Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » MTNR1A antibody

MTNR1A antibody

(reset)

Principal name

MTNR1A antibody

Alternative names for MTNR1A antibody

MTNR1A, Melatonin receptor type 1A, Mel1a melatonin receptor, Mel-1A-R

SwissProt ID

O02769 (Bovin), O02781 (Pig), P48039 (Human), P48040 (Sheep), P49217 (Phosu), P49218 (Rat), P49285 (Chick), P51048 (Xenla), Q61184 (Mouse)

Gene ID

4543 (MTNR1A), 17773 (Mtnr1a)

Ncbi ID

9606, NP_005949.1, NM_005958, NP_005949, NP_032665.1, NM_008639

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Fi (Fish), Hst (Hamster), Marmoset, Mky (Monkey), Op (Opossum), Prim (Primate), Xen (Xenopus), Ze (Zebrafish), Tr (Turkey), L (Lizard), Bear, Sh (Sheep)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)

Background of MTNR1A antibody

Melatonin (N-acetyl-5-methoxytryptamine) is a product of tryptophan metabolism. It is synthesized in the pineal gland and is secreted to control the circadian rhythm. Melatonin is also synthesized in the gastrointestil tract, reti, skin and other tissues where it acts in a autocrine or paracrine manner. The role of melatonin in these tissues is independent of its role in the circadian rhythm, where it plays a role in energy metabolism, physiological growth, differentiation and responsiveness in stress stimuli. The pleiotropic effects of melatonin have given rise to various therapeutic possibilities for this molecule. For example; anti-stress, sexual dysfunction, obesity, gallbladder stones. To date, the only therapeutic uses for melatonin remain to treat sleep disorders, depression, migraine and headaches. Melatonin exerts its effects through two G-protein coupled receptors (GPCRs); melatonin receptor type 1 and melatonin receptor type 2 (MT1 and MT2). Like all GPCRs, they have seven transmembrane domains and extracellular N-termil and cytoplasmic C-termil tails. The binding of melatonin to either receptor activates Gi, thereby activating PLC, thus increasing intracellular Ca2+ levels. Both receptors structurally bind melatonin in the same manner, although MT2 displays a much higher affinity for the hormone. Just like melatonin levels are detected in many tissues, the expression patterns of the two receptors are also quite broad. For example, MT1 is detected in the brain, reti and kidneys and MT2 is expressed in brain and in the reti. MT1 is involved in sleep regulation and might also have effects on peripheral vasoconstriction. MT2 may play an important physiological role in the reti and might regulate body temperature.

General readings

Adi,N. et.al., Med. Sci. Monit. 16 (2): BR61-BR67 (2010)
Hill,S.M. et.al., Integr Cancer Ther 8 (4): 337-346 (2009)
Lai,L. et.al., Breast Cancer Res. Treat. 118 (2): 293-305 (2009)

PAB13671 - MTNR1A
MTNR1A polyclonal antibody ( Cat # PAB13671 ) ( 0.3µg/ml ) staining of KELLY cell lysate ( 35µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
G398-1 - MTNR1A
Immunofluorescence analysis of HepG2 cells, using MTR1A Antibody. The picture on the right is treated with the synthesized peptide.
ARP61495_P050 - MTNR1A
Human Jurkat; WB Suggested Anti-MTNR1A Antibody. . Titration: 1.0 ug/ml. . Positive Control: Jurkat Whole Cell; MTNR1A antibody - C-terminal region (ARP61495_P050) in Human Jurkat cells using Western Blot
ARP61495_P050 - MTNR1A
Human 293T; Host: Rabbit. Target Name: HIRIP3 . Sample Tissue: 293T . Antibody Dilution: 1.0ug/ml.; MTNR1A antibody - C-terminal region (ARP61495_P050) in Human 293T cells using Western Blot
AP52769PU-N - MTNR1A
Western blot analysis of MTNR1A (arrow) in CEM cell line lysates (35ug/lane) using MTNR1A antibody Cat.-No. AP52769PU-N.
AP52769PU-N - MTNR1A
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram) using MTNR1A antibody Cat.-No. AP52769PU-N, followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
TP310385 - MTNR1A
TA305660 - MTNR1A
TA305660 (0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA308321 - MTNR1A
Melatonin Receptor 1A antibody detects Melatonin Receptor 1A protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Melatonin Receptor 1A antibody (GTX100003) diluted at 1:1000.
TA312291 - MTNR1A
Immunofluorescence analysis of HepG2 cells, using MTR1A antibody.The picture on the right is treated with the synthesized peptide.
TA321735 - MTNR1A
Predicted band size: 39 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/650 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA321735 - MTNR1A
Predicted cell location: Cytoplasm, cell membrane. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using MTNR1A antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA321736 - MTNR1A
Predicted band size: 39 kDa . Positive control: NIH/3T3 cell lysate. Recommended dilution: 1/1000-3000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/1700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA321736 - MTNR1A
Predicted cell location: Cytoplasm, cell membrane. Positive control: Human breast cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using MTNR1A antibody at dilution 1/100, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA328874 - MTNR1A
Western blot analysis of mouse brain (lanes 1 and 4) rat brain (lanes 2 and 5) and rat cerebellum (lanes 3 and 6) lysates: 1-3. Anti-Melatonin Receptor Type 1 antibody, (1:200). 4-6. Anti-Melatonin Receptor Type 1 antibody, preincubated with the control peptide antigen.
TA328874 - MTNR1A
Expression of Melatonin receptor type 1 in rat SCN. Immunohistochemical staining of rat supra-chiasmatic nucleus (SCN) with Anti-Melatonin Receptor Type 1 antibody . A. MT1 staining is restricted to the SCN (red). B.DAPI is used as the counterstain (blue). C. Merge of A and B.
TA341365 - MTNR1A
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
TA341365 - MTNR1A
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
TA341365 - MTNR1A
Anti-MTNR1A / Melatonin Receptor 1a antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA341366 - MTNR1A
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
TA341366 - MTNR1A
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MTNR1A antibody
  Goat Anti-MTNR1A Antibody  
TA305660 (0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

MTNR1A antibody
  Rabbit polyclonal antibody to MEL-1A-R (melatonin receptor 1A)  
Melatonin Receptor 1A antibody detects Melatonin Receptor 1A protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Melatonin Receptor 1A antibody (GTX100003) diluted at 1:1000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €240.00
  OriGene Technologies, Inc.

MTNR1A antibody
  Rabbit polyclonal anti-MTR1A antibody  
Immunofluorescence analysis of HepG2 cells, using MTR1A antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF
0.1 ml / €335.00
  OriGene Technologies, Inc.

MTNR1A antibody
  Anti-MTNR1A Rabbit Polyclonal Antibody  
Predicted band size: 39 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/650 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

MTNR1A antibody
  Anti-MTNR1A Rabbit Polyclonal Antibody  
Predicted band size: 39 kDa . Positive control: NIH/3T3 cell lysate. Recommended dilution: 1/1000-3000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/1700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

MTNR1A antibody
  Rabbit Polyclonal Anti-Melatonin Receptor type 1  
Western blot analysis of mouse brain (lanes 1 and 4) rat brain (lanes 2 and 5) and rat cerebellum (lanes 3 and 6) lysates: 1-3. Anti-Melatonin Receptor Type 1 antibody, (1:200). 4-6. Anti-Melatonin Receptor Type 1 antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €585.00
  OriGene Technologies, Inc.
+1 additional image

MTNR1A (2nd. Cytoplasm. Dom.) antibody
  Rabbit Polyclonal Anti-MTNR1A Antibody (Cytoplasmic Domain)  
MTNR1A Rabbit Aff - Purified Bear, Bov, Can, Fi, Hst, Hu, L, Marmoset, Mky, Ms, Op, Prim, Rt, Tr, Xen, Ze P
50 µg / €425.00
  OriGene Technologies, Inc.

MTNR1A (1st Cytopl. Dom.) antibody
  Rabbit Polyclonal Anti-MTNR1A Antibody (Cytoplasmic Domain)  
MTNR1A Rabbit Aff - Purified Bov, Can, Fi, Hu, L, Mky, Ms, Prim, Rt, Sh, Xen P
50 µg / €425.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MTNR1A
  Recombinant protein of human melatonin receptor 1A (MTNR1A)  
MTNR1A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

MTNR1A overexpression lysate
MTNR1A overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

10 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (8)
  3. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (8)
Target
  1. MTNR1A (9)
Property
  1. 1st Cytopl. Dom. (1)
  2. 2nd. Cytoplasm. Dom. (1)
Reactivity
  1. Human (Hu) (7)
  2. Mouse (Ms) (6)
  3. Rat (Rt) (5)
  4. Bovine (Bov) (2)
  5. Canine (Can) (2)
  6. Fish (Fi) (2)
  7. Lizard (L) (2)
  8. Monkey (Mky) (2)
  9. Primate (Prim) (2)
  10. Xenopus (Xen) (2)
  11. Bear (1)
  12. Hamster (Hst) (1)
  13. Marmoset (1)
  14. Opossum (Op) (1)
  15. Sheep (Sh) (1)
  16. Turkey (Tr) (1)
  17. Zebrafish (Ze) (1)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (4)
  3. Enzyme Immunoassay (E) (3)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Aff - Purified (4)
  2. Purified (4)
Host
  1. Rabbit (7)
  2. Goat (1)
Images
  1. 1 image (7)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. IHC (3)
  3. Immunofluorescence (IF) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn