NDC80 antibody
Principal name
NDC80 antibody
Alternative names for NDC80 antibody
HEC, HEC1, KNTC2
SwissProt ID
O14777 (Human)
Gene ID
10403 (NDC80)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C)
Background of NDC80 antibody
- Kit Component:
- - KN207565G1, NDC80 gRNA vector 1 in pCas-Guide vector
- KN207565G2, NDC80 gRNA vector 2 in pCas-Guide vector
- KN207565D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Chen Y, Riley DJ, Chen PL, Lee WH.
HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis.
Mol Cell Biol. 1997 Oct;17(10):6049-56.
PMID: 9315664
KNTC2 monoclonal antibody (M01), clone 1A10 Western Blot analysis of KNTC2 expression in HeLa NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged KNTC2 is approximately 0.1ng/ml as a capture antibody.
NDC80 polyclonal antibody ( Cat # PAB6236 ) staining ( 0.5 µg/ml ) of HeLa lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot analysis of NDC80 expression in transfected 293T cell line (H00010403-T02) by NDC80 MaxPab polyclonal antibody.Lane 1: NDC80 transfected lysate(73.90 KDa).Lane 2: Non-transfected lysate.
Human Adrenal (formalin-fixed, paraffin-embedded) stained with NDC80 antibody AP22482PU-N at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Liver (formalin-fixed, paraffin-embedded) stained with NDC80 antibody AP22482PU-N at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Antibody staining (0.5 µg/ml) of Hela lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of NDC80 expression in HEK293T (A); SP2/0 (B); H9C2 (C) whole cell lysates.
ELISA: NDC80 Antibody
TA302826 staining (0.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of extracts from Jurkat cells, using KNTC2 antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HUVEC cells, using KNTC2 antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts of various cell lines, using NDC80 antibody.
Western blot analysis on Jurkat,A549 and HuvEc cell lysate using HEC1 Antibody
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302826 | NDC80 (C-term) antibody |
||||||
| Goat Polyclonal Antibody against KNTC2 | |||||||
 |
Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA315048 | NDC80 antibody |
||||||
| Rabbit polyclonal anti-KNTC2 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA332676 | NDC80 antibody |
||||||
| Rabbit anti-NDC80 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA347522 | NDC80 antibody |
||||||
| Rabbit Polyclonal Anti-HEC1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP22482PU-N | NDC80 (630-642) antibody |
||||||
 |
Goat | Aff - Purified | Hu | E, P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AP55047SU-N | NDC80 antibody |
||||||
 |
Rabbit | Serum | Hu | C, E, WB |
0.2 ml /
€430.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307565 | NDC80 |
||||
| Recombinant protein of human NDC80 homolog, kinetochore complex component (S. cerevisiae) (NDC80) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
