NDUFS8 antibody
Principal name
NDUFS8 antibody
Alternative names for NDUFS8 antibody
Complex I-23kD, TYKY subunit, CI-23kD, NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial, NADH-ubiquinone oxidoreductase 23 kDa subunit
Ncbi ID
9606, NM_002496, NP_002487, NP_002487.1, NP_659119.2, NM_144870
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of NDUFS8 antibody
- Kit Component:
- - KN310861G1, Ndufs8 gRNA vector 1 in pCas-Guide vector
- KN310861G2, Ndufs8 gRNA vector 2 in pCas-Guide vector
- KN310861D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Bourges, I., et al. Biochem. J. 383 (PT 3), 491-499 (2004)
Procaccio, V., et al. Neurology 62(10):1899-1901(2004)
Ugalde, C., et al. Hum. Mol. Genet. 13(6):659-667(2004)
Murray, J., et al. J. Biol. Chem. 278(39):37223-37230(2003)
Western Blot analysis of NDUFS8 expression in transfected 293T cell line (H00004728-T01) by NDUFS8 MaxPab polyclonal antibody.Lane 1: NDUFS8 transfected lysate(23.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NDUFS8 expression in transfected 293T cell line (H00004728-T01) by NDUFS8 MaxPab polyclonal antibody.Lane 1: NDUFS8 transfected lysate(23.70 KDa).Lane 2: Non-transfected lysate.
Human Jurkat; WB Suggested Anti-NDUFS8 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Jurkat Whole Cell; NDUFS8 antibody - C-terminal region (ARP61825_P050) in Human Jurkat cells using Western Blot
Human Fetal heart; WB Suggested Anti-NDUFS8 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Heart; NDUFS8 antibody - C-terminal region (ARP61826_P050) in Human Fetal heart cells using Western Blot
Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue reacted with NDUFS8 Antibody (Center) Cat.-No AP52845PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of NDUFS8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of NDUFS8 Antibody (Center) Cat.-No AP52845PU-N in Hela cell line lysates (35ug/lane). This demonstrates the NDUFS8 antibody detected the NDUFS8 protein (arrow).
Staining of Mouse Heart lysate using NDUFS8 Antibody Cat.-No AP32342PU-N at 0.1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample (50 ug of whole cell lysate). A: mouse brain. 12% SDS PAGE. TA307971 diluted at 1:1000.
NDUFS8 antibody detects NDUFS8 protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 12 % SDS-PAGE. NDUFS8 antibody (TA307971) dilution: 1:1000
Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. NDUFS8 antibody. TA307971 diluted at 1:1000.
NDUFS8 antibody detects NDUFS8 protein at on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. NDUFS8 antibody (TA307971) dilution: 1:500.
NDUFS8 antibody detects NDUFS8 protein at on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. NDUFS8 antibody (TA307971) dilution: 1:500.
NDUFS8 antibody detects NDUFS8 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. NDUFS8 antibody (TA307971) dilution: 1:500.
(0.1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-5: K562 cells, Hela cells, Jurkat cells, mouse heart tissue, Mouse spleen tissue, Primary antibody: (NDUFS8 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (NDUFS8 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (NDUFS8 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA307971 | NDUFS8 antibody |
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| Rabbit Polyclonal antibody to NDUFS8 (NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody! |
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| AP32342PU-N | NDUFS8 antibody |
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 |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt | E, WB |
0.1 mg /
€380.00
|
||
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
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| TA311237 | NDUFS8 antibody |
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| Goat Anti-NDUFS8 Antibody | |||||||
 |
Goat | Aff - Purified | Can, Hu, Ms, Rt | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA350220 | NDUFS8 antibody |
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| Rabbit Polyclonal Anti-NDUFS8 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| AP52845PU-N | NDUFS8 (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY400889 | NDUFS8 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR202305L2V | Lenti ORF particles, Ndufs8 (GFP-tagged) - Mouse NADH dehydrogenase (ubiquinone) Fe-S protein 8 (Ndufs8), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR202305L1V | Lenti ORF particles, Ndufs8 (Myc-DDK-tagged) - Mouse NADH dehydrogenase (ubiquinone) Fe-S protein 8 (Ndufs8), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||