NDEL antibody
Principal name
NDEL antibody
Alternative names for NDEL antibody
EOPA, MITAP1, NUDEL, Nuclear distribution protein nudE-like 1
SwissProt ID
Q9GZM8 (Human)
Gene ID
81565 (NDEL1)
Ncbi ID
9606, NP_001020750.1, NP_001020750, NP_110435.1, NM_030808, NM_001025579, NP_110435, AAF97497
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)
Background of NDEL antibody
Nudel is important for normal cortical development. It is invovled in microtubule organization, nuclear translocation, and neuronal positioning in concert with various other factors (including Lis1, Pafah1b1, Pahfah1b2, dynein, dynorphin A and cdk5). Western blot analysis of mouse tissues shows abundant expression of Nudel in brain and testis, and much lower expression in heart, liver, kidney, and skeletal muscle. In fractionated rat brain, Nudel and Lis1 are both found in fractions enriched for postsynaptic density proteins. Immunostaining of embryonic day 18 mouse brain sections revealed staining of migrating neurons and thalamocortical axons of the intermediate zone of the developing cerebral cortex, as well as several other developing brain regions. The deduced protein contains 345 amino acids and has a calculated molecular mass of 38.4 kDa. It has a coiled coil motif (residues 19 to 201), followed by several potential phosphorylation sites for casein kinase II, protein kinase C or CDK5. Nudel shares about 50% identity with mouse and human NUDE proteins.
General readings
Yingling J, Youn YH, Darling D, Toyo-Oka K, Pramparo T, Hirotsune S, Wynshaw-Boris A.
Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric division.
Cell 2008 Feb 132 (3): 474-86.
PMID: 18267077
Western Blot analysis of NDEL1 expression in transfected 293T cell line (H00081565-T01) by NDEL1 MaxPab polyclonal antibody.Lane 1: NDEL1 transfected lysate(38.40 KDa).Lane 2: Non-transfected lysate.
NDEL1 polyclonal antibody ( Cat # PAB11614 ) ( 0.1 µg/ml ) staining of human cerebellum lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry of NDEL1 in Jurkat cells with NDEL1 polyclonal antibody ( Cat # PAB12877 ) at 2 µg/ml.
Western blot analysis of NDEL1 in Jurkat whole cell lysate with NDEL1 polyclonal antibody ( Cat # PAB12877 ) at 0.5, 1, and 2 µg/ml.
Immunocytochemical detection of 050603-1 Phospho-NUDEL on Methanol fixed HeLa cells with AM26510AF-N.
Western blot analysis of Phospho-NUDEL expression in HeLa cells using AM26510AF-N.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NDEL1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDEL1 (RC212323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDEL1.
HEK293T cells transfected with either RC212323 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NDEL1 antibody(TA503058), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-NDEL1 antibody(TA503058),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503061)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDEL1 (RC212323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDEL1.
Flow cytometric Analysis of Jurkat cells, using anti-NDEL1 antibody(TA503061),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC212323 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NDEL1 antibody(TA503061), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503204)
Anti-NDEL1 mouse monoclonal antibody (TA503204) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NDEL1(RC212323).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDEL1 (RC212323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDEL1.
HEK293T cells transfected with either RC212323 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NDEL1 antibody(TA503204), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDEL1 (RC212323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDEL1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NDEL1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-NDEL1 mouse monoclonal antibody (TA503313) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NDEL1(RC212323).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDEL1 (RC212323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDEL1.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503352)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503352)
Anti-NDEL1 mouse monoclonal antibody (TA503352) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NDEL1(RC212323).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDEL1 (RC212323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDEL1.
HEK293T cells transfected with either RC212323 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NDEL1 antibody(TA503352), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503353)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503353)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503353)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503353)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503353)
Anti-NDEL1 mouse monoclonal antibody (TA503353) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NDEL1(RC212323).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDEL1 (RC212323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDEL1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NDEL1 monoclonal antibody.
HEK293T cells transfected with either RC212323 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NDEL1 antibody(TA503353), and then analyzed by flow cytometry.
TA305793 (0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of Nudel in Jurkat whole cell lysate with Nudel antibody at (A) 0.5, (B) 1, or (C) 2 ug/mL.
Immunocytochemistry of Nudel in Jurkat cells with Nudel antibody at 2 ug/mL.
Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 ug/mL.
Western blot analysis of extracts of various cell lines, using NDEL1 antibody.
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (NDEL1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (5)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503058 | NDEL antibody |
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| NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI1G10 (formerly 1G10) | |||||||
 |
Mouse | IgG2b | OTI1G10 | Purified | Can, Hu, Mky, Rt | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA503061 | NDEL antibody |
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| NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI1C7 (formerly 1C7) | |||||||
 |
Mouse | IgG2b | OTI1C7 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA503204 | NDEL antibody |
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| NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI3B9 (formerly 3B9) | |||||||
 |
Mouse | IgG2a | OTI3B9 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA503352 | NDEL antibody |
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| NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI4E5 (formerly 4E5) | |||||||
 |
Mouse | IgG2a | OTI4E5 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA503353 | NDEL antibody |
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| NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI5E11 (formerly 5E11) | |||||||
 |
Mouse | IgG2a | OTI5E11 | Purified | Hu, Ms | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
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| TA305793 | NDEL (both isoforms) antibody |
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| Goat Anti-NDEL1 Antibody | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA306096 | NDEL antibody |
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| Rabbit Polyclonal Nudel Antibody | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms | E, ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA332908 | NDEL antibody |
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| Rabbit anti-NDEL1 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA350243 | NDEL antibody |
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| Rabbit Polyclonal Anti-NDEL1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA503254 | NDEL antibody |
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| NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI1G7 (formerly 1G7) | |||||||
 |
Mouse | IgG2a | OTI1G7 | Purified | Can, Hu, Ms, Rt | WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA503313 | NDEL antibody |
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| NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI5C2 (formerly 5C2) | |||||||
 |
Mouse | IgG2a | OTI5C2 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| AM26510AF-N | NDEL antibody |
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 |
Mouse | IgG1 | N219TP | Azide Free | Hu, Ms | ICC/IF, P, WB |
0.1 mg /
€460.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305974 | NDEL (transcript variant 2) |
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| Recombinant protein of human nudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NDEL1), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP312323 | NDEL (transcript variant 1) |
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| Recombinant protein of human nudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NDEL1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY410703 | NDEL1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY422448 | NDEL1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC205974L2V | Lenti ORF particles, NDEL1 (mGFP-tagged) - Human nudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NDEL1), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||