NEK11 antibody
Principal name
NEK11 antibody
Alternative names for NEK11 antibody
NIMA - related kinase 11, Serine/threonine-protein kinase Nek11
SwissProt ID
Q8NG66 (Human)
Gene ID
79858 (NEK11)
Ncbi ID
9606, NP_079076, NP_001139475.1, NP_079076.2, NM_024800, NM_145910, NM_001146003
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F)
Background of NEK11 antibody
- Kit Component:
- - KN221953G1, NEK11 gRNA vector 1 in pCas-Guide vector
- KN221953G2, NEK11 gRNA vector 2 in pCas-Guide vector
- KN221953D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 3ug/ml]
Immunofluorescence of monoclonal antibody to NEK11 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of NEK11 expression in transfected 293T cell line by NEK11 monoclonal antibody (M01), clone 4E1-1F4.Lane 1: NEK11 transfected lysate(55.5 KDa).Lane 2: Non-transfected lysate.
NEK11 monoclonal antibody (M01), clone 4E1-1F4. Western Blot analysis of NEK11 expression in A-431 ( Cat # L015V1 ).
NEK11 monoclonal antibody (M01), clone 4E1-1F4 Western Blot analysis of NEK11 expression in HL-60 ( Cat # L014V1 ).
Western blot analysis of NEK11 polyclonal antibody ( Cat # PAB3280 ) in mouse brain tissue lysate. NEK11 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot analysis of NEK11 expression in transfected 293T cell line (H00079858-T01) by NEK11 MaxPab polyclonal antibody.Lane 1: NEK11 transfected lysate(53.02 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NEK11 expression in transfected 293T cell line (H00079858-T02) by NEK11 MaxPab polyclonal antibody.Lane 1: NEK11 transfected lysate(55.50 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of anti-NEK11L Pab in mouse brain tissue lysate. NEK11L (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Hum. Adult Placenta; Host:Rabbit. Target Name:NEK11. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; NEK11 antibody - middle region (ARP48920_P050) in Hum. Adult Placenta cells using Western Blot
Hum. Fetal Heart; Host:Rabbit. Target Name:NEK11. Sample Tissue:Human Fetal Heart. Antibody Dilution: 1.0ug/ml.; NEK11 antibody - middle region (ARP48920_P050) in Hum. Fetal Heart cells using Western Blot
Hum. Fetal Lung; Host:Rabbit. Target Name:NEK11. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; NEK11 antibody - middle region (ARP48920_P050) in Hum. Fetal Lung cells using Western Blot
Hum. Fetal Muscle; Host:Rabbit. Target Name:NEK11. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; NEK11 antibody - middle region (ARP48920_P050) in Hum. Fetal Muscle cells using Western Blot
Human 293T; WB Suggested Anti-NEK11 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate. NEK11 is supported by BioGPS gene expression data to be expressed in HEK293T.; NEK11 antibody - middle region (ARP48920_P050) in Human 293T cells using Western Blot
Anti-NEK11 mouse monoclonal antibody (TA501583) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK11(RC221953).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK11 (RC221953, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK11.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK11 (RC221953, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK11.
HEK293T cells transfected with either RC221953 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NEK11 antibody(TA501608), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NEK11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501765)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NEK11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501765)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NEK11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501765)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NEK11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501765)
Anti-NEK11 mouse monoclonal antibody (TA501765) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK11(RC221953).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK11 (RC221953, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK11.
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A549 cells, 231 cells, Primary antibody: (NEK11 Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
Host: Rabbit; Target Name: NEK11; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: NEK11; Sample Tissue: Human Fetal Muscle; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: NEK11; Sample Tissue: Human Fetal Heart; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: NEK11; Sample Tissue: Human Fetal Lung; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-NEK11 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate; NEK11 is supported by BioGPS gene expression data to be expressed in HEK293T
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA501765 | NEK11 antibody |
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| NEK11 mouse monoclonal antibody, clone OTI5D9 (formerly 5D9) | |||||||
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Mouse | IgG1 | OTI5D9 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody!
|
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| TA349609 | NEK11 antibody |
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| Rabbit Polyclonal Anti-NEK11 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA501583 | NEK11 antibody |
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| anti-NEK11 mouse monoclonal antibody, clone OTI4H5 (formerly 4H5) | |||||||
 |
Mouse | IgG1 | OTI4H5 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA501608 | NEK11 antibody |
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| anti-NEK11 mouse monoclonal antibody, clone OTI5E9 (formerly 5E9) | |||||||
 |
Mouse | IgG1 | OTI5E9 | Purified | Hu | F, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| AP15018PU-N | NEK11 (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP321953 | NEK11 (transcript variant 1) |
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| Recombinant protein of human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
