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NEDD8 antibody

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Principal name

NEDD8 antibody

Alternative names for NEDD8 antibody

Neural precursor cell expressed developmentally down-regulated protein 8

SwissProt ID

P29595 (Mouse), Q15843 (Human), Q71UE8 (Rat)

Gene ID

4738 (NEDD8), 18002 (Nedd8), 25490

Ncbi ID

9606, NP_006147.1, NP_006147, NM_008683, NM_138878, NP_032709.1

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of NEDD8 antibody

Neural precursor cell-expressed developmentally downregulated protein 8 (NEDD8), also known as Rub1 (related to ubiquitin 1) in plants and yeast, is a member of the ubiquitin-like protein family. The covalent attachment of NEDD8 to target proteins, termed neddylation, is a reversible, multi-step process alogous to ubiquitition. NEDD8 is first synthesized in a precursor form with a carboxy-termil extension peptide that is removed by either the UCH-L3 or NEDP1/DEN1 hydrolase protein to yield a mature NEDD8 protein. Mature NEDD8 is then covalently linked to target proteins via the carboxy-termil glycine residue in a reaction catalyzed by the APP-BP1/Uba3 heterodimer complex and Ubc12 as the E1- and E2-like enzymes, respectively. An E3 ligase protein, Roc1/Rbx1, is also required for neddylation of the cullin proteins. Protein de-neddylation is catalyzed by a number of enzymes in the cell, including a "ubiquitin-specific" protease USP21, the NEDP1/DEN1 hydrolase and the COP9/siglosome (CSN). In contrast to the ubiquitin pathway, the NEDD8 modification system acts on only a few substrates and does not appear to target proteins for degradation. Neddylation of cullin proteins activates the SCF (Skp1-Cullin-F-box) E3 ubiquitin ligase complex by promoting complex formation and enhancing the recruitment of the E2-ubiquitin intermediate. While NEDD8 modification of VHL is not required for ubiquitition of HIF1-a, it is required for fibronectin matrix assembly. Mdm2-dependent neddylation of p53 inhibits its transcriptiol activity

General readings

Wu K., et al. (2002) J Biol Chem. 277(1): 516-27.

AP06450PU-N - NEDD8
Immunohistochemistry (IHC) analyzes of NEDD8 antibody (Cat.-No.: AP06450PU-N) in paraffin-embedded human lung carcinoma tissue.
AP06450PU-N - NEDD8
Western blot (WB) analysis of NEDD8 antibody (Cat.-No.: AP06450PU-N) in extracts from Jurkat cells.
PAB1737 - NEDD8
Formalin-fixed and paraffin-embedded human cancer tissue reacted with NEDD8 polyclonal antibody ( Cat # PAB1737 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
PAB1737 - NEDD8
Western blot analysis of NEDD8 polyclonal antibody ( Cat # PAB1737 ) in HeLa cell lysate. NEDD8 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
AR09126PU-L - NEDD8
AR09126PU-L - NEDD8
Recombinant human NEDD8, aa 1-76 (His-tagged): 15% SDS-PAGE (3 µg)
AP11227PU-N - NEDD8
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP11227PU-N - NEDD8
Western blot analysis of NEDD8 polyclonal antibody (Cat. #AP11227PU-N) in HeLa cell lysate. NEDD8 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
C0359-1 - NEDD8
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NEDD8 Antibody. The picture on the right is treated with the synthesized peptide.
C0359-1 - NEDD8
Western blot analysis of extracts from Jurkat cells, using NEDD8 Antibody. The lane on the right is treated with the synthesized peptide.
AP31875PU-N - NEDD8
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP31875PU-N - NEDD8
Western blot analysis of extracts from Jurkat cells, using NEDD8 Antibody. The lane on the right is treated with the synthesized peptide.
AM06718PU-N - NEDD8
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NEDD8 mouse mAb with DAB staining.
AM06718PU-N - NEDD8
Immunofluorescence analysis of Hela cells using NEDD8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
AM06718PU-N - NEDD8
Western blot analysis using NEDD8 mAb against human NEDD8 (AA: 1-81) recombinant protein. (Expected MW is 40 kDa)
AM06718PU-N - NEDD8
Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)
AM06718PU-N - NEDD8
Flow cytometric analysis of Hela cells using NEDD8 mouse mAb (green) and negative control (red).
AM06719PU-N - NEDD8
Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using NEDD8 antibody Cat.-No AM06719PU-N with DAB staining.
AM06719PU-N - NEDD8
Immunofluorescence analysis of Hela cells using NEDD8 antibody Cat.-No AM06719PU-N (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
AM06719PU-N - NEDD8
Immunofluorescence of HeLa cells using NEDD8 antibody Cat.-No AM06719PU-N (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin
AM06719PU-N - NEDD8
Western blot analysis using NEDD8 antibody Cat.-No AM06719PU-N against C6 (1) and Hela (2) cell lysate.
AM06719PU-N - NEDD8
Western blot analysis using NEDD8 antibody Cat.-No AM06719PU-N against human NEDD8 (AA: 1-81) recombinant protein (Expected MW is 40 kDa).
AM06719PU-N - NEDD8
Flow Cytometric analysis of Hela cells using NEDD8 antibody Cat.-No AM06719PU-N (green) and negative control (red).
CPA1793-100ul - NEDD8
Immunohistochemical analysis of NEDD8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA1793-100ul - NEDD8
Western blot analysis of NEDD8 expression in BJAB (A); K562 (B); HeLa (C) whole cell lysates.
TP760489 - NEDD8
TA314245 - NEDD8
Western blot analysis of extracts from Jurkat cells, using NEDD8 antibody.The lane on the right is treated with the synthesized peptide.
TA314245 - NEDD8
Western blot analysis of extracts from HuvEc cells (Lane 2), JK cells (Lane 3) and cos-7 cells (Lane 4), using NEDD8 Antibody. The lane on the left is treated with synthesized peptide.
TA314245 - NEDD8
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using NEDD8 antibody.The picture on the right is treated with the synthesized peptide.
TA326962 - NEDD8
Western blot analysis of extracts of various cell lines, using NEDD8 antibody.
TA326962 - NEDD8
Immunofluorescence analysis of HeLa cell using NEDD8 antibody. Blue: DAPI for nuclear staining.
TA347488 - NEDD8
Western blot analysis on Jurkat cell lysate using NEDD8 Antibody
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEDD8 antibody
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NEDD8 mouse mAb with DAB staining. Mouse IgG1 1A7 Purified Hu E, F, ICC/IF, P, WB
0.1 mg / €410.00
  OriGene Technologies GmbH
+4 additional images

Highly validated antibody!

NEDD8 antibody
Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using NEDD8 antibody Cat.-No AM06719PU-N with DAB staining. Mouse IgG1 5B8 Purified Hu E, F, ICC/IF, P, WB
0.1 mg / €410.00
  OriGene Technologies GmbH
+5 additional images

Highly validated antibody!

NEDD8 antibody
  Rabbit polyclonal anti-NEDD8 antibody  
Western blot analysis of extracts from Jurkat cells, using NEDD8 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+2 additional images

NEDD8 antibody
  NEDD8 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using NEDD8 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

NEDD8 antibody
  Rabbit Polyclonal Anti-NEDD8 Antibody  
Western blot analysis on Jurkat cell lysate using NEDD8 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

NEDD8 antibody
Immunohistochemistry (IHC) analyzes of NEDD8 antibody (Cat.-No.: AP06450PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

NEDD8 (N-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

NEDD8 (N-term) antibody
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µl / €425.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NEDD8 (N-term HIS tag)
  Purified recombinant protein of Human neural precursor cell expressed, developmentally down-regulated 8 (NEDD8), with N-terminal HIS tag, expressed in E.Coli, 50ug  
NEDD8 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Nedd8 (GFP-tagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nedd8 (GFP-tagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Nedd8 (Myc-DDK-tagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nedd8 (Myc-DDK-tagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Nedd8 (GFP-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated 8 (Nedd8), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nedd8 (GFP-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated 8 (Nedd8), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Nedd8 (Myc-DDK-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated 8 (Nedd8), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nedd8 (Myc-DDK-tagged ORF) - Rat neural precursor cell expressed, developmentally down-regulated 8 (Nedd8), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

13 Item(s)

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13 products - FILTER YOUR RESULTS!

Available Filters

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  1. Mouse Lenti ORF Particle (2)
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  3. Protein | Growth Factor (1)
  4. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (6)
  2. Monoclonal (2)
Target
  1. NEDD8 (9)
Property
  1. N-term (2)
Reactivity
  1. Human (Hu) (8)
  2. Mouse (Ms) (4)
  3. Rat (Rt) (4)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Paraffin Sections (P) (6)
  3. Enzyme Immunoassay (E) (4)
  4. Flow cytometry (F) (2)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
Presentation
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  2. Aff - Purified (2)
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  2. Mouse (2)
Images
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  3. 5+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (8)
  2. Paraffin Sections (P) (5)
  3. Flow cytometry (F) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  5. Enzyme Immunoassay (E) (1)
  6. Immunofluorescence (IF) (1)
  7. IHC (1)
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