NEK7 antibody
Principal name
NEK7 antibody
Alternative names for NEK7 antibody
NIMA related kinase 7, Serine/threonine-protein kinase Nek7
SwissProt ID
Q8TDX7 (Human)
Gene ID
140609 (NEK7)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of NEK7 antibody
- Kit Component:
- - KN211857G1, NEK7 gRNA vector 1 in pCas-Guide vector
- KN211857G2, NEK7 gRNA vector 2 in pCas-Guide vector
- KN211857D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Olson JE, Wang X, Pankratz VS, Fredericksen ZS, Vachon CM, Vierkant RA, Cerhan JR, Couch FJ, Centrosome-related genes, genetic variation, and risk of breast cancer.
Breast Cancer Res Treat. 2011 Jan;125(1):221-8.
PMID: 20508983
Formalin-fixed and paraffin-embedded human cancer tissue reacted with NEK7 polyclonal antibody ( Cat # PAB3288 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Western Blot analysis of NEK7 expression in transfected 293T cell line (H00140609-T01) by NEK7 MaxPab polyclonal antibody.Lane 1: NEK7 transfected lysate(33.22 KDa).Lane 2: Non-transfected lysate.
NEK7 MaxPab polyclonal antibody. Western Blot analysis of NEK7 expression in HeLa.
Human HepG2; WB Suggested Anti-NEK7 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; NEK7 antibody - N-terminal region (ARP48999_P050) in Human HepG2 cells using Western Blot
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of NEK7 (arrow) using NEK7 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK7 gene (Lane 2) (Origene Technologies).
AP22436PU-N NEK7 antibody staining of HeLa lysate at 0.05 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample(30 ug of whole cell lysate). A:HeLa S3. 12% SDS PAGE. TA308429 diluted at 1:500
Immunohistochemical analysis of paraffin-embedded Tw2.6 xenograft, using NEK7(TA308429) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NEK7(TA308429) antibody at 1:200 dilution.
TA311568 (0.1 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA311568 (0.3 ug/ml) staining of Mouse Lung (A), Mouse Testis (B), Rat Lung (C) and Rat Testis (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-NEK7 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK7 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK7 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK7 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK7 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK7 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP22436PU-N | NEK7 antibody |
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt | E, P, WB |
0.1 mg /
€380.00
|
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
OriGene recommends this product for Western blotting. |
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| TA308429 | NEK7 antibody |
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| Rabbit Polyclonal antibody to NEK7 (NIMA (never in mitosis gene a)-related kinase 7) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA311568 | NEK7 (274-286) antibody |
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| Goat Anti-NEK7 (aa 274-286) Antibody | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA340996 | NEK7 (N-term) antibody |
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| Rabbit Polyclonal Anti-NEK7 Antibody (N-Terminus) | |||||||
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Rabbit | IgG | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | P |
50 µg /
€425.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA340997 | NEK7 (Kinase Dom.) antibody |
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| Rabbit Polyclonal Anti-NEK7 Antibody (Kinase Domain) | |||||||
|
Rabbit | Aff - Purified | Hu | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| AP15026PU-N | NEK7 (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP311857 | NEK7 |
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| Recombinant protein of human NIMA (never in mitosis gene a)-related kinase 7 (NEK7) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||