NET1 antibody
Principal name
NET1 antibody
Alternative names for NET1 antibody
ARHGEF8, Neuroepithelial cell-transforming gene 1 protein, p65 Net1 proto-oncogene, Rho guanine nucleotide exchange factor 8
Ncbi ID
9606, NP_001040625, NP_005854.2, NP_005854, NP_001040625.1, NP_001040624.1, NM_001047159, NM_019671, NM_005863, NM_001047160
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of NET1 antibody
- Kit Component:
- - KN310905G1, Net1 gRNA vector 1 in pCas-Guide vector
- KN310905G2, Net1 gRNA vector 2 in pCas-Guide vector
- KN310905D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Chan,A.M., (2007) Mol. Cell. Biol. 27 (24), 8683-8697
NET1 polyclonal antibody ( Cat # PAB6510 ) staining ( 2.5 µg/ml ) of paraffin embedded human tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
NET1 polyclonal antibody ( Cat # PAB7041 ) ( 2.5 µg/ml ) staining of paraffin embedded human breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot analysis of NET1 expression in transfected 293T cell line (H00010276-T02) by NET1 MaxPab polyclonal antibody.Lane 1: NET1 transfected lysate(61.90 KDa).Lane 2: Non-transfected lysate.
Human Jurkat; WB Suggested Anti-NET1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; NET1 antibody - middle region (ARP52123_P050) in Human Jurkat cells using Western Blot
Human HEK293T; WB Suggested Anti-NET1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate. NET1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.; NET1 antibody - N-terminal region (ARP52122_P050) in Human HEK293T cells using Western Blot
(2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Predicted band size: 68 kDa . Positive control: Jurkat and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Jurkat cell lysate Lane1: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
WB Suggested Anti-NET1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysateNET1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells
WB Suggested Anti-NET1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate
Anti-NET1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NET1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA311345 | NET1 antibody |
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| Goat Anti-NET1 / ARHGEF8 (Internal) Antibody | |||||||
 |
Goat | Purified | Can, Hu, Ms, Rt | P, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA323281 | NET1 antibody |
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| Anti-NET1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA341074 | NET1 antibody |
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| Rabbit Polyclonal Anti-NET1 Antibody (Internal) | |||||||
 |
Rabbit | Purified | Hst, Hu, Mky, Ms, Rb, Rt |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TA341075 | NET1 antibody |
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| Rabbit Polyclonal Anti-NET1 Antibody (C-Terminus) | |||||||
 |
Rabbit | Purified | Hu, Mky |
50 µg /
€425.00
|
|||
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760514 | NET1 (N-term HIS tag, transcript variant 2) |
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| Purified recombinant protein of Human neuroepithelial cell transforming 1 (NET1), transcript variant 2, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR218791L2V | Lenti ORF particles, Net1 (GFP-tagged) - Mouse neuroepithelial cell transforming gene 1 (Net1), transcript variant 1, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,330.00
|
|
| OriGene Technologies, Inc. | ||
| MR208638L2V | Lenti ORF particles, Net1 (GFP-tagged) - Mouse neuroepithelial cell transforming gene 1 (Net1), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR208638L1V | Lenti ORF particles, Net1 (Myc-DDK-tagged) - Mouse neuroepithelial cell transforming gene 1 (Net1), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||