NCK1 antibody
Principal name
NCK1 antibody
Alternative names for NCK1 antibody
Cytoplasmic protein NCK1, NCK, NCK adaptor protein 1, SH2/SH3 adaptor protein NCK-alpha
SwissProt ID
P16333 (Human)
Gene ID
4690 (NCK1)
Ncbi ID
9606, NP_006144, NP_001177725.1, NP_006144.1, NM_006153, NM_001190796
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)
Background of NCK1 antibody
- Kit Component:
- - KN202869G1, NCK1 gRNA vector 1 in pCas-Guide vector
- KN202869G2, NCK1 gRNA vector 2 in pCas-Guide vector
- KN202869D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Braverman. L.E. and Quilliam. L.A. (1999) J Biol Chem 274: 5542-5549 Latreille. M. and Larose. L. (2006) J Biol Chem 281: 26633-26644 Kremer. B.E., et al. (2007) Cell 130: 837-850
NCK1 monoclonal antibody (M01), clone 1A1 Western Blot analysis of NCK1 expression in HeLa NE ( Cat # L013V3 ).
Western Blot analysis of NCK1 expression in transfected 293T cell line by NCK1 monoclonal antibody (M01), clone 1A1.Lane 1: NCK1 transfected lysate(42.9 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged NCK1 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to NCK1 on HeLa cell. [antibody concentration 20 ug/ml]
Western Blot analysis of NCK1 expression in transfected 293T cell line (H00004690-T01) by NCK1 MaxPab polyclonal antibody.Lane 1: NCK1 transfected lysate(41.47 KDa).Lane 2: Non-transfected lysate.
NCK1 MaxPab polyclonal antibody. Western Blot analysis of NCK1 expression in A-431.
Proximity Ligation Analysis (PLA) of protein-protein interactions between NCK1 and ABL2. HeLa cells were stained with anti-NCK1 rabbit purified polyclonal 1:1200 and anti-ABL2 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of NCK1 expression in transfected 293T cell line (H00004690-T01) by NCK1 MaxPab polyclonal antibody.Lane 1: NCK1 transfected lysate(42.90 KDa).Lane 2: Non-transfected lysate.
Immunoblot analysis of cell lysates.Lane 1 : U937 cell lysate. Lnae 2 : A-431 cell lysate.Lane 3 : NIH/3H3 cell lysate.Lane 4 : C6 cell lysate.
Immunoblot analysis of cell lysates.Lane 1 : HeLa cell lysate. Lnae 2 : RD cell lysate.Lane 3 : PC-12 cell lysate.Lane 4 : NIH/3T3 cell lysate.
Western blot analysis of NCK1 Antibody (N-term) (AP18125PU-N) in Hela cell line lysates (35ug/lane). NCK1 (arrow) was detected using the purified Pab.
Recombinant human NCK1, 1- 377 aa, His-tagged
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NCK1 mouse mAb with DAB staining.
Western blot analysis using NCK1 mAb against human NCK1 (AA: 203-371) recombinant protein (Expected MW is 44.9 kDa)
Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.
Flow cytometric analysis of Jurkat cells using NCK1 mouse mAb (green) and negative control (red).
The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NCK1 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis of NCK1 in whole cell lysate of mouse lymph node lymphocytes using the antibody EM-06.
Predicted band size: 43 kDa . Positive control: Jurkat and K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: K562 cell lysate Lysates: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using NCK1 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted band size: 43 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using NCK1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x200)
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM06766PU-N | NCK1 antibody |
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Mouse | IgG1 | 5B7 | Purified | Hu, Mky | E, F, P, WB |
0.1 mg /
€410.00
|
| OriGene Technologies GmbH | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA324086 | NCK1 antibody |
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| Anti-NCK1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA324087 | NCK1 antibody |
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| Anti-NCK1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AM26753PU-N | NCK1 (2nd SH3 dom.) antibody |
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 |
Mouse | IgG1 | EM-06 | Purified | Hu, Ms | ICC/IF, WB |
0.1 mg /
€260.00
|
| OriGene Technologies GmbH | |||||||
| AP18125PU-N | NCK1 (N-term) antibody |
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 |
Rabbit | Hu | WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416832 | NCK1 overexpression lysate |
|
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY434154 | NCK1 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
