NEIL2 antibody
Principal name
NEIL2 antibody
Alternative names for NEIL2 antibody
Nei-like 2, NEH2, Endonuclease VIII-like 2
Gene ID
252969 (NEIL2)
Ncbi ID
9606, NP_001129218, NP_001129220.1, NP_659480.1, NM_001135748, NP_659480, NP_001129218.1, NM_145043, NM_001135747
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP)
Background of NEIL2 antibody
- Kit Component:
- - KN200913G1, NEIL2 gRNA vector 1 in pCas-Guide vector
- KN200913G2, NEIL2 gRNA vector 2 in pCas-Guide vector
- KN200913D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press
Grin, I.R., et al. Biochem. Biophys. Res. Commun. 394(1):100-105(2010)
Kinslow, C.J., et al. Genes Chromosomes Cancer 47(11):923-932(2008)
Zhai, X., et al. Clin. Cancer Res. 14(13):4345-4352(2008)
Western Blot analysis of NEIL2 expression in transfected 293T cell line by NEIL2 monoclonal antibody (M01), clone 1B7.Lane 1: NEIL2 transfected lysate(36.8 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of NEIL2 over-expressed 293 cell line, cotransfected with NEIL2 Validated Chimera RNAi ( Cat # H00252969-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NEIL2 monoclonal antibody (M01), clone 1B7 (Cat # H00252969-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Detection limit for recombinant GST tagged NEIL2 is approximately 1ng/ml as a capture antibody.
Western Blot analysis of NEIL2 expression in transfected 293T cell line (H00252969-T01) by NEIL2 MaxPab polyclonal antibody.Lane 1: NEIL2 transfected lysate(36.52 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NEIL2 expression in transfected 293T cell line (H00252969-T02) by NEIL2 MaxPab polyclonal antibody.Lane 1: NEIL2 transfected lysate(36.80 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NEIL2 expression in transfected 293T cell line (H00252969-T02) by NEIL2 MaxPab polyclonal antibody.Lane 1: NEIL2 transfected lysate(36.80 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of NEIL2 transfected lysate using anti-NEIL2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NEIL2 MaxPab mouse polyclonal antibody (B01) (H00252969-B01).
Western blot analysis of NEIL2 Antibody (N-term) Cat.-No AP52850PU-N in HepG2 cell line lysates (35ug/lane). This demonstrates the NEIL2 antibody detected the NEIL2 protein (arrow).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP52850PU-N | NEIL2 (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300913 | NEIL2 (transcript variant 1) |
||||
| Recombinant protein of human nei like 2 (E. coli) (NEIL2), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP327701 | NEIL2 (transcript variant 2) |
||||
| Recombinant protein of human nei like 2 (E. coli) (NEIL2), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY427694 | NEIL2 overexpression lysate |
|
 |
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |