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Myostatin antibody

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Principal name

Myostatin antibody

Alternative names for Myostatin antibody

MSTN, GDF8, GDF-8, Growth differentiation factor 8

SwissProt ID

O08689 (Mouse), O14793 (Human), O18830 (Sheep), O18831 (Pig), O18836 (Bovin), O35312 (Rat)

Gene ID

2660 (MSTN), 17700 (Mstn)

Ncbi ID

NP_005250.1, NP_005250, 9606, NP_034964.1, O14793, NM_010834, NM_005259

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine), Rb (Rabbit), Sh (Sheep), Ze (Zebrafish)

Available hosts

Chicken, Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Functional assay (FN)

Background of Myostatin antibody

Myostatin (GDF8) is expressed uniquely in human skeletal muscle as a 12 kDa mature glycoprotein consisting of 113 amino acid residues and secreted into plasma. Myostatin is a member of the transforming growth factor ß superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology.
In serum, myostatin circulates as part of a latent complex containing myostatin propeptide and/or follistatin-related gene. The myostatin propeptide is known to bind and inhibit myostatin in vitro. This interaction is relevant in vivo, with a majority (>70%) of myostatin in serum bound to its propeptide. The myostatin propeptide is negative regulator of myostatin in vivo.

General readings

1. McPherron AC, Lee SJ.
2. Jiang MS, Liang LF, Wang S, Ratovitski T, Holmstrom J, Barker C, Stotish R. Characterization and identification of the inhibitory domain of GDF-8 propeptide.
3. McPherron A.C. and Lee S.J. Double muscling in cattle due to mutations in the myostatin gene.
4. Thies RS, Chen T, Davies MV, Tomkinson KN, Pearson AA, Shakey QA, Wolfman NM. GDF-8 propeptide binds to GDF-8 and antagonizes biological activity by inhibiting GDF-8 receptor
binding.
5. Taylor WE, Bhasin S, Artaza J, Byhower F, Azam M, Willard DH Jr, Kull FC Jr, Gonzalez-Cadavid N. Myostatin inhibits cell proliferation and protein synthesis in C2C12 muscle cells.
6. Artaza JN, Bhasin S, Magee TR, Reisz-Porszasz S, Shen R, Groome NP, Fareez MM, Gonzalez-Cadavid NF. Myostatin inhibits myogenesis and promotes adipogenesis in C3H 10T (1/2) mesenchymal multipotent cells. Endocrinology.
7. Sharma M. et al. Myostatin, a transforming growth factor beta superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct.
8. Sharma M, Kambadur R, Matthews KG, Somers WG, Devlin GP, Conaglen JV, Fowke PJ, Bass JJ. Myostatin, a transforming growth factor-beta superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct.
9. Gonzalez-Cadavid NF, Taylor WE, Yarasheski K, Sinha-Hikim I, Ma K, Ezzat S, Shen R, Lalani R, Asa S, Mamita M, Nair G, Arver S, Bhasin S. Organization of the human myostatin gene and expression in healthy men and HIV-infected men with muscle wasting.
10. Gonzalez Cadavid N.F., Taylor W.E. et al. Organization of the human myostatin gene and expression in heathy men and HIV infected men with muscle wasting.
11. Lee SJ, McPherron AC. Regulation of myostatin activity and muscle growth.
12. Hill JJ, Davies MV, Pearson AA, Wang JH, Hewick RM, Wolfman NM, Qiu Y. The myostatin propeptide and the follistatin-related gene are inhibitory binding proteins of myostatin in normal serum.

PA512X - Myostatin
12% SDS-PAGE separation of Human Myostatin: 1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa; 2. reduced and heated sample, 10 µg/lane; 3. non-reduced and non-heated sample, 10 µg/lane.
H00002660-M07 - Myostatin
Western Blot analysis of GDF8 expression in transfected 293T cell line by GDF8 monoclonal antibody (M07), clone 3E7.Lane 1: GDF8 transfected lysate(42.8 KDa).Lane 2: Non-transfected lysate.
H00002660-M07 - Myostatin
GDF8 monoclonal antibody (M07), clone 3E7 Western Blot analysis of GDF8 expression in LNCaP ( Cat # L004V1 ).
H00002660-M06 - Myostatin
Western Blot analysis of GDF8 expression in transfected 293T cell line by GDF8 monoclonal antibody (M06), clone 2D8.Lane 1: GDF8 transfected lysate(42.8 KDa).Lane 2: Non-transfected lysate.
AP02127PU-S - Myostatin
Immunofluorescence of Myostatin in Paraffin Section of Human finger pad. The section was incubated with Myostatin antibody Cat.-No AP02127PU (1/100) followed by an appropriate secondary antibody coupled to Cy3. Original magnification: x40
PAB3887 - Myostatin
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MSTN polyclonal antibody ( Cat # PAB3887 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PAB3887 - Myostatin
Western blot analysis of MSTN polyclonal antibody ( Cat # PAB3887 ) in mouse liver tissue lysate. MSTN ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
AP11945PU-N - Myostatin
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GDF8 antibody (N-term )(AP11945PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP11945PU-N - Myostatin
Western blot analysis of anti-GDF8 N-term (AP11945PU-N) in mouse liver tissue lysate. GDF8 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence
AM20481SU-N - Myostatin
Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20481SU-N - Myostatin
Western blot analysis using anti-Myostatin monoclonal antibody against truncated Myostatin -His recombinant protein (Lane 1).
AM06308SU-N - Myostatin
Western blot analysis using Myostatin mouse mAb against truncated Myostatin-His recombinant protein (1).
AR51737PU-N - Myostatin
TA807788 - Myostatin
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSTN (RC210368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSTN.(1:2000)
TA807789 - Myostatin
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSTN (RC210368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSTN.(1:2000)
TA807790 - Myostatin
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSTN (RC210368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSTN.(1:2000)
TA807791 - Myostatin
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSTN (RC210368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSTN.(1:2000)
TA807820 - Myostatin
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSTN (RC210368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSTN.(1:500)
TP310368 - Myostatin
TP723323 - Myostatin
TA317041 - Myostatin
Anti-MSTN / Myostatin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
TA321697 - Myostatin
Predicted cell location: Cytoplasm. Positive control: Human breast and lung cancer tissue. Recommended dilution: 1/5-20 The image on the left is immunohistochemistry of paraffin-embedded Human breast and lung cancer tissue using MSTN antibody at dilution 1/8, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA321697 - Myostatin
Predicted cell location: Cytoplasm. Positive control: Human breast and lung cancer tissue. Recommended dilution: 1/5-20 The image on the left is immunohistochemistry of paraffin-embedded Human breast and lung cancer tissue using MSTN antibody at dilution 1/8, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA343358 - Myostatin
Host: Rabbit; Target Name: Mstn; Sample Tissue: Mouse Brain lysates; Antibody Dilution: 1.0 ug/ml

Items 1 to 20 of 34 total

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Myostatin antibody
  Anti-MSTN Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human breast and lung cancer tissue. Recommended dilution: 1/5-20 The image on the left is immunohistochemistry of paraffin-embedded Human breast and lung cancer tissue using MSTN antibody at dilution 1/8, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

Myostatin antibody
  MSTN mouse monoclonal antibody, clone OTI7H5 (formerly 7H5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSTN (RC210368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSTN.(1:2000) Mouse IgG1 OTI7H5 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

Myostatin antibody
  MSTN mouse monoclonal antibody, clone OTI4F9 (formerly 4F9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSTN (RC210368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSTN.(1:2000) Mouse IgG2b OTI4F9 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

Myostatin antibody
  MSTN mouse monoclonal antibody, clone OTI6A7 (formerly 6A7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSTN (RC210368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSTN.(1:2000) Mouse IgG1 OTI6A7 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

Myostatin antibody
  MSTN mouse monoclonal antibody, clone OTI3A7 (formerly 3A7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSTN (RC210368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSTN.(1:2000) Mouse IgG2b OTI3A7 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

Myostatin antibody
  MSTN mouse monoclonal antibody, clone OTI2D10 (formerly 2D10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSTN (RC210368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSTN.(1:500) Mouse IgG1 OTI2D10 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

Myostatin antibody
Myostatin Mouse IgG2b 7F Purified Hu E
0.1 mg / €450.00
  OriGene Technologies GmbH

Myostatin antibody
Myostatin Mouse IgG2b 7F Purified Hu E
10 µg / €140.00
  OriGene Technologies GmbH

Myostatin antibody
Myostatin Mouse IgG2b 4D Purified Hu E
0.1 mg / €450.00
  OriGene Technologies GmbH

Myostatin antibody
Myostatin Mouse IgG2b 4D Purified Hu E
10 µg / €140.00
  OriGene Technologies GmbH

Myostatin antibody
Western blot analysis using Myostatin mouse mAb against truncated Myostatin-His recombinant protein (1). Mouse IgG1 6H12 Ascites Hu WB
0.1 ml / €340.00
  OriGene Technologies GmbH

Myostatin (24-266) antibody
Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 6H12 Ascites Hu E, P, WB
50 µl / €425.00
  OriGene Technologies GmbH
+1 additional image

Myostatin (53-66) antibody
Myostatin Rabbit Serum Hu E
0.1 ml / €450.00
  OriGene Technologies GmbH

Myostatin (53-66) antibody
Myostatin Rabbit Serum Hu E
20 µl / €180.00
  OriGene Technologies GmbH

Myostatin (79-92) antibody
Myostatin Rabbit Serum Hu E
0.1 ml / €450.00
  OriGene Technologies GmbH

Myostatin (79-92) antibody
Myostatin Rabbit Serum Hu E
20 µl / €180.00
  OriGene Technologies GmbH

Myostatin (83-98) antibody
Myostatin Rabbit Serum Hu E
0.1 ml / €450.00
  OriGene Technologies GmbH

Myostatin (83-98) antibody
Myostatin Rabbit Serum Hu E
20 µl / €180.00
  OriGene Technologies GmbH

Myostatin antibody
Immunofluorescence of Myostatin in Paraffin Section of Human finger pad. The section was incubated with Myostatin antibody Cat.-No AP02127PU (1/100) followed by an appropriate secondary antibody coupled to Cy3. Original magnification: x40 Chicken Purified Hu E, P
0.1 mg / €410.00
  OriGene Technologies GmbH

Myostatin antibody
Immunofluorescence of Myostatin in Paraffin Section of Human finger pad. The section was incubated with Myostatin antibody Cat.-No AP02127PU (1/100) followed by an appropriate secondary antibody coupled to Cy3. Original magnification: x40 Chicken Purified Hu E, P
10 µg / €150.00
  OriGene Technologies GmbH

Items 1 to 20 of 34 total

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34 products - FILTER YOUR RESULTS!

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  1. Mouse Lenti ORF Particle (1)
  2. Primary Antibody (29)
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  2. Monoclonal (11)
Target
  1. Myostatin (33)
Property
  1. 24-266 (1)
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Reactivity
  1. Human (Hu) (28)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (1)
Applications
  1. Enzyme Immunoassay (E) (22)
  2. Western blot / Immunoblot (WB) (9)
  3. Paraffin Sections (P) (5)
  4. Frozen sections (C) (1)
  5. Functional assay (FN) (1)
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Images
  1. 1 image (15)
Images: Applications
  1. Western blot / Immunoblot (WB) (8)
  2. Paraffin Sections (P) (4)
  3. IHC (1)
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