfected lysate.
MTHFD2 monoclonal antibody (M01), clone 4G7-2G3 Western Blot analysis of MTHFD2 expression in HepG2 ( Cat # L019V1 ).
Western Blot analysis of MTHFD2 expression in transfected 293T cell line (H00010797-T01) by MTHFD2 MaxPab polyclonal antibody.Lane 1: MTHFD2 transfected lysate(27.28 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MTHFD2 expression in transfected 293T cell line (H00010797-T02) by MTHFD2 MaxPab polyclonal antibody.Lane 1: MTHFD2 transfected lysate(26.80 KDa).Lane 2: Non-transfected lysate.
Human HepG2; WB Suggested Anti-MTHFD2 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate; MTHFD2 antibody - N-terminal region (ARP46038_T100) in Human HepG2 cells using Western Blot
Western Blot analysis of MTHFD2 expression in transfected 293T cell line (H00010797-T01) by MTHFD2 MaxPab polyclonal antibody.Lane 1: MTHFD2 transfected lysate(27.28 KDa).Lane 2: Non-transfected lysate.
Transfected 293T; WB Suggested Anti-MTHFD2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; MTHFD2 antibody - middle region (ARP46039_P050) in Transfected 293T cells using Western Blot
Human 293T; WB Suggested Anti-MTHFD2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate. MTHFD2 is supported by BioGPS gene expression data to be expressed in HEK293T.; MTHFD2 antibody - N-terminal region (ARP46160_P050) in Human 293T cells using Western Blot
Transfected 293T; WB Suggested Anti-MTHFD2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; MTHFD2 antibody - N-terminal region (ARP46163_P050) in Transfected 293T cells using Western Blot
Western blot analysis of MTHFD2 antibody (C-term) (Cat.# TA324931) in NCI-H460 cell line lysates (35ug/lane). MTHFD2 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human Colon carcinoma reacted with MTHFD2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
MTHFD2 Antibody (C-term) (Cat. #TA324931) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-MTHFD2 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate
WB Suggested Anti-MTHFD2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T
WB Suggested Anti-MTHFD2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 293T cell lysate.MTHFD2 is supported by BioGPS gene expression data to be expressed in HEK293T
WB Suggested Anti-MTHFD2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Transfected 293T
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324931 | MTHFD2 antibody |
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| Rabbit polyclonal MTHFD2 Antibody (C-term) | |||||||
 |
Rabbit | IgG | Purified | Hu | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302143 | MTHFD2 |
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| Recombinant protein of human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), nuclear gene encoding mitochondrial protein, tra | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401984 | MTHFD2 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |