NEK2 antibody
Principal name
NEK2 antibody
Alternative names for NEK2 antibody
NLK1, NimA-related protein kinase 2, HSPK 21, Serine/threonine-protein kinase Nek2
Gene ID
4751 (NEK2)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of NEK2 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Immunoperoxidase of monoclonal antibody to NEK2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1 ug/ml]
Western Blot analysis of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody (M01), clone 2F6.Lane 1: NEK2 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged NEK2 is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to NEK2 on HeLa cell. [antibody concentration 30 ug/ml]
NEK2 monoclonal antibody (M02), clone 2F9 Western Blot analysis of NEK2 expression in PC-12 ( Cat # L012V1 ).
Detection limit for recombinant GST tagged NEK2 is approximately 3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NEK2 is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NEK2 is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to NEK2 on HeLa cell. [antibody concentration 25 ug/ml]
NEK2 monoclonal antibody (M11), clone 3B7. Western Blot analysis of NEK2 expression in NIH/3T3 ( Cat # L018V1 ).
NEK2 monoclonal antibody (M11), clone 3B7 Western Blot analysis of NEK2 expression in MCF-7 ( Cat # L046V1 ).
NEK2 monoclonal antibody (M11), clone 3B7. Western Blot analysis of NEK2 expression in PC-12 ( Cat # L012V1 ).
NEK2 monoclonal antibody (M11), clone 3B7. Western Blot analysis of NEK2 expression in Raw 264.7 ( Cat # L024V1 ).
Detection limit for recombinant GST tagged NEK2 is approximately 3ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to NEK2 on HeLa cell. [antibody concentration 10 ug/ml]
NEK2 MaxPab polyclonal antibody. Western Blot analysis of NEK2 expression in rat brain.
Western Blot analysis of NEK2 expression in transfected 293T cell line (H00004751-T01) by NEK2 MaxPab polyclonal antibody.Lane 1: NEK2 transfected lysate(48.95 KDa).Lane 2: Non-transfected lysate.
Prostate: Formalin-Fixed Paraffin-Embedded (FFPE)
The NEK2 polyclonal antibody ( Cat # PAB3283 ) is used in Western blot to detect NEK2 in HeLa cell lysate.
Western blot analysis of NEK2 polyclonal antibody ( Cat # PAB3284 ) in mouse lung tissue lysate. NEK2 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunofluorescence of purified MaxPab antibody to NEK2 on HeLa cell. [antibody concentration 10 ug/ml]
NEK2 MaxPab polyclonal antibody. Western Blot analysis of NEK2 expression in rat brain.
Western Blot analysis of NEK2 expression in transfected 293T cell line (H00004751-T01) by NEK2 MaxPab polyclonal antibody.Lane 1: NEK2 transfected lysate(48.95 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NEK2 expression in transfected 293T cell line (H00004751-T02) by NEK2 MaxPab polyclonal antibody.Lane 1: NEK2 transfected lysate(51.80 KDa).Lane 2: Non-transfected lysate.
NEK2 MaxPab rabbit polyclonal antibody. Western Blot analysis of NEK2 expression in HeLa.
The anti-NEK2 Pab is used in Western blot to detect NEK2 in HeLa cell lysate.
Western blot analysis of anti-NEK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). NEK2(arrow) was detected using the purified Pab.
Western blot analysis is shown using NEK2 polyclonal antibody ( Cat # PAB10072 ) to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate ( arrowhead ). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein. The blot was incubated with a 1 : 500 dilution of the antibody at room temperature followed by detection using standard techniques.Personal communication Steven Pelech, Kinexus Inc.
Western blot showing NEK2 polyclonal antibody ( Cat # PAB12347 ) on recombinant His6 protein.
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody clone 2F6.
Detection limit for recombinant GST tagged NEK2 is approximately 0.3ng/ml as a capture antibody.
Western blot analysis is shown using Affinity Purified anti-NEK2 antibody to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed monoclonal antibody against NEK-2 (clone 8A6). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Western blot analysis of extracts of various cell lines, using NEK2 antibody.
Immunohistochemistry of paraffin-embedded human kidney cancer using NEK2 antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using NEK2 antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of U2OS cell using NEK2 antibody. Blue: DAPI for nuclear staining.
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Lovo cells, Primary antibody: (NEK2 Antibody) at dilution 1/727, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
Host: Rabbit; Target Name: NEK2; Sample Tissue: Thyroid Tumor lysates; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: NEK2; Sample Tissue: HT1080 lysates; Antibody Dilution: 1.0 ug/ml
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- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA332633 | NEK2 antibody |
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| Rabbit anti-NEK2 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA319262 | NEK2 antibody |
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| Rabbit polyclonal anti-NEK2 antibody | |||||||
 |
Rabbit | Aff - Purified | Hu | E |
0.1 mg /
€515.00
|
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| OriGene Technologies, Inc. | |||||||
| TA349610 | NEK2 antibody |
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| Rabbit Polyclonal Anti-NEK2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM31131PU-N | NEK2 (331-445) antibody |
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Mouse | IgG2a | 2F6 | Purified | Hu | E, IP, P, WB |
50 µg /
€475.00
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| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP07250PU-N | NEK2 antibody |
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 |
Rabbit | Aff - Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| AP15021PU-N | NEK2 antibody |
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Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| AP15022PU-N | NEK2 antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
