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MYLK antibody

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Principal name

MYLK antibody

Alternative names for MYLK antibody

Myosin light chain kinase, smooth muscle, MLCK, Telokin, Kinase-related protein

SwissProt ID

O02827 (Sheep), P11799 (Chick), Q15746 (Human), Q28824 (Bovin), Q6PDN3 (Mouse)

Gene ID

4638 (MYLK)

Ncbi ID

9606, 5874, NP_444253, ENSP00000355024, NP_444253.2, NP_444253.3, NM_053031, NP_444259, NM_053025

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Mky (Monkey), Rb (Rabbit), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of MYLK antibody

MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

PAB3181 - MYLK
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MYLK polyclonal antibody ( Cat # PAB3181 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
PAB3181 - MYLK
Western blot analysis of MYLK polyclonal antibody ( Cat # PAB3181 ) in A-375 cell lysate. MYLK ( Arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
PAB3182 - MYLK
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MYLK polyclonal antibody ( Cat # PAB3182 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
PAB3182 - MYLK
Western blot analysis of MYLK polyclonal antibody ( Cat # PAB3182 ) in mouse brain tissue lysate. MYLK ( Arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
H00004638-B01 - MYLK
MYLK MaxPab polyclonal antibody. Western Blot analysis of MYLK expression in human kidney.
H00004638-B01 - MYLK
Western Blot analysis of MYLK expression in transfected 293T cell line (H00004638-T01) by MYLK MaxPab polyclonal antibody.Lane 1: MYLK transfected lysate(109.12 KDa).Lane 2: Non-transfected lysate.
H00004638-D01P - MYLK
Proximity Ligation Analysis (PLA) of protein-protein interactions between MYLK and CTTN. HeLa cells were stained with anti-MYLK rabbit purified polyclonal 1:1200 and anti-CTTN mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
H00004638-D01P - MYLK
MYLK MaxPab rabbit polyclonal antibody. Western Blot analysis of MYLK expression in human stomach.
H00004638-D01P - MYLK
Western Blot analysis of MYLK expression in transfected 293T cell line (H00004638-T01) by MYLK MaxPab polyclonal antibody.Lane 1: MYLK transfected lysate(110.20 KDa).Lane 2: Non-transfected lysate.
AP14867PU-N - MYLK
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP14867PU-N - MYLK
Western blot analysis of anti-MLCK-long Pab in mouse brain tissue lysate. MLCK-long (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
CPA1783-100ul - MYLK
Western blot analysis of MLCK1 expression in HeLa (A); SP2/0 (B); H9C2 (C) whole cell lysates.
TP318513 - MYLK
TA311559 - MYLK
(0.03ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA312615 - MYLK
Western blot analysis of extracts from HepG2 cells, using MYLK antibody.The lane on the right is treated with the synthesized peptide.
TA347970 - MYLK
Western blot analysis of MYLK expression in HepG2 cells

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MYLK antibody
  Goat Anti-MYLK Antibody  
(0.03ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

MYLK antibody
  Rabbit polyclonal anti-MYLK antibody  
Western blot analysis of extracts from HepG2 cells, using MYLK antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

MYLK antibody
  Rabbit Polyclonal Anti-MYLK Antibody  
Western blot analysis of MYLK expression in HepG2 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

MYLK (N-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MYLK (transcript variant 7)
  Recombinant protein of human myosin light chain kinase (MYLK), transcript variant 7  
MYLK Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

MYLK overexpression lysate
MYLK overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, MYLK (Myc-DDK tagged) - Human myosin light chain kinase (MYLK), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, MYLK (Myc-DDK tagged) - Human myosin light chain kinase (MYLK), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €2,420.00
  OriGene Technologies, Inc.
 

7 Item(s)

per page

Available Filters

Product Type
  1. Human Lenti ORF Particle (1)
  2. Lysate (1)
  3. Primary Antibody (4)
  4. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (4)
Target
  1. MYLK (5)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (4)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Enzyme Immunoassay (E) (2)
  3. Paraffin Sections (P) (1)
Presentation
  1. Purified (3)
  2. Aff - Purified (1)
Host
  1. Rabbit (3)
  2. Goat (1)
Images
  1. 1 image (5)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (7)
Service
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