NANOG antibody
Principal name
NANOG antibody
Alternative names for NANOG antibody
Homeobox protein NANOG
Ncbi ID
NP_079141, NP_079141.2, 9606, 10090, ABZ92376.1, NP_082292.1, NM_024865
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP), Dot blot (Dot), Frozen Sections (C)
Background of NANOG antibody
Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
General readings
1. Raya A et.al. Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells. Nature. 2009 Jul 2;460(7251):53-9. PMID: 19483674
2. Montserrat N, Garreta E, González F, Gutiérrez J, Eguizábal C, Ramos V, Borrós S, Izpisua Belmonte JC. Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system.
J Biol Chem. 2011 Apr 8;286(14):12417-28. PMID: 21285354
3. Montserrat N, Bahima EG, Batlle L, Häfner S, Rodrigues AM, González F, Belmonte JC.
Generation of pig iPS cells: a model for cell therapy. J Cardiovasc Transl Res. 2011 Apr;4(2):121-30. PMID: 21088946
4. Rodríguez-Pizà I, Richaud-Patin Y, Vassena R, González F, Barrero MJ, Veiga A, Raya A, Belmonte JC. Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions.
Stem Cells. 2010 Jan;28(1):36-44. PMID: 19890879
5. Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, Vassena R, Bilić J, Pekarik V, Tiscornia G, Edel M, Boué S, Izpisúa Belmonte JC. Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol. 2008 Nov;26(11):1276-84. PMID: 18931654
6. Montserrat N, Ramírez-Bajo MJ, Xia Y, Sancho-Martinez I, Moya-Rull D, Miquel-Serra L, Yang S, Nivet E, Cortina C, González F, Izpisua Belmonte JC, Campistol JM. Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, oct4 and sox2. J Biol Chem. 2012 Jul 13;287(29):24131-8. PMID: 22613719
Human Jurkat; WB Suggested Anti-NANOG Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; NANOG antibody - N-terminal region (P100591_P050) in Human Jurkat cells using Western Blot
NANOG monoclonal antibody (M01), clone 2C11 Western Blot analysis of NANOG expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged NANOG is approximately 0.03ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NANOG is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NANOG is approximately 0.03ng/ml as a capture antibody.
NANOG monoclonal antibody (M09), clone 1F8 Western Blot analysis of NANOG expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged NANOG is approximately 0.3ng/ml as a capture antibody.
Human Jurkat; WB Suggested Anti-NANOG Antibody. . Titration: 1.25 ug/ml. . Positive Control: Jurkat Whole Cell; NANOG antibody - N-terminal region (P100591_T100) in Human Jurkat cells using Western Blot
Western Blot (Reduced) using NANOG Antibody Cat.-No AP01140BT
Western Blot (Unreduced) using NANOG Antibody Cat.-No AP01140BT
Direct ELISA using NANOG Antibody Cat.-No AP01140BT
Sandwich ELISA using NANOG Antibody Cat.-No AP01140BT
Formalin-Fixed, Paraffin-Embedded Sections of human prostate malignant adenocarcinoma stained with NANOG Antibody Cat.-No AP01140PU
Western Blot (Unreduced) using NANIOG Antibody Cat.-No AP01140PU
Western Blot (Reduced) using NANIOG Antibody Cat.-No AP01140PU
Sandwich ELISA using NANOG Antibody Cat.-No AP01140PU
Western blot analysis of NANOG in ( A ) HeLa and ( B ) NIH/3T3 cell lysate using NANOG polyclonal antibody ( Cat # PAB0375 ) at 2 µg/ml.
Western blot analysis of NANOG ( arrow ) using NANOG polyclonal antibody ( Cat # PAB1886 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the NANOG gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of NANOG polyclonal antibody ( Cat # PAB1886 ) in K-562 cell line lysates ( 35 µg/lane ) . NANOG ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Formalin-fixed and paraffin-embedded human testis tissue reacted with NANOG polyclonal antibody ( Cat # PAB1887 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of NANOG ( arrow ) using NANOG polyclonal antibody ( Cat # PAB1887 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the NANOG gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of NANOG polyclonal antibody ( Cat # PAB1887 ) in HeLa cell line lysates ( 35 µg/lane ) . NANOG ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
NANOG polyclonal antibody ( Cat # PAB6837 ) ( 5ug/ml ) staining ( green ) parts of a colony of induced pluriform stem cells derived from human keratinocytes. Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain.
NANOG polyclonal antibody ( Cat # PAB6837 ) ( 0.03 µg/ml ) staining of human ovary lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
NANOG polyclonal antibody ( Cat # PAB7232 ) ( 2µg/ml ) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
NANOG polyclonal antibody ( Cat # PAB7232 ) ( 0.05 µg/ml ) staining of HeLa ( nuclear ) cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Human Jurkat; WB Suggested Anti-NANOG Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; NANOG antibody - N-terminal region (ARP47988_P050) in Human Jurkat cells using Western Blot
Immunohistochemistry: Paraffin Embedded Sections of Human seminoma tissue were incubated with anti-Human Nanog (1/50) for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Western blot analysis: The cell lysates of NIH3T3 (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue using anti-NANOG monoclonal antibody Cat.-No AM11049SU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NANOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Western Blot analysis of anti-NANOG monoclonal antibody Cat.-No AM11049SU-N by NONAG recombinant protein. NANOG (NANOG + His tag)(arrow) was detected using the Mab.
Western blot analysis of anti-NANOG monoclonal antibody Cat.-No AM11049SU-N in K562 cell line lysates using (35ug/lane). This demonstrates the NANOG antibody detected the NANOG protein (arrow).
The cell lysate of NIH/3T3 ( 35 µg ) was resolved by SDS-PAGE and probed with NANOG monoclonal antibody, clone 5A10 ( Cat # MAB2042 ) ( 1:500 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence with Nanog polyclonal antibody ( Cat # PAB13693 ). Feeder cells are indicated by dotted line circle.
Western blot analysis from mouse embryonic stem ( ES ) cells with Nanog polyclonal antibody ( Cat # PAB13693 ).
Immunofluorescence with NANOG polyclonal antibody ( Cat # PAB13694 ). Human embryonic stem cells, grown on mouse embryonic fibroblasts. Nuclear staining by DAPI.
Western blot with NANOG polyclonal antibody ( Cat # PAB13694 ) and ACTB monoclonal antibody. Each lane using 20 µg protein.
Human Fetal Kidney; NANOG (Nanog homeobox) Antibody (against the middle region of NANOG) (50ug) validated by WB using Fetal kidney lysate at 0.2-1 ug/ml.; NANOG Antibody - middle region (P100633_P050) in Human Fetal Kidney cells using Western Blot
Human OVCAR-3; WB Suggested Anti-NANOG Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: OVCAR-3 cell lysate. NANOG is supported by BioGPS gene expression data to be expressed in OVCAR3.; NANOG antibody - N-terminal region (P100728_P050) in Human OVCAR-3 cells using Western Blot
Human Testis (formalin-fixed, paraffin-embedded) stained with NANOG antibody AP22506PU-N at 2 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Antibody (0.05 µg/ml) staining of HeLa (nuclear) cell lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Human Spleen (formalin-fixed, paraffin-embedded) stained with NANOG antibody AP22771PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Testis (formalin-fixed, paraffin-embedded) stained with NANOG antibody AP22771PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunofluorescence analysis of NANOG in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
(LEFT)Western blot analysis of NANOG in Hela cell line lysates (35 µg/lane). (RIGHT)Western blot analysis of NANOG in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2).
Western blot analysis of extracts from human Umbilical cord mesenchymal stem cell using Nanog Antibody AP26063PU-N.
Recombinant human NANOG 1-154aa, His-tagged
Recombinant human Nanog, 1-305 aa
Confocal Immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog antibody Cat.-No AM06293SU-N (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Western blot analysis using NANOG antibody Cat.-No AM06293SU-N against NTERA-2 cell lysate (2).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-NANOG rabbit polyclonal antibody. (TA590448)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NANOG rabbit polyclonal antibody. (TA590448)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-NANOG rabbit polyclonal antibody. (TA590448)
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-NANOG rabbit polyclonal antibody. (TA590448)
Immunohistochemical staining of human bronchus shows strong nuclear positivity in subsets of respiratory epithelial cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line A-431 shows positivity in nucleus & nucleoli.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of HeLa cells using anti-NANOG rabbit polyclonal antibody (TA590448).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NANOG (RC210243, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NANOG.
Western Blot: NANOG Antibody - Cell lysate from 293 over expressing Nanog was subjected to SDS-PAGE and transfer to nitrocellulose membrane. The membrane was incubated with anti-Nanog antibody (1:1000 dilution) and then probed anti-rabbit IgG-HRP. Signals were acquired with a CCD camera. Arrow points to unmodified Nanog. The band above the arrow is non-specific. Data courtesy of a SDI customer.
ELISA: NANOG Antibody
Western blot analysis of anti-NANOG Antibody (N-term) (Cat.#TA302153) in K562 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (N-term) (Cat.#TA302153). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody (N-term)(Cat.#TA302153), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF image of SY5Y cells stained with TA302153 NANOG (N-term) antibody. SY5Y cells were incubated with TA302153 NANOG (N-term) primary antibody (1:500, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin . Nuclei were counterstained with Hoechst 33342 (blue). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.
IF image of Hela cell stained with NANOG Antibody (N-term)(Cat#TA302153).Hela cells were incubated with NANOG primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . NANOG immunoreactivity is localized to Nucleus significantly.
NANOG Antibody (N-term) (Cat. #TA302153) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NANOG Antibody (Center) (Cat. #TA302154) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NANOG antibody detected the NANOG protein (arrow).
Formalin-fixed and paraffin-embedded human Testis tissue reacted with NANOG antibody (Center)(Cat.#TA302154), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescence analysis of NANOG Antibody (Center) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
Flow cytometric analysis of hela cells using NANOG Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA303048 (0.03µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA303206 (0.05µg/ml) staining of HeLa (nuclear) cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of NANOG in human spleen tissue lysate with NANOG antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry of NANOG in human spleen tissue with NANOG antibody at 5 ug/mL.
Immunofluorescence of NANOG in Human Spleen cells with NANOG antibody at 20 ug/mL.
NTERA cell line lysate was loaded at 1x10e5 cells/lane, probed with 1 ug/mL of Anti-Human Nanog Purified and revealed with Anti-Mouse IgG HRP.
Predicted band size: 35 kDa . Positive control: Human Umbilical cord mesenchymal stem cell lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE All lane: Human Umbilical cord mesenchymal stem cell Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of NANOG (arrow) using rabbit NANOG Antibody (S285) (Cat.# TA324577). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
Western blot analysis of NANOG Antibody (S285) (Cat.# TA324577) in HepG2 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.
IF image of SY5Y cells stained with TA324577 NANOG (S285) antibody. SY5Y cells were incubated with TA324577 NANOG (S285) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin . Nuclei were counterstained with Hoechst 33342 (blue). Nanog immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.
This antibody stained formalin-fixed, paraffin-embedded sections of human prostate malignant adenocarcinoma. The recommended concentration is 0.5 mg/ml with an overnight incubation at 4C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
TA334190 (0.5µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot detection of Nanog in 20 ug of human hippocampus tissue lysate (lanes 2 and 4) with Nanog polyclonal at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. MW marker lane 1. Peptide absorption control lane 3.
Western Blot: Nanog Antibody [TA336515] - Western blot detection of Nanog in 20 ug of human hippocampus tissue lysate (lanes 2 and 4) with Nanog polyclonal at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. MW marker lane 1. Peptid
Western Blot: Nanog Antibody (1E6C4) [TA336771] - Western blot analysis using anti-Nanog monoclonal antibody against NTERA-2 cell lysate (lane 2).
Immunocytochemistry/Immunofluorescence: Nanog Antibody (1E6C4) [TA336771] - Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using anti-Nanog Mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloid
Immunohistochemistry-Paraffin: Nanog Antibody [TA336902] - IHC staining of Nanog in mouse testis using DAB with hematoxylin counterstain.
Western Blot: Nanog Antibody [TA337087] - Western blot analysis of Nanog in (A) HeLa and (B) NIH 3T3 cell lysate using TA337087 at 2 ug/ml.
Immunocytochemistry/Immunofluorescence: Nanog Antibody [TA337087] - Nanog antibody was tested in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. An antibody d
Total cell lysate from Ntera-2 cells (lane 1) and HeLacells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-NANOG (clone 16H3A48) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
Ntera-2 cells (left) and HeLa cells (right) were stained with purified anti-NANOG (clone 16H3A48) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody and Alexa Fluor 594™ conjugated phalloidin (red).
Western blot analysis of NTERA-2 (lane 1), NF-1 (lane 2), and Hela (lane 3) using anti-Nanog antibody (clone 23D2-3C6). Anti-ß actin antibody (poly6221) was used as a loading control.
NTERA-2 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and then blocked with 5% FBS for 30 minutes. The cells were then intracellularly stained with 5 µg/ml anti-Nanog (clone 23D2-3C6) in a blocking buffer overnight at 4°C and followed by DyLight™ 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA336902 | NANOG antibody |
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| Rabbit Polyclonal Nanog Antibody | |||||||
![Immunohistochemistry-Paraffin: Nanog Antibody [TA336902] - IHC staining of Nanog in mouse testis using DAB with hematoxylin counterstain.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-13177-1-H.jpg) |
Rabbit | Purified | Ms | C, ICC/IF, P |
0.1 ml /
€395.00
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| OriGene Technologies, Inc. | |||||||