NCBP2 antibody
Principal name
NCBP2 antibody
Alternative names for NCBP2 antibody
CBP20, PIG55, Nuclear cap-binding protein subunit 2
Ncbi ID
9606, NP_001036005.1, NP_001036005, NM_007362, NP_031388, NP_080830.1, NM_026554, NM_001042540
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)
Background of NCBP2 antibody
In eukaryotes, the majority of mRNAs have an m(7)G cap, which is added cotranscriptionally and plays a critical role in many aspects of mRNA metabolism. The effect of the cap on translation is mediated by the initiation factor eIF-4F, whereas the effect on pre-mRNA splicing involves a nuclear complex (CBC). CBC consists of two cap binding proteins, CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation and U snRNA export. The genes CBC1 and CBC2 encode CBP80 and CBP20, respectively. CBP80 comprises three domains, each containing a MIF4G domain. CBP20 has an RNAP fold and associates with the second and third domains of CBP80. CBP also plays a role in nonsense-mediated decay (NMD), a process which eliminates mRNAs, and prematurely terminates translation. CBP80-bound mRNA undergoes a pioneer round of translation before CBP80-CBP20 are replaced by eIF4E, and Upf2 and Upf3 proteins.
General readings
Strasser A., et al.( (2005) EMBO J 24(13):2235-43.
Lane1:Hela whole cell lysate
Lane2:Mouse kidney tissue lysate
Lane3:Rat liver tissue lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315201 | NCBP2 antibody |
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| Rabbit polyclonal anti-NCBP2 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP06704PU-N | NCBP2 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301519 | NCBP2 (transcript variant 1) |
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| Recombinant protein of human nuclear cap binding protein subunit 2, 20kDa (NCBP2), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP321605 | NCBP2 (transcript variant 2) |
||||
| Purified recombinant protein of Homo sapiens nuclear cap binding protein subunit 2, 20kDa (NCBP2), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402134 | NCBP2 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR219175L2V | Lenti ORF particles, Ncbp2 (GFP-tagged) - Mouse nuclear cap binding protein subunit 2 (Ncbp2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR219175L1V | Lenti ORF particles, Ncbp2 (Myc-DDK-tagged) - Mouse nuclear cap binding protein subunit 2 (Ncbp2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
