NANP antibody
Principal name
NANP antibody
Alternative names for NANP antibody
C20orf147, HDHD4, N-acylneuraminate-9-phosphatase
Ncbi ID
9606, NP_689880, NP_689880.1, NM_152667, NP_080362.1, NM_026086, NM_001009409
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P)
Background of NANP antibody
- Kit Component:
- - KN310723G1, Nanp gRNA vector 1 in pCas-Guide vector
- KN310723G2, Nanp gRNA vector 2 in pCas-Guide vector
- KN310723D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Hao J., et al. (2006) Biochem J. 397: 195-201.
Maliekal P., et al. (2006) Glycobiology. 16:165-172.
Immunofluorescence of purified MaxPab antibody to NANP on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of NANP expression in transfected 293T cell line (H00140838-T01) by NANP MaxPab polyclonal antibody.Lane 1: NANP transfected lysate(27.28 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab antibody to NANP on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of NANP expression in transfected 293T cell line (H00140838-T01) by NANP MaxPab polyclonal antibody.Lane 1: NANP transfected lysate(27.28 KDa).Lane 2: Non-transfected lysate.
Human Jurkat; WB Suggested Anti-NANP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; NANP antibody - middle region (ARP53100_P050) in Human Jurkat cells using Western Blot
Recombinant human NANP, 1-248aa, His-tagged
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NANP (RC205503, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NANP.
Anti-NANP mouse monoclonal antibody (TA504539) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NANP(RC205503).
Immunofluorescent staining of HeLa cells using anti-NANP mouse monoclonal antibody (TA504539).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NANP (RC205503, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NANP.
Flow cytometric Analysis of Hela cells, using anti-NANP antibody(TA504539),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-NANP antibody(TA504539),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NANP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504547)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NANP (RC205503, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NANP.
Anti-NANP mouse monoclonal antibody (TA504557) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NANP(RC205503).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NANP (RC205503, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NANP.
Anti-NANP mouse monoclonal antibody (TA504579) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NANP(RC205503).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NANP (RC205503, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NANP.
Flow cytometric Analysis of Jurkat cells, using anti-NANP antibody(TA504579),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NANP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504580)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NANP (RC205503, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NANP.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NANP (RC205503, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NANP.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NANP monoclonal antibody at 1:2000 dilution (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NANP (RC205503, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NANP.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NANP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504598)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NANP (RC205503, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NANP.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NANP monoclonal antibody at 1:2000 dilution (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
WB Suggested Anti-NANP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate
Anti-NANP antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NANP antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NANP antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NANP antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA504539 | NANP antibody |
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| NANP mouse monoclonal antibody, clone OTI4D11 (formerly 4D11) | |||||||
 |
Mouse | IgG2a | OTI4D11 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
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| TA341076 | NANP antibody |
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| Rabbit Polyclonal Anti-NANP Antibody (N-Terminus) | |||||||
 |
Rabbit | Purified | Hu, Por |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TA341079 | NANP (Internal) antibody |
||||||
| Rabbit Polyclonal Anti-NANP Antibody (Internal) | |||||||
 |
Rabbit | Aff - Purified | Bear, Bov, Bt, Can, Chk, Elephant, Hst, Hu, L, Ms, Op, Platypus, Por, Rb, Rt, Tr | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341080 | NANP (N-term) antibody |
||||||
| Rabbit Polyclonal Anti-NANP Antibody (N-Terminus) | |||||||
|
Rabbit | Aff - Purified | Bear, Bov, Bt, Can, Chk, Elephant, Hst, Hu, Mky, Ms, Platypus, Por, Prim, Rt, Tr | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341081 | NANP (Internal) antibody |
||||||
| Rabbit Polyclonal Anti-NANP Antibody (Internal) | |||||||
|
Rabbit | Aff - Purified | Bear, Bov, Bt, Can, Hu, Mky, Ms, Por, Prim, Rb, Rt | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA504537 | NANP antibody |
||||||
| NANP mouse monoclonal antibody, clone OTI2F2 (formerly 2F2) | |||||||
 |
Mouse | IgG2a | OTI2F2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA504547 | NANP antibody |
||||||
| NANP mouse monoclonal antibody, clone OTI4D5 (formerly 4D5) | |||||||
 |
Mouse | IgG2a | OTI4D5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA504557 | NANP antibody |
||||||
| NANP mouse monoclonal antibody, clone OTI2C2 (formerly 2C2) | |||||||
 |
Mouse | IgG2a | OTI2C2 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA504579 | NANP antibody |
||||||
| NANP mouse monoclonal antibody, clone OTI1G8 (formerly 1G8) | |||||||
 |
Mouse | IgG2a | OTI1G8 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA504580 | NANP antibody |
||||||
| NANP mouse monoclonal antibody, clone OTI3C1 (formerly 3C1) | |||||||
 |
Mouse | IgG2a | OTI3C1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA504581 | NANP antibody |
||||||
| NANP mouse monoclonal antibody, clone OTI2A2 (formerly 2A2) | |||||||
 |
Mouse | IgG2a | OTI2A2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA504597 | NANP antibody |
||||||
| NANP mouse monoclonal antibody, clone OTI2D11 (formerly 2D11) | |||||||
 |
Mouse | IgG2b | OTI2D11 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA504598 | NANP antibody |
||||||
| NANP mouse monoclonal antibody, clone OTI4F5 (formerly 4F5) | |||||||
 |
Mouse | IgG2b | OTI4F5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305503 | NANP |
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| Recombinant protein of human N-acetylneuraminic acid phosphatase (NANP) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY407388 | NANP overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203153L2V | Lenti ORF particles, Nanp (GFP-tagged) - Mouse N-acetylneuraminic acid phosphatase (Nanp), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203153L1V | Lenti ORF particles, Nanp (Myc-DDK-tagged) - Mouse N-acetylneuraminic acid phosphatase (Nanp), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR202388L2V | Lenti ORF particles, Nanp (GFP-tagged ORF) - Rat N-acetylneuraminic acid phosphatase (Nanp), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR202388L1V | Lenti ORF particles, Nanp (Myc-DDK-tagged ORF) - Rat N-acetylneuraminic acid phosphatase (Nanp), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||