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NEDD9 antibody

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Principal name

NEDD9 antibody

Alternative names for NEDD9 antibody

CASL, CASS2, hEF1, Enhancer of filamentation 1,Neural precursor cell expressed developmentally down-regulated protein 9

SwissProt ID

Q14511 (Human)

Gene ID

4739 (NEDD9)

Ncbi ID

9606, NP_006394, NP_001135865.1, NM_006403, NP_001135865, NM_182966, NM_001142393

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C)

Background of NEDD9 antibody

HEF1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases; two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.

General readings

Kim M, Gans JD, Nogueira C, Wang A, Paik JH, Feng B, Brennan C, Hahn WC, Cordon-Cardo C, Wagner SN, Flotte TJ, Duncan LM, Granter SR, Chin L.
Comparative oncogenomics identifies NEDD9 as a melanoma metastasis gene.
Cell. 2006 Jun 30;125(7):1269-81.
PMID: 16814714

PAB7323 - NEDD9
NEDD9 polyclonal antibody ( Cat # PAB7323 ) ( 0.1 µg/ml ) staining of human kidney lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
H00004739-B01P - NEDD9
Immunofluorescence of purified MaxPab antibody to NEDD9 on HeLa cell. [antibody concentration 10 ug/ml]
H00004739-B01P - NEDD9
Western Blot analysis of NEDD9 expression in transfected 293T cell line (H00004739-T01) by NEDD9 MaxPab polyclonal antibody.Lane 1: NEDD9 transfected lysate(91.74 KDa).Lane 2: Non-transfected lysate.
H00004739-B01P - NEDD9
NEDD9 MaxPab polyclonal antibody. Western Blot analysis of NEDD9 expression in 293.
H00004739-D01P - NEDD9
NEDD9 MaxPab rabbit polyclonal antibody. Western Blot analysis of NEDD9 expression in mouse brain.
H00004739-D01P - NEDD9
Western Blot analysis of NEDD9 expression in transfected 293T cell line (H00004739-T01) by NEDD9 MaxPab polyclonal antibody.Lane 1: NEDD9 transfected lysate(92.90 KDa).Lane 2: Non-transfected lysate.
ARP51210_T100 - NEDD9
Human kidney; NEDD9 antibody - middle region (ARP51210_T100) in Human kidney cells using Immunohistochemistry
ARP51210_T100 - NEDD9
Human 721_B; WB Suggested Anti-NEDD9 Antibody Titration: 5.0ug/ml. Positive Control: 721_B cell lysate. NEDD9 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; NEDD9 antibody - middle region (ARP51210_T100) in Human 721_B cells using Western Blot
MAB2091 - NEDD9
Immunofluorescence microscopyusing NEDD9 monoclonal antibody, clone 14A11 ( Cat # MAB2091 ) shows detectionof NEDD9 localized at the centrosome ( bright dots ) and focal adhesion sites.The antibody was used at a 1:100 dilution.Personal Communication. Elena Pugacheva, FoxChase Cancer Center, Philadelphia, PA.
MAB2091 - NEDD9
Western blotting using NEDD9 monoclonal antibody, clone 14A11 ( Cat # MAB2091 ) shows detection of NEDD9 present in MCF-7 cells induced to express NEDD9 by tetracycline removal ( right lane ).See Pugacheva et al for details.
ARP51211_P050 - NEDD9
Human 293T; WB Suggested Anti-NEDD9 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: 293T cell lysate; NEDD9 antibody - middle region (ARP51211_P050) in Human 293T cells using Western Blot
TP307200 - NEDD9
TP327437 - NEDD9
TA303260 - NEDD9
TA303260 (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA319568 - NEDD9
WB using monoclonal anti-HEF1 antibody antibody shows detection of a 115 kDa band corresponding to HEF1 in MCF7 lysate (arrowhead). Approximately 35 ug of lysate was loaded for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,000 dilution of anti-HEF1 antibody. Detection occurred using a 1:5,000 dilution of IRDye®800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers (indicated at left).
TA319568 - NEDD9
WB using monoclonal anti-HEF1 antibody (clone 2G9) shows detection of endogenous HEF1 present in various cell lines. Panel A shows detection using a 15 min exposure. Panel B is the same blot exposed for 2 min. The doublet represents p105 and p115 staining. The lower MW band represents p55. 3Y1 cells are derived from rat fibroblast cells. Mouse 3T3 cells are also reactive (not shown). To date no staining has been noted on CHO cells.
TA319568 - NEDD9
Immunofluorescence microscopy using Monoclonal anti-HEF1 antibody (clone 2G9) shows detection of HEF1 localized at focal adhesion sites. The antibody was used at a 1:500 dilution with a 3-sec exposure time. Personal Communication. Elena Pugacheva, Fox Chase Cancer Center, Philadelphia, PA.
TA319569 - NEDD9
Western blotting using Monoclonal anti-HEF1 antibody (clone 14A11) shows detection of HEF1 present in MCF-7 cells induced to express HEF1 by tetracycline removal (right lane). See Pugacheva et al for details.
TA319569 - NEDD9
Immunofluorescence microscopy using Monoclonal anti-HEF1 antibody (clone 14A11) shows detection of HEF1 localized at the centrosome (bright dots) and focal adhesion sites. The antibody was used at a 1:100 dilution with a 1-min exposure time. Personal Communication. Elena Pugacheva, Fox Chase Cancer Center, Philadelphia, PA.
TA339893 - NEDD9
WB Suggested Anti-NEDD9 Antibody Titration: 5.0 ug/ml; Positive Control: 721_B cell lysate NEDD9 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
TA339893 - NEDD9
Human kidney
TA339894 - NEDD9
WB Suggested Anti-NEDD9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: 293T cell lysate
TA350044 - NEDD9
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Raji cells, Primary antibody: (NEDD9 Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds

8 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEDD9 antibody
  Goat Polyclonal Antibody against NEDD9  
TA303260 (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

NEDD9 antibody
  Mouse monoclonal anti-HEF1 antibody  
WB using monoclonal anti-HEF1 antibody antibody shows detection of a 115 kDa band corresponding to HEF1 in MCF7 lysate (arrowhead). Approximately 35 ug of lysate was loaded for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,000 dilution of anti-HEF1 antibody. Detection occurred using a 1:5,000 dilution of IRDye®800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). Mouse 2G9 Purified Hu E, ICC/IF, IP, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.
+2 additional images

NEDD9 antibody
  Mouse monoclonal anti-HEF1 antibody  
Western blotting using Monoclonal anti-HEF1 antibody (clone 14A11) shows detection of HEF1 present in MCF-7 cells induced to express HEF1 by tetracycline removal (right lane). See Pugacheva et al for details. Mouse 14A11 Purified Hu E, ICC/IF, IP, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.
+1 additional image

NEDD9 antibody
  Rabbit Polyclonal Anti-NEDD9 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Raji cells, Primary antibody: (NEDD9 Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds Rabbit IgG Purified Hu E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

NEDD9 pSer369 antibody
NEDD9 Rabbit Serum Hu E, ICC/IF, WB
0.2 ml / €430.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NEDD9 (transcript variant 1)
  Recombinant protein of human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1  
NEDD9 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

NEDD9 (transcript variant 3)
  Recombinant protein of human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 3  
NEDD9 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

NEDD9 overexpression lysate
NEDD9 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

8 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (5)
  3. Protein | Growth Factor (2)
Clonality
  1. Polyclonal (3)
  2. Monoclonal (2)
Target
  1. NEDD9 (7)
Property
  1. pSer369 (1)
Reactivity
  1. Human (Hu) (5)
  2. Canine (Can) (1)
  3. Mouse (Ms) (1)
  4. Rat (Rt) (1)
Applications
  1. Enzyme Immunoassay (E) (5)
  2. Western blot / Immunoblot (WB) (5)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
  4. Immunoprecipitation (IP) (2)
Presentation
  1. Purified (3)
  2. Aff - Purified (1)
  3. Serum (1)
Host
  1. Mouse (2)
  2. Rabbit (2)
  3. Goat (1)
Images
  1. 1 image (6)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Immunofluorescence (IF) (2)
Species (Protein)
  1. Human (2)
Source
  1. HEK293 cells (2)
Manufacturer
  1. OriGene Technologies (8)
Service
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