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NANOS1 antibody

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Principal name

NANOS1 antibody

Alternative names for NANOS1 antibody

NOS1, Nanos homolog 1

SwissProt ID

Q80WY3 (Mouse), Q8WY41 (Human)

Gene ID

340719 (NANOS1), 332397 (Nanos1)

Ncbi ID

NP_955631, 9606, NP_955631.1, NM_199461, Q8WY41, NP_848508.2

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NANOS1 antibody

Nanos1 is one of three known mammalian homologs to the Drosophila gene nanos. Nanos1 is an RNA-binding protein containing a zinc-finger motif and is expressed in the developing nervous system and continues in the adult brain. Interestingly, unlike mice deficient in either nanos2 or nanos3, mice lacking the nanos1 gene develop normally with no sign of abnormalities. Recently it has been found that expression of nanos1 mRNA is down-regulated by E-cadherin in a human breast cancer cell line and the amino-terminal domain on Nanos1 interacts with the E-cadherin-binding protein p120ctn. Furthermore, overexpression of Nanos1 in human colorectal DLD1 cancer cells functionally abolished cell-cell adhesion, allowing the cancer cells to develop strong migratory and invasive properties. These results suggest that targeting Nanos1 might prove an effective strategy in the treatment of E-cadherin-negative tumors.

General readings

Jaruzelska J, Kotecki M, Kusz K, Spik A, Firpo M, Reijo Pera RA. Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells. Dev Genes Evol. 2003 Apr;213(3):120-6.

ARP41208_T100 - NANOS1
Human HepG2; WB Suggested Anti-NANOS1 Antibody Titration: 5.0ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate; NANOS1 antibody - middle region (ARP41208_T100) in Human HepG2 cells using Western Blot
PAB2486 - NANOS1
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 polyclonal antibody ( Cat # PAB2486 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
PAB2486 - NANOS1
Western blot analysis of NANOS1 polyclonal antibody ( Cat # PAB2486 ) in T-47D cell line lysates ( 35 µg/lane ) . NANOS1 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
PAB6760 - NANOS1
NANOS1 polyclonal antibody ( Cat # PAB6760 ) ( 0.3 µg/ml ) staining of human testis lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
PAB13390 - NANOS1
Western blot analysis of NANOS1 in SK-N-SH cell lysate with NANOS1 polyclonal antibody ( Cat # PAB13390 ) at 1 µg/ml in ( A ) the presence and ( B ) the absence of blocking peptide.
PAB13391 - NANOS1
Western blot analysis of NANOS1 in rat brain tissue lysate with NANOS1 polyclonal antibody ( Cat # PAB13391 ) at ( A ) 1 and ( B ) 2 µg/ml.
ARP41209_P050 - NANOS1
Human 293T; WB Suggested Anti-NANOS1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 293T cell lysate; NANOS1 antibody - middle region (ARP41209_P050) in Human 293T cells using Western Blot
TA303006 - NANOS1
TA303006 (0.3µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA306605 - NANOS1
Western blot analysis of Nanos1 in SK-N-SH cell lysate with Nanos1 antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide.
TA306605 - NANOS1
Immunohistochemistry of Nanos1 in human brain tissue with Nanos1 antibody at 2.5 ug/ml
TA306605 - NANOS1
Immunofluorescence of nanos1 in human brain tissue with nanos1 antibody at 20 ug/mL.
TA306606 - NANOS1
Western blot analysis of Nanos1 in rat brain tissue lysate with Nanos1 antibody at (A) 1 and (B) 2 µg/ml.
TA306606 - NANOS1
Immunofluorescence of nanos1 in human brain tissue with nanos1 antibody at 20 ug/mL.
TA324534 - NANOS1
Western blot analysis of anti-NANOS1 Antibody (C-term) (RB13839) in T47D cell line lysates (35ug/lane). NANOS1 (arrow) was detected using the purified Pab.
TA324534 - NANOS1
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 antibody (C-term) (Cat.#TA324534), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
TA324534 - NANOS1
Immunofluorescence analysis of anti-NANOS1 Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
TA324534 - NANOS1
Flow cytometric analysis of hela cells using Nanos Homologue 1 (NANOS1) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA344041 - NANOS1
WB Suggested Anti-NANOS1 Antibody Titration: 5.0 ug/ml; ELISA Titer: 1: 12500; Positive Control: HepG2 cell lysate
TA344042 - NANOS1
WB Suggested Anti-NANOS1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 293T cell lysate
TA345723 - NANOS1
Host: Rabbit; Target Name: NANOS1; Sample Tissue: Thymus Tumor lysates; Antibody Dilution: 1.0 ug/ml

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NANOS1 antibody
  Goat Polyclonal Antibody against NANOS1  
TA303006 (0.3µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

NANOS1 (N-term) antibody
  Rabbit Polyclonal Nanos1 Antibody  
Western blot analysis of Nanos1 in SK-N-SH cell lysate with Nanos1 antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

NANOS1 antibody
  Rabbit Polyclonal Nanos1 Antibody  
Western blot analysis of Nanos1 in rat brain tissue lysate with Nanos1 antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+1 additional image

NANOS1 (C-term) antibody
  Rabbit polyclonal Nanos Homologue 1 (NANOS1) Antibody (C-term)  
Western blot analysis of anti-NANOS1 Antibody (C-term) (RB13839) in T47D cell line lysates (35ug/lane). NANOS1 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu ICC/IF, P, WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+3 additional images

Lysates

Catalog No.    

NANOS1 overexpression lysate
NANOS1 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

5 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (4)
Clonality
  1. Polyclonal (4)
Target
  1. NANOS1 (4)
Property
  1. C-term (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (1)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Enzyme Immunoassay (E) (2)
  3. Paraffin Sections (P) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Aff - Purified (3)
  2. Purified (1)
Host
  1. Rabbit (3)
  2. Goat (1)
Images
  1. 1 image (4)
  2. 3+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Immunofluorescence (IF) (3)
  3. IHC (2)
Manufacturer
  1. OriGene Technologies (5)
Service
  • OriGene Technologies GmbH
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