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MYL12A antibody

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Principal name

MYL12A antibody

Alternative names for MYL12A antibody

MLCB, MRLC3, RLC, MLC-2B, Myosin RLC, Myosin regulatory light chain 12A, Myosin regulatory light chain 2 nonsarcomeric, Myosin regulatory light chain MRLC3

SwissProt ID

P19105 (Human)

Gene ID

10627 (MYL12A)

Ncbi ID

NP_006462, NM_006471, NP_006462.1

Available reactivities

Av (Birds), Hu (Human), Mam (Mammalia), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of MYL12A antibody

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human and mouse have almost identical sequences. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.

General readings

Yu L.-R., et al. (2007) J. Proteome Res. 6:4150-4162.

AR39103PU-L - MYL12A
AR39103PU-L - MYL12A
MYL12A, 1-171aa, Human, His-tagged
TP307294 - MYL12A
TA319238 - MYL12A
Phospho specific antibody to Monophosphorylated Regulatory Light Chain of Smooth and Non-muscle Myosin at pS19/pS20 was used at a 1:5000 dilution to detect myosin light chain by WB. Either 13 or 20 µl of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE.  Samples were either mock-treated or CLA-treated, as indicated.  After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.
TA319238 - MYL12A
Phosphospecific antibody to phosphorylated regulatory light chain of smooth and non-muscle Myosin at pS19/pS20 was used at a 1:1000 dilution to detect myosin light chain by WB on 3T3 cell lysates.
TA319238 - MYL12A
Anti-Monophosphorylated RLC Smooth and Non-Muscle Myosin pS19/20 antibody was used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MYL12A pSer19/20 antibody
  Rabbit polyclonal Myosin phospho S19/phospho S20 antibody  
Phospho specific antibody to Monophosphorylated Regulatory Light Chain of Smooth and Non-muscle Myosin at pS19/pS20 was used at a 1:5000 dilution to detect myosin light chain by WB. Either 13 or 20 µl of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE.  Samples were either mock-treated or CLA-treated, as indicated.  After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system. Rabbit Aff - Purified Av, Hu, Mam E, IP, P, WB
0.1 mg / €555.00
  OriGene Technologies, Inc.
+2 additional images

Published recently!

MYL12A antibody
  Phospho-MYL12A-S18 Rabbit Polyclonal Antibody  
MYL12A Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MYL12A
  Recombinant protein of human myosin, light chain 12A, regulatory, non-sarcomeric (MYL12A)  
MYL12A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

MYL12A overexpression lysate
MYL12A overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

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Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (2)
  3. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (2)
Target
  1. MYL12A (3)
Property
  1. pSer19/20 (1)
Reactivity
  1. Human (Hu) (2)
  2. Birds (Av) (1)
  3. Mammalia (Mam) (1)
  4. Mouse (Ms) (1)
  5. Rat (Rt) (1)
Applications
  1. Enzyme Immunoassay (E) (1)
  2. Immunoprecipitation (IP) (1)
  3. Paraffin Sections (P) (1)
  4. Western blot / Immunoblot (WB) (1)
Presentation
  1. Aff - Purified (1)
  2. Purified (1)
Host
  1. Rabbit (2)
Images
  1. 1 image (2)
  2. 3+ images (1)
Images: Applications
  1. IHC (1)
  2. Western blot / Immunoblot (WB) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (4)
Service
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