NEK9 antibody
Principal name
NEK9 antibody
Alternative names for NEK9 antibody
Never in mitosis A-related kinase 9, Nercc1 kinase, KIAA1995, NEK8, NERCC, NIMA-related kinase 8
Gene ID
91754 (NEK9)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)
Background of NEK9 antibody
Nek8 (also designated never in mitosis A (NimA)-related kinase 8, Jck and NEK12A) is a mitotic regulator that plays a significant role in maintaining renal tubular integrity. Nek8 transcript is abundant in thyroid, adrenal gland and skin, with overexpression in breast tumors and infiltrating ductal carcinomas, and moderate levels in mucinous adenocarcinoma. Nek8 mutations correlate to juvenile autosomal recessive polycystic kidney disease in humans. Nek9 (never in mitosis A (NimA)-related kinase 9, Nercc1 kinase) regulates G1/S transition and S phase progression by influencing spindle dynamics and chromosome separation. Nek9 phosphorylates different histones (Histone H3 on serine and threonine), myelin basic protein, β-casein (serine) and BICD2. Nek9 interacts with Ran GTPase, Nek6, Nek7, BICD2, SSRP1 and SUPT16H/ FACT complex. Nek9 transcript is abundant in heart, liver, kidney and testis. Nek family cell-cycle protein kinases in humans have at least 11 members that share an amino-terminal catalytic domain related to NIMA, an Aspergillus nidulans kinase that plays on several aspects of mitosis, with divergent carboxyl-terminal tails of varying length.
Immunofluorescence of monoclonal antibody to NEK9 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of NEK9 expression in transfected 293T cell line by NEK9 monoclonal antibody (M01), clone 1F6.Lane 1: NEK9 transfected lysate(107.2 KDa).Lane 2: Non-transfected lysate.
NEK9 monoclonal antibody (M01), clone 1F6 Western Blot analysis of NEK9 expression in HeLa ( Cat # L013V1 ).
NEK9 monoclonal antibody (M01), clone 1F6. Western Blot analysis of NEK9 expression in NIH/3T3(Cat # L018V1 ).
NEK9 monoclonal antibody (M01), clone 1F6. Western Blot analysis of NEK9 expression in Raw 264.7(Cat # L024V1 ).
NEK9 monoclonal antibody (M01), clone 1F6. Western Blot analysis of NEK9 expression in PC-12(Cat # L012V1 ).
Detection limit for recombinant GST tagged NEK9 is approximately 0.03ng/ml as a capture antibody.
Brain, cortex
Formalin-fixed and paraffin-embedded human cancer tissue reacted with NEK9 polyclonal antibody ( Cat # PAB3289 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
The NEK9 polyclonal antibody ( Cat # PAB3289 ) is used in Western blot to detect NEK9 in 293 cell lysate ( Lane 1 ) and mouse heart tissue lysate ( Lane 2 ) .
Western Blot analysis of NEK9 expression in transfected 293T cell line (H00091754-T01) by NEK9 MaxPab polyclonal antibody.Lane 1: NEK9 transfected lysate(107.69 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NEK9 expression in transfected 293T cell line (H00091754-T01) by NEK9 MaxPab polyclonal antibody.Lane 1: NEK9 transfected lysate(107.69 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of NEK9 transfected lysate using anti-NEK9 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NEK9 MaxPab mouse polyclonal antibody (B01) (H00091754-B01).
Human THP-1; WB Suggested Anti-NEK9 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: THP-1 cell lysate; NEK9 antibody - middle region (ARP48976_P050) in Human THP-1 cells using Western Blot
Western blot analysis of Nek9 antibody (AP20364PU-N) in extracts from A549 cells.
Anti-NEK9 mouse monoclonal antibody (TA503326) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK9(RC211326).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK9 (RC211326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK9.
HEK293T cells transfected with either RC211326 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NEK9 antibody(TA503326), and then analyzed by flow cytometry.
Anti-NEK9 mouse monoclonal antibody (TA503367) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK9(RC211326).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK9 (RC211326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK9.
HEK293T cells transfected with either RC211326 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NEK9 antibody(TA503367), and then analyzed by flow cytometry.
Western blot analysis of extracts from A549 cells, using NEK9 (Ab-210) antibodyThe lane on the right is treated with the synthesized peptide.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-NEK9 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Formalin-fixed paraffin-embedded human brain cortex tissue was stained with Poly6241 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Immunohistochemistry-Paraffin: NEK9 Antibody [TA337193] - IHC analysis of Brain using TA337193 at 4 ug/ ml.
Anti-NEK9 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK9 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (NEK9 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (NEK9 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
WB Suggested Anti-NEK9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: THP-1 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA312627 | NEK9 antibody |
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| Rabbit polyclonal NEK9 (Ab-210) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA328043 | NEK9 antibody |
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| Rabbit Polyclonal anti-NEK9 Antibody | |||||||
 |
Rabbit | IgG | Poly6241 | Purified | Hu | IP, P, WB |
0.2 ml /
€275.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA340998 | NEK9 (Kinase Dom.) antibody |
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| Rabbit Polyclonal Anti-NEK9 Antibody (Kinase Domain) | |||||||
 |
Rabbit | Aff - Purified | Hu, Ms | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341012 | NEK9 (P/S/T-rich Dom.) antibody |
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| Rabbit Polyclonal Anti-NEK9 Antibody (Pro/Ser/Thr Rich Domain) | |||||||
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Rabbit | Aff - Purified | Hu | P |
50 µg /
€425.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341013 | NEK9 antibody |
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| Rabbit Polyclonal Anti-NEK9 Antibody (Pro/Ser/Thr Rich Domain) | |||||||
 |
Rabbit | Purified | Bov, Bt, Can, Hu, Ms, Rt |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA350922 | NEK9 antibody |
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| Rabbit Polyclonal Anti-NEK9 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA503326 | NEK9 antibody |
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| NEK9 mouse monoclonal antibody, clone OTI1H1 (formerly 1H1) | |||||||
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Mouse | IgG1 | OTI1H1 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA503367 | NEK9 antibody |
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| NEK9 mouse monoclonal antibody, clone OTI7G7 (formerly 7G7) | |||||||
 |
Mouse | IgG1 | OTI7G7 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP07466PU-N | NEK9 antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu | IP, P, WB |
50 µl /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| AP20364PU-N | NEK9 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP311326 | NEK9 |
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| Recombinant protein of human NIMA (never in mitosis gene a)- related kinase 9 (NEK9) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||