NCOR1 antibody
Principal name
NCOR1 antibody
Alternative names for NCOR1 antibody
N-CoR1, N-CoR,KIAA1047, Nuclear receptor corepressor 1
Gene ID
9611 (NCOR1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of NCOR1 antibody
Retinoids are metabolites of vitamin A (retinol) and are believed to represent important signaling molecules during vertebrate development and tissue differentiation.Two families of retinoid receptors have been identified. Retinoic acid receptors (RARs), include RARα, RARβ and RARγ, each of which have a high affinity for all-trans retinoic acids and belong to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor. Two cofactors that function to repress transcription, designated SMRT and N-CoR, have been shown to associate with the thyroid receptor and RAR in their unliganded state and are released from them upon ligand binding. The carboxy termini of both proteins contain receptor interacting domains while their amino termini contain two previously undescribed repressor domains. SMRT (silencing mediator for RARs and TRs) is 1495 amino acids in length with an estimated molecular weight of 168 kDa. N-CoR (nuclear receptor corepressor) is a protein 2453 amino acids in length with a relative molecular weight of 270 kDa.
General readings
Horlein,A.J., et al., (1995) Nature 377 (6548), 397-404
Human Intestine; NCOR1 antibody - N-terminal region (ARP32479_P050) in Human Intestine cells using Immunohistochemistry
Human Small Intestine; WB Suggested Anti-NCOR1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Small Intestine; NCOR1 antibody - N-terminal region (ARP32479_P050) in Human Small Intestine cells using Western Blot
Immunohistochemistry (IHC) analyzes of NCoR1 antibody (Cat.-No.: AP06449PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of NCoR1 antibody (Cat.-No.: AP06449PU-N) in extracts from MDA-MB-435 cells.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the NCOR1 polyclonal antibody ( Cat # PAB2352 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
The NCOR1 polyclonal antibody ( Cat # PAB2352 ) is used in Western blot to detect NCOR1 in mouse thymus tissue lysate.
Immunohistochemical analysis of paraffin-embedded human cerebra (left) and breast carcinoma tissue (right), showing nuclear location with DAB staining using NCOR1 antibody Cat.-No AM06272SU-N
Western blot analysis using NCOR1 NCOR1 antibody Cat.-No AM06272SU-N against truncated Trx-NCOR1 recombinant protein (Lane 1).
Immunohistochemical analysis of NCOR1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of NCOR1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of NCOR1 expression in K562 (A); HeLa (B) whole cell lysates.
Western blot analysis of extracts from MDA-MB-435 cells, using NCoR1 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using NCoR1 antibody.The picture on the right is treated with the synthesized peptide.
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human cervical and esophagus cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded Human cervical and esophagus cancer tissue using NCOR1 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human cervical and esophagus cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded Human cervical and esophagus cancer tissue using NCOR1 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human cervical cancer and tonsil tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer and tonsil tissue using NCOR1 antibody at dilution 1/100, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human cervical cancer and tonsil tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer and tonsil tissue using NCOR1 antibody at dilution 1/100, on the right is treated with the synthetic peptide. (Original magnification:x200)
The anti-NCOR1 Pab (Cat. #TA324390) is used in Western blot to detect NCOR1 in mouse thymus tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
IF image of Hela cell stained with (MOUSE) Ncor1 Antibody(Cat#TA324390). Hela cells were incubated with Ncor1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . Ncor1 immunoreactivity is localized to nucleus significantly.
Western blot analysis on MDA-MB-435 cell lysate using NCoR1 Antibody.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM06272SU-N | NCOR1 antibody |
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Mouse | IgG1 | 7A7A9 | Ascites | Hu | P, WB |
0.1 ml /
€420.00
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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