NAGK antibody
Principal name
NAGK antibody
Alternative names for NAGK antibody
N-acetyl-D-glucosamine kinase
Ncbi ID
9606, NP_060037, NP_060037.3, NP_060037.2, NP_062415.1, NM_001164187, NM_019542, NM_017567
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of NAGK antibody
- Kit Component:
- - KN310707G1, Nagk gRNA vector 1 in pCas-Guide vector
- KN310707G2, Nagk gRNA vector 2 in pCas-Guide vector
- KN310707D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Weihofen WA, Berger M, Chen H, et al. (2007). J. Mol. Biol. 364 (3): 388–99.
Weidanz JA, Campbell P, Moore D, et al. (1997). Br. J. Haematol. 95 (4): 645–53.
Hinderlich S., et al. (Jul 2000). Eur J Biochem 267 (11): 3301–8.
NAGK polyclonal antibody (A01), Lot # 060102JC01. Western Blot analysis of NAGK expression in human ovary cancer.
The NAGK polyclonal antibody ( Cat # PAB4634 ) is used in Western blot to detect NAGK in mouse thymus tissue lysate .
The NAGK polyclonal antibody ( Cat # PAB4634 ) is used in Western blot to detect NAGK in Y-79 cell lysate .
The anti-NAGK Pab is used in Western blot to detect NAGK in mouse thymus tissue lysate (Lane 1) and Y79 cell lysate (Lane 2).
Western blot analysis of NAGK (arrow) in 293 cell line lysates (35ug/lane) using NAGK antibody Cat.-No. AP13659PU-N (C-term).
Western blot analysis of NAGK (arrow) in mouse stomach tissue lysates (35ug/lane) using NAGK antibody Cat.-No. AP13659PU-N (C-term).
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NAGK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Flow cytometry analysis of NAGK in HeLa cell line, staining at 2-5ug for 1x106 cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
Immunofluorescence: ICC/IF analysis of NAGK in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NAGK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM50019PU-N | NAGK antibody |
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Mouse | IgG1 | AT4D12 | Purified | Hu | E, F, ICC/IF, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
 +2 additional images |
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| AM50019PU-S | NAGK antibody |
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|
Mouse | IgG1 | AT4D12 | Purified | Hu | E, F, ICC/IF, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP13658PU-N | NAGK (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP13659PU-N | NAGK (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300113 | NAGK |
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| Recombinant protein of human N-acetylglucosamine kinase (NAGK) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR216982L2V | Lenti ORF particles, Nagk (GFP-tagged) - Mouse N-acetylglucosamine kinase (Nagk), transcript variant 1, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205154L2V | Lenti ORF particles, Nagk (GFP-tagged) - Mouse N-acetylglucosamine kinase (Nagk), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR216982L1V | Lenti ORF particles, Nagk (Myc-DDK-tagged) - Mouse N-acetylglucosamine kinase (Nagk), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
| MR205154L1V | Lenti ORF particles, Nagk (Myc-DDK-tagged) - Mouse N-acetylglucosamine kinase (Nagk), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||