MYL6 antibody
Principal name
MYL6 antibody
Alternative names for MYL6 antibody
Myosin light polypeptide 6, LC17, MLC-3, Myosin light chain 3, Myosin light chain alkali 3, 17 kDa myosin light chain, Smooth muscle and nonmuscle myosin light chain alkali 6
Ncbi ID
9606, NP_524147, NP_066299, NP_066299.2, NM_079423, NM_021019, NM_010860, NM_001100983, BC081470
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)
Background of MYL6 antibody
Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, mouse and rat have almost identical sequences.
General readings
Fu,Z.Y., (2006) Acta Biochim. Biophys. Sin. (Shanghai) 38 (9), 625-632
Detection limit for recombinant GST tagged MYL6 is approximately 0.3ng/ml as a capture antibody.
MYL6 monoclonal antibody (M03), clone 1D6. Western Blot analysis of MYL6 expression in Raw 264.7 ( Cat # L024V1 ).
Detection limit for recombinant GST tagged MYL6 is approximately 1ng/ml as a capture antibody.
Immunohistochemical staining with MYL6 (phospho S19/20) polyclonal antibody ( Cat # PAB9961 ) was used at 2.5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus.Tissue was formalin-fixed and paraffin embedded.The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
MYL6 (phospho S19/20) polyclonal antibody ( Cat # PAB9961 ) was used at a 1 : 1000 dilution to detect myosin light chain by Western blot on NIH/3T3 cell lysates.A standard urea/glycerol gel without SDS was used to separate phospho forms of regulatory light chain according to mass to charge ratios.In Panel A, reactivity of MYL6 (phospho S19/20) polyclonal antibody ( Cat # PAB9961 ) is shown.In Panel B, reactivity of commercially available pan reactive antibody that detects both unphosphorylated and phosphorylated forms of regulatory light chain is shown.The phosphospecific antibody detects both monophosphorylated ( pSer20 Mono-P-RLC ) and diphosphorylated ( pThr19-pSer20 Di-P-RLC ) regulatory light chain.Personal communication. J. Stull. UT Southwestern Medical Center.
Human Prostate; MYL6 antibody - middle region (ARP57712_P050) in Human Prostate cells using Immunohistochemistry
Human Skeletal Muscle; Immunohistochemistry with Human Skeletal Muscle lysate tissue at an antibody concentration of 5.0ug/ml using anti-MYL6 antibody (ARP57712_P050); MYL6 antibody - middle region (ARP57712_P050) in Human Skeletal Muscle cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-MYL6 Antibody Titration: 1 ug/ml. Positive Control: HepG2 cell lysate. MYL6 is supported by BioGPS gene expression data to be expressed in HepG2.; MYL6 antibody - middle region (ARP57712_P050) in Human HepG2 cells using Western Blot
Human Skeletal Muscle; MYL6 antibody - N-terminal region (ARP57711_P050) in Human Skeletal Muscle cells using Immunohistochemistry
Human Prostate; MYL6 antibody - N-terminal region (ARP57711_P050) in Human Prostate cells using Immunohistochemistry
Human 721_B; Host:Rabbit. Target Name:MYL6. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. MYL6 is supported by BioGPS gene expression data to be expressed in 721_B.; MYL6 antibody - N-terminal region (ARP57711_P050) in Human 721_B cells using Western Blot
Human HepG2; WB Suggested Anti-MYL6 Antibody Titration: 1 ug/ml. Positive Control: HepG2 cell lysate. MYL6 is supported by BioGPS gene expression data to be expressed in HepG2.; MYL6 antibody - N-terminal region (ARP57711_P050) in Human HepG2 cells using Western Blot
Recombinant human MYL6, 1-151 aa, His-tagged
Western blot analysis of MYL6 expression in HeLa (A); K562 (B); SP2/0 (C); H9C2 (D) whole cell lysates.
Western blot analysis of extracts from K562 cells, using MYL6 antibody.The lane on the right is treated with the synthesized peptide.
WB using anti-RLC of Smooth and Non-muscle Myosin antibody to detect vascular myosin (rat aorta, lane 1) but not cardiac myosin (mouse heart, lane2).The primary antibody was diluted in blocking buffer to 1:600 for 2 h at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
WB Suggested Anti-MYL6 Antibody Titration: 1 ug/ml; Positive Control: HepG2 cell lysateMYL6 is supported by BioGPS gene expression data to be expressed in HepG2
Immunohistochemistry with Human Skeletal Muscle lysate tissue at an antibody concentration of 5.0 ug/ml using anti-MYL6 antibody
Human Prostate
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Mouse stomach tissue, Primary antibody: (MYL6 Antibody) at dilution 1/262.5, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (MYL6 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (MYL6 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314227 | MYL6 antibody |
||||||
| Rabbit polyclonal anti-MYL6 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA319521 | MYL6 (12-27) antibody |
||||||
| Rabbit polyclonal anti-Myosin antibody | |||||||
![WB using anti-RLC of Smooth and Non-muscle Myosin antibody to detect vascular myosin (rat aorta, lane 1) but not cardiac myosin (mouse heart, lane2).The primary antibody was diluted in blocking buffer to 1:600 for 2 h at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/600-401-938-1-W.jpg) |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€515.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA349409 | MYL6 antibody |
||||||
| Rabbit Polyclonal Anti-MYL6 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300708 | MYL6 (transcript variant 1) |
||||
| Recombinant protein of human myosin, light chain 6, alkali, smooth muscle and non-muscle (MYL6), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP324925 | MYL6 (transcript variant 2) |
||||
| Recombinant protein of human myosin, light chain 6, alkali, smooth muscle and non-muscle (MYL6), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY409203 | MYL6 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY412144 | MYL6 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201072L2V | Lenti ORF particles, Myl6 (GFP-tagged) - Mouse myosin, light polypeptide 6, alkali, smooth muscle and non-muscle (cDNA clone MGC:102200 IMAGE:6824167), complete, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€840.00
|
|
| OriGene Technologies, Inc. | ||
| MR201079L2V | Lenti ORF particles, Myl6 (GFP-tagged) - Mouse myosin, light polypeptide 6, alkali, smooth muscle and non-muscle (Myl6), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201079L1V | Lenti ORF particles, Myl6 (Myc-DDK-tagged) - Mouse myosin, light polypeptide 6, alkali, smooth muscle and non-muscle (Myl6), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR203234L2V | Lenti ORF particles, Myl6l (GFP-tagged ORF) - Rat myosin, light polypeptide 6, alkali, smooth muscle and non-muscle-like (Myl6l), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR203234L1V | Lenti ORF particles, Myl6l (Myc-DDK-tagged ORF) - Rat myosin, light polypeptide 6, alkali, smooth muscle and non-muscle-like (Myl6l), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
