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NALP3 antibody

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Principal name

NALP3 antibody

Alternative names for NALP3 antibody

NLRP3, C1orf7, CIAS1, PYPAF1, NACHT, LRR and PYD domains-containing protein 3

SwissProt ID

Q8R4B8 (Mouse), Q96P20 (Human)

Gene ID

114548 (NLRP3), 216799 (Nlrp3)

Ncbi ID

9606, NP_001120934, NP_001073289.1, NP_004886.3, NM_001127461, NP_665826.1, NM_145827, NM_001079821, NM_004895, NM_183395, NM_001127462

Available reactivities

Hu (Human), Bov (Bovine), Rt (Rat), Can (Canine), Ms (Mouse), Por (Porcine), Prim (Primate)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P)

Background of NALP3 antibody

NLRP3/LP3 belongs to the NLR family of inte immune cytoplasmic receptors (reviewed in De rdo and Latz, 2011; Kanneganti, 2010; And, 2011). NLR family members contain an N-terminus effector region consisting of either a pyrin (PYD), a CARD, or a BIR domain. NLRs also have an intermediate conserved NOD domain and a C-terminus LRR domain. NLRs, like TLRs, alert the immune system to the presence of PAMPs and DAMPs. Like a number of other NLRs, NLRP3 functions in a multiprotein complex called the inflammasome. The inclusion of a particular NLR within an inflammasome depends upon the upstream trigger. The NLRP3 inflammasome is considered to be a global sensor and assembles in response to a broad spectrum of PAMPs and DAMPs. For example, helmith, bacteria, plasmodium and viruses have all been shown to activate NLRP3 (reviewed in Menu and Vince, 2011). The PYD of activated NLRP3 associates with the PYD of ASC, triggering the CARD of ASC to associate with the CARD of caspase-1 and completes the assembly of the NLRP3 inflammasome. This serves as a platform for caspase-1 activation. Activation of NLRP3 can be inhibited by glybenclamide (also known as glyburide) (reviewed in Lamkanfi et al, 2009). Glybenclamide is an ATP-sensitive potassion channel inhibitor that has been shown to inhibit various biological processes including activation of the NRLP3 inflammasome. Glybenclamide is thought to act downstream of the P2X7 receptor but upsteam of NLRP3. Glybenclamide blocks activation of caspase-1 and IL-1beta K+ efflux inhibition. Glybenclamide has a number of additiol functions besides blocking including enhancing insulin action and stimulating synthesis of glucose transporters. Researchers are encouraged to consult the published literature regarding additiol information about how glybenclamide perturbs biological processes.

General readings

1. Hoffman HM, Mueller JL, Broide DH, Wanderer AA, Kolodner RD. Mutation of a new gene encoding a putative pyrin-like protein causes familial cold autoinflammatory syndrome and Muckle-Wells syndrome. Nat Genet. 2001 Nov;29(3):301-5.
PMID: 11687797

H00114548-M01 - NALP3
Western Blot analysis of CIAS1 expression in transfected 293T cell line by CIAS1 monoclonal antibody (M01), clone 3B1.Lane 1: CIAS1 transfected lysate(118.2 KDa).Lane 2: Non-transfected lysate.
H00114548-B01P - NALP3
Western Blot analysis of NLRP3 expression in transfected 293T cell line (H00114548-T01) by NLRP3 MaxPab polyclonal antibody.Lane 1: CIAS1 transfected lysate(113.96 KDa).Lane 2: Non-transfected lysate.
H00114548-D01P - NALP3
Western Blot analysis of NLRP3 expression in transfected 293T cell line (H00114548-T02) by NLRP3 MaxPab polyclonal antibody.Lane 1: NLRP3 transfected lysate(118.20 KDa).Lane 2: Non-transfected lysate.
MAB3723 - NALP3
Immunofluorescence microscopy showing the detection of endogenous NLRP3 in THP-1 cells with NLRP3 monoclonal antibody, clone Nalpy3-a ( Cat # MAB3723 ).
AP18137PU-N - NALP3
Western blot analysis of NLRP3 Antibody (N-term) (AP18137PU-N) in MDA-MB231 cell line lysates (35ug/lane).NLRP3 (arrow) was detected using the purified Pab.
AP22372PU-N - NALP3
AP22372PU-N NLRP3 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Peripheral Blood Leukocytes at 2.5 µg/ml using Biotinylated anti-Goat IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.
AP32694PU-N - NALP3
AP32694PU-N (2 µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
TA309046 - NALP3
Sample(30 ug of whole cell lysate). A:Raji. 7.5% SDS PAGE. TA309046 diluted at 1:1500
TA323326 - NALP3
Predicted cell location: Cytoplasm. Positive control: Human esophagus and thyroid cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using NLRP3 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200)
TA323326 - NALP3
Predicted cell location: Cytoplasm. Positive control: Human esophagus and thyroid cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using NLRP3 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200)
TA336883 - NALP3
Western Blot: NALP3 Antibody (25N10E9) [TA336883] - Analysis using the NALP3 monoclonal antibody. (A), (B), mouse RAW lysate © and the recombinant mouse NLRP3 NOD domain protein immunogen (D) probed with 2 ug/ml antibody. goat anti-mouse Ig HRP s
TA336883 - NALP3
Western Blot: NALP3 Antibody (25N10E9) [TA336883] - Western blot analysis of Raw 264 cell lysate using NALP3 antibody (TA336883) at 2 ug/ml.
TA336895 - NALP3
Western Blot: NALP3 Antibody [TA336895] - Analysis of NALP3 using NALP3 antibody. Human testis lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat testis probed with NALP3 antibody at 5, 2 and 2 ug/ml respectively. goat anti-r
TA336895 - NALP3
Western Blot: NALP3 Antibody [TA336895] - Analysis of NALP3 using NALP3 antibody. Lane A) human NALP3 transfected cell lysate this antibody , B) mouse NALP3 transfected cell lysate this antibody , and C) HEK293 control lysate probed with NALP3 antibody at
TA351109 - NALP3
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using (NLRP3 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
TA351109 - NALP3
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (NLRP3 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
TA349228 - NALP3
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: K562 cells, hela cells, Primary antibody: (NLRP3 Antibody) at dilution 1/88, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes

16 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NALP3 antibody
AP32694PU-N (2 µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P, WB
0.1 mg / €380.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Western blotting.

NALP3 antibody
  Rabbit polyclonal antibody to NLRP3 (NLR family, pyrin domain containing 3)  
Sample(30 ug of whole cell lysate). A:Raji. 7.5% SDS PAGE. TA309046 diluted at 1:1500 Rabbit IgG Aff - Purified Hu WB
0.1 ml / €415.00
  OriGene Technologies, Inc.

NALP3 antibody
  Anti-NLRP3 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human esophagus and thyroid cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using NLRP3 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

NALP3 antibody
  Mouse Monoclonal NLRP3/NALP3 Antibody (25N10E9)  
Western Blot: NALP3 Antibody (25N10E9) [TA336883] - Analysis using the NALP3 monoclonal antibody. (A), (B), mouse RAW lysate © and the recombinant mouse NLRP3 NOD domain protein immunogen (D) probed with 2 ug/ml antibody. goat anti-mouse Ig HRP s Mouse IgG2b 25N10E9 Purified Hu, Ms WB
0.1 mg / €395.00
  OriGene Technologies, Inc.
+1 additional image

NALP3 antibody
  Rabbit Polyclonal NLRP3/NALP3 Antibody  
Western Blot: NALP3 Antibody [TA336895] - Analysis of NALP3 using NALP3 antibody. Human testis lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat testis probed with NALP3 antibody at 5, 2 and 2 ug/ml respectively. goat anti-r Rabbit Purified Hu, Ms, Prim, Rt WB
0.1 mg / €465.00
  OriGene Technologies, Inc.
+1 additional image

NALP3 antibody
  Rabbit Polyclonal Anti-NLRP3 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: K562 cells, hela cells, Primary antibody: (NLRP3 Antibody) at dilution 1/88, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes Rabbit IgG Purified Hu E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

NALP3 antibody
  Rabbit Polyclonal Anti-NLRP3 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using (NLRP3 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

NALP3 (N-term) antibody
Western blot analysis of NLRP3 Antibody (N-term) (AP18137PU-N) in MDA-MB231 cell line lysates (35ug/lane).NLRP3 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

NALP3 (C-term) antibody
AP22372PU-N Flow cytometric analysis of paraformaldehyde fixed human peripheral blood monocytes (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. NB: Gated on Granulocytes. Goat Aff - Purified Bov, Hu, Rt E, F, ICC/IF
0.1 mg / €380.00
  OriGene Technologies GmbH
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

NLRP3 - human gene knockout kit via CRISPR
NLRP3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NLRP3 - human gene knockout kit via CRISPR
NLRP3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NLRP3 - human gene knockout kit via CRISPR
NLRP3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NLRP3 - human gene knockout kit via CRISPR
NLRP3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

NLRP3 overexpression lysate
NLRP3 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, NLRP3 (mGFP-tagged)-Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 2, 200 uL, >10^7 TU/mL
Lenti ORF particles, NLRP3 (mGFP-tagged)-Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €2,040.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, NLRP3 (mGFP-tagged) - Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 4, 200 uL, >10^7 TU/mL
Lenti ORF particles, NLRP3 (mGFP-tagged) - Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €2,130.00
  OriGene Technologies, Inc.
 

16 Item(s)

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16 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. CRISPR (4)
  2. Human Lenti ORF Particle (2)
  3. Lysate (1)
  4. Primary Antibody (9)
Clonality
  1. Polyclonal (8)
  2. Monoclonal (1)
Target
  1. NALP3 (9)
Property
  1. C-term (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (9)
  2. Mouse (Ms) (5)
  3. Rat (Rt) (5)
  4. Bovine (Bov) (2)
  5. Canine (Can) (1)
  6. Porcine (Por) (1)
  7. Primate (Prim) (1)
Applications
  1. Western blot / Immunoblot (WB) (6)
  2. Enzyme Immunoassay (E) (5)
  3. Paraffin Sections (P) (3)
  4. Flow cytometry (F) (1)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
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  2. Aff - Purified (3)
Host
  1. Rabbit (6)
  2. Goat (2)
  3. Mouse (1)
Images
  1. 1 image (9)
Images: Applications
  1. Western blot / Immunoblot (WB) (6)
  2. IHC (2)
  3. Flow cytometry (F) (1)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Species (CRISPR)
  1. Human (4)
Donor Vector
  1. GFP-Puro (1)
  2. Luciferase-Puro (1)
  3. RFP-BSD (1)
  4. mBFP-Neo (1)
Manufacturer
  1. OriGene Technologies (16)
Service
  • OriGene Technologies GmbH
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  • Germany
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