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NEK4 antibody

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Principal name

NEK4 antibody

Alternative names for NEK4 antibody

NimA-related protein kinase 4, STK2, Serine/threonine-protein kinase Nek4, Serine/threonine-protein kinase 2, Serine/threonine-protein kinase NRK2

SwissProt ID

A5YM70 (Human), P51957 (Human), Q9Z1J2 (Mouse)

Gene ID

6787 (NEK4), 23955 (Nek4)

Ncbi ID

9606, NP_003148.2, NP_001180462.1, NM_011849, NM_003157

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NEK4 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

H00006787-M01 - NEK4
Detection limit for recombinant GST tagged NEK4 is approximately 0.3ng/ml as a capture antibody.
H00006787-M02 - NEK4
Detection limit for recombinant GST tagged NEK4 is approximately 0.3ng/ml as a capture antibody.
PAB3286 - NEK4
Formalin-fixed and paraffin-embedded human cancer tissue reacted with NEK4 polyclonal antibody ( Cat # PAB3286 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
PAB3286 - NEK4
Western blot analysis of NEK4 polyclonal antibody ( Cat # PAB3286 ) in mouse brain tissue lysate. NEK4 ( Arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
AP15024PU-N - NEK4
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
AP15024PU-N - NEK4
Western blot analysis of anti-NEK4 Pab in Y79 cell line lysates (35ug/lane). NEK4 (arrow) was detected using the purified Pab.
TA308520 - NEK4
Sample (30 ug of whole cell lysate). A: A431 . B: H1299. 7.5% SDS PAGE. TA308520 diluted at 1:1000.
TA308520 - NEK4
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using NEK4(TA308520) antibody at 1:100 dilution.
TA308520 - NEK4
NEK4 antibody [N2C1], Internal detects NEK4 protein at nucleus by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 15 min. Green: NEK4 protein stained by NEK4 antibody [N2C1], Internal (TA308520) diluted at 1:500. Bl
TA350920 - NEK4
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (NEK4 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
TA350920 - NEK4
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (NEK4 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEK4 antibody
  Rabbit polyclonal antibody to NEK4 (NIMA (never in mitosis gene a)-related kinase 4)  
Sample (30 ug of whole cell lysate). A: A431 . B: H1299. 7.5% SDS PAGE. TA308520 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+2 additional images

NEK4 antibody
  Rabbit Polyclonal Anti-NEK4 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (NEK4 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

NEK4 (C-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Nek4 (GFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nek4 (GFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), 200 uL, >10^7 TU/mL  
200 µl / €1,590.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Nek4 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), 200 uL, >10^7 TU/mL
Lenti ORF particles, Nek4 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), 200 uL, >10^7 TU/mL  
200 µl / €1,430.00
  OriGene Technologies, Inc.
 

5 Item(s)

per page

Available Filters

Product Type
  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (3)
Clonality
  1. Polyclonal (3)
Target
  1. NEK4 (3)
Property
  1. C-term (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (2)
Applications
  1. Paraffin Sections (P) (3)
  2. Western blot / Immunoblot (WB) (2)
  3. Enzyme Immunoassay (E) (1)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Purified (2)
  2. Aff - Purified (1)
Host
  1. Rabbit (3)
Images
  1. 1 image (3)
  2. 3+ images (1)
Images: Applications
  1. IHC (2)
  2. Western blot / Immunoblot (WB) (2)
  3. Immunofluorescence (IF) (1)
  4. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (5)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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