NAD-ME antibody
Principal name
NAD-ME antibody
Alternative names for NAD-ME antibody
ME2, NAD-dependent malic enzyme, mitochondrial
Ncbi ID
NP_002387, NP_002387.1, NM_002396, NP_663469.1, NM_145494, NM_001168335
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of NAD-ME antibody
- Kit Component:
- - KN309913G1, Me2 gRNA vector 1 in pCas-Guide vector
- KN309913G2, Me2 gRNA vector 2 in pCas-Guide vector
- KN309913D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
MacDonald, M.J., et al. Arch. Biochem. Biophys. 488(2):100-104(2009)
French, D., et al. Blood 113(19):4512-4520(2009)
Escamilla, M. Pharmacogenomics 8(7):691-695(2007)
Chou, W.Y., et al. Biochem. Biophys. Res. Commun. 357(1):133-138(2007)
Lenzen, K.P., et al. Epilepsia 46(10):1637-1641(2005)
Hum. Adult Placenta; Host:Rabbit. Target Name:ME2. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; ME2 antibody - N-terminal region (ARP50638_P050) in Hum. Adult Placenta cells using Western Blot
Hum. Fetal Heart; Host:Rabbit. Target Name:ME2. Sample Tissue:Human Fetal Heart. Antibody Dilution: 1.0ug/ml.; ME2 antibody - N-terminal region (ARP50638_P050) in Hum. Fetal Heart cells using Western Blot
Hum. Fetal Lung; Host:Rabbit. Target Name:ME2. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; ME2 antibody - N-terminal region (ARP50638_P050) in Hum. Fetal Lung cells using Western Blot
Human Jurkat; WB Suggested Anti-ME2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Jurkat cell lysate. ME2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; ME2 antibody - N-terminal region (ARP50638_P050) in Human Jurkat cells using Western Blot
Immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue reacted with NAD-ME Antibody (C-term) Cat.-No AP52647PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.
Western blot analysis of NAD-ME Antibody (C-term) Cat.-No AP52647PU-N in mouse NIH-3T3,Neuro-2a cell line lysates (35ug/lane).
Host: Rabbit; Target Name: ME2; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: ME2; Sample Tissue: Human Fetal Lung; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: ME2; Sample Tissue: Human Fetal Heart; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-ME2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Jurkat cell lysate ME2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
Western blot analysis of ME2 in human spleen tissue lysate with ME2 antibody at (A) 1 and (B) 2ug/mL.
Immunohistochemistry of ME2 in human spleen tissue with ME2 antibody at 5 ug/mL.
Immunofluorescence of ME2 in human spleen tissue with ME2 antibody at 20 ug/mL.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA349028 | NAD-ME antibody |
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| Rabbit Polyclonal Anti-ME2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | ICC/IF, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| AP52647PU-N | NAD-ME (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP319389 | NAD-ME |
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| Recombinant protein of human malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), nuclear gene encoding mitochondrial protein | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419348 | ME2 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209185L2V | Lenti ORF particles, Me2 (GFP-tagged) - Mouse malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,320.00
|
|
| OriGene Technologies, Inc. | ||
| MR209185L1V | Lenti ORF particles, Me2 (Myc-DDK-tagged) - Mouse malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,180.00
|
|
| OriGene Technologies, Inc. | ||