NECAB1 antibody
Principal name
NECAB1 antibody
Alternative names for NECAB1 antibody
EFCBP1, N-terminal EF-hand calcium-binding protein 1
SwissProt ID
Q8N987 (Human)
Gene ID
64168 (NECAB1)
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F)
Background of NECAB1 antibody
- Kit Component:
- - KN209704G1, NECAB1 gRNA vector 1 in pCas-Guide vector
- KN209704G2, NECAB1 gRNA vector 2 in pCas-Guide vector
- KN209704D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of NECAB1 expression in transfected 293T cell line (H00064168-T02) by NECAB1 MaxPab polyclonal antibody.Lane 1: EFCBP1 transfected lysate(38.61 KDa).Lane 2: Non-transfected lysate.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NECAB1 (RC209704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NECAB1.
HEK293T cells transfected with either RC209704 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NECAB1 antibody(TA502520), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NECAB1 (RC209704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NECAB1.
HEK293T cells transfected with either RC209704 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NECAB1 antibody(TA502630), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-NECAB1 antibody(TA502630),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NECAB1 (RC209704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NECAB1.
HEK293T cells transfected with either RC209704 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NECAB1 antibody(TA502546), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-NECAB1 antibody(TA502546),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-NECAB1 antibody(TA502546),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502546 | NECAB1 antibody |
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| NECAB1 mouse monoclonal antibody, clone OTI4G8 (formerly 4G8) | |||||||
 |
Mouse | IgG2a | OTI4G8 | Purified | Hu | F, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA502520 | NECAB1 antibody |
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| NECAB1 mouse monoclonal antibody, clone OTI1H5 (formerly 1H5) | |||||||
 |
Mouse | IgG2a | OTI1H5 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA502630 | NECAB1 antibody |
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| NECAB1 mouse monoclonal antibody, clone OTI2H5 (formerly 2H5) | |||||||
 |
Mouse | IgG1 | OTI2H5 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309704 | NECAB1 |
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| Recombinant protein of human N-terminal EF-hand calcium binding protein 1 (NECAB1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||