NEDL2 antibody
Principal name
NEDL2 antibody
Alternative names for NEDL2 antibody
HECT, HECW2, KIAA1301, NEDD4-like E3 ubiquitin-protein ligase 2
SwissProt ID
Q9P2P5 (Human)
Gene ID
57520 (HECW2)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of NEDL2 antibody
- Kit Component:
- - KN216544G1, HECW2 gRNA vector 1 in pCas-Guide vector
- KN216544G2, HECW2 gRNA vector 2 in pCas-Guide vector
- KN216544D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Miyazaki, K., et al. Biochem. Biophys. Res. Commun. 308(1):106-113(2003)
Detection limit for recombinant GST tagged HECW2 is approximately 0.3ng/ml as a capture antibody.
Western blot analysis of HECW2 Antibody (Center) Cat.-No AP52027PU-N in mouse brain tissue lysates (35ug/lane). This demonstrates the HECW2 antibody detected the HECW2 protein (arrow).
Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of HECW2 expression in A549 (A); NIH3T3 (B) whole cell lysates.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP52027PU-N | NEDL2 (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP316544 | NEDL2 |
||||
| Recombinant protein of human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY412317 | HECW2 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
